Evidence suggests that fasting exerts extensive antitumor effects in various cancers, including colorectal cancer (CRC). However, the mechanism behind this response is unclear. We investigate effect of on glucose metabolism and malignancy CRC. find upregulates expression a cholesterogenic gene, Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1), during inhibition CRC cell aerobic glycolysis proliferation. In addition, downregulation FDFT1 correlated with malignant progression poor prognosis...

// Pengfei Sun 1, * , Huihui Wang Zhiyong He Xiangyuan Chen 1 Qichao Wu Wankun Zhirong Meilin Weng Minmin Zhu Duan Ma 2 and Changhong Miao Department of Anesthesiology, Fudan University Shanghai Cancer Center, Oncology, Medical College, University, Shanghai, China Key Laboratory Metabolism Molecular Medicine, Ministry Education, Biochemistry Biology, Collaborative Innovation Center Genetics Development, Institutes Biomedical Sciences, School Basic These authors have contributed equally to...

Abstract Hepatocellular carcinoma (HCC) is one of the most aggressive cancers worldwide. Despite such a public health importance, efficient therapeutic agents are still lacking for this malignancy. Most tumor cells use aerobic glycolysis to sustain anabolic growth, including HCC, and preference often leads close association with poorer clinical outcomes. The histone methyltransferase SET8 plays crucial roles in controlling cell-cycle progression, transcription regulation, tumorigenesis....

Abstract Background Dopamine and dopamine receptor D1 (DRD1), a member of the family, have been indicated to play important roles in cancer progression, but secretion hepatocellular carcinoma (HCC) effects DRD1 on HCC remain unclear. This study was designed explore contribution dopaminergic system determine relationship between prognosis patients. Methods The metabolic monitored using enzyme‐linked immunosorbent assays (ELISAs). expression detected by microarray analysis,...

Cyclooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS) overexpression results in endothelial apoptosis, thus mediating vascular injury hyperglycaemia. E26 transformation-specific sequence transcription factor-1 (ESE-1), which belongs to the family of factors, has been demonstrated be involved COX2 iNOS gene transcription. Our previous study indicated that SET domain-containing protein 8 (SETD8) downregulation is high glucose-mediated inflammation human umbilical vein cells...

Diabetic nephropathy (DN) has become the common and principal microvascular complication of diabetes that could lead to end-stage renal disease. It was reported endothelial-to-mesenchymal transition (EndMT) in glomeruli plays an important role DN. Enolase1 (ENO1) Lysine Methyltransferase 5A (KMT5A) were found modulate epithelial-to-mesenchymal some situations. In present study, we speculated KMT5A regulates ENO1 transcript, thus participating hyperglycemia-induced EndMT Our study represented...

Gamma-Aminobutyric Acid Type B Receptor (GABABR) plays essential roles in tumor progression.However, the function of GABABR colorectal cancer (CRC) needs further clarification.As main part GABABR, GABABR1 expression was identified significantly lower tissues than those non-tumor normal and that CRC patients with high lived longer.Further studies indicated knockdown elevated cell proliferation, migration, invasion.Furthermore, activated epithelial-mesenchymal transition (EMT)-related proteins...

Abstract Diabetic nephropathy (DN) is the primary microvascular complication of diabetes mellitus and may result in end-stage renal disease. The overproduction various inflammatory factors involved pathogenesis DN. Protein tyrosine phosphatase 1B (PTP1B) modulates expression a series cytokines nuclear factor kappa B (NF-κB) activity. cAMP response element-binding protein (CREB) lysine methyltransferase 5A (KMT5A) have been reported to participate maintenance healthy endothelium. In present...

Diabetic nephropathy (DN) is currently the leading cause of end-stage renal disease globally. The endothelial-to-mesenchymal transition (EndMT) glomerular endothelial cells has been reported to play a crucial role in DN. As specific form epithelial-to-mesenchymal transition, EndMT and may exhibit mutual modulators. Profilin 2 (PFN2) participate transition. Moreover, ETS proto-oncogene 1 (ets1) lysine methyltransferase 5A (KMT5A) have contribute high glucose-mediated injury In this study, we...

Recent studies have highlighted a connection between gut microbiota and hypertension, yet the precise nature of this relationship remains unclear. This research aims to analyze causal link along with associated complications, utilizing two-sample bidirectional Mendelian randomization (MR). Summary data from genome-wide association (GWAS) meta-analyses, including GWAS 24 cohorts, latest for hypertension-related conditions were acquired. Employing various MR methods, Inverse-variance weighted...

Abstract Diabetic kidney disease (DKD), a primary microvascular complication arising from diabetes, may result in end‐stage renal disease. Epigenetic regulation of endothelial mesenchymal transition (EndMT) has been recently reported to exert function metabolic memory and DKD. Here, we investigated the mechanism which Sirt7 modulated EndMT human glomerular cells (HGECs) occurrence Lower levels SDC1 were noted glomeruli both DKD patients diabetes‐induced injury rats, as well with high blood...

Hyperglycemia could induce oxidative stress, activate transcription factor nuclear kappa B (NF-κB), up-regulate expression of endothelial adhesion molecules, and lead to injury. Studies have indicated that propofol attenuate stress suppress NF-κB activation in some situations. In the present study, we examined whether how improved high glucose-induced up-regulation molecules human umbilical vein cells (HUVECs).Protein NF-κB, inhibitory subunit NF-κBα (IκBα), protein kinase Cβ2 (PKCβ2),...

Many studies have demonstrated the crucial roles of 5-methylcytosine (m5C) RNA methylation in cancer pathogenesis.Two datasets, including TCGA-KIRP and ICGC, related clinical information were downloaded, where expression 13 m5C regulators was examined. We applied LASSO regression to construct a multi-m5C-regulator-based signature TCGA cohort, which further validated using ICGC cohort. Univariate multivariate Cox regressions evaluate independent prognostic value our model. The differences...

Perioperative hyperglycemia is a common clinical metabolic disorder. Hyperglycemia could induce endothelial apoptosis and dysfunction. Propofol widely used IV anesthetic drug in settings. In the present study, we examined whether how propofol reduced high glucose-induced dysfunction human umbilical vein cells (HUVECs).HUVECs were cultured with different concentrations (5, 10, 15, 25 mM) of glucose for times (4, 8, 12, 24 hours). To study effect propofol, incubated (0.2, 1, 5, μM) 2 hours....

Abstract This study aimed to investigate the protective effects of oridonin (ORI) on cecal ligation and puncture (CLP)‐induced sepsis in mice. Male C57BL/6 mice weighing 22–30 g aged 8–10 weeks were randomly assigned three groups: Sham group, CLP or plus ORI group. In group was induced, intraperitoneal injection normal saline (100 μg/kg) conducted, respectively. The survival rate determined within following 7 days. blood, liver, lung collected at 24 hours after injury. Hematoxylin–eosin...

Diabetic nephropathy (DN) is regarded as the main vascular complication of diabetes mellitus, directly affecting outcome diabetic patients. Inflammatory factors were reported to participate in progress DN. Wingless-type family member 5 (WNT5A), myeloid zinc finger 1 (MZF1), and lysine methyltransferase 8 (SETD8) have also been elevate inflammatory factor levels activate nuclear kappa B (NF-κB) pathway induce endothelial dysfunction. In current study, it was assumed that MZF1 associates with...

Colorectal cancer (CRC) is a major disease that threatens human health. It has been reported the acyl-coenzyme A (CoA): cholesterol acyltransferase 2 (ACAT2) gene can promote progression of hepatocellular carcinoma, but its function in CRC still unclear. In this study, we aimed to elucidate ACAT2 CRC.Western blot and qPCR were used detect relative level tissue adjacent non-cancerous tissues, then association between expression clinicopathological features survival patients assessed. The CT26...

Background . Hepatocellular carcinoma (HCC) is a life-threatening cancer, and the Kelch-like ECH-associated protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2)/antioxidant response element (ARE) signalling pathway plays crucial role in apoptosis resistance cancer cells. Fasting reported to mediate tumour growth reduction apoptosis. SET8 involved proliferation, invasiveness, migration. However, whether participates fasting-mediated HCC remains unclear. Methods We used immunohistochemical staining...

Background . Colorectal cancer (CRC) is one of the leading causes death worldwide. Successful treatment CRC relies on accurate early diagnosis, which currently a challenge due to its complexity and personalized pathologies. Thus, novel molecular biomarkers are needed for detection. Methods Gene microRNA microarray profiling tissues miRNA-seq data were analyzed. Candidate predicted using both CRC-specific network miRNA-BD tool. Validation analyses carried out interrogate identified candidate...

Membranous nephropathy (MN) and IgA (IgAN) pose challenges in clinical treatment with existing therapies primarily focusing on symptom relief often yielding unsatisfactory outcomes. The search for novel drug targets remains crucial to address the shortcomings managing both kidney diseases.

Colorectal cancer (CRC) is one of the most fatal cancers digestive system. Although stem cells and metabolic reprogramming have an important effect on tumor progression drug resistance, their combined CRC prognosis remains unclear. Therefore, we generated a 21-gene mRNA stemness index-related risk score model, which was examined in The Cancer Genome Atlas Gene Expression Omnibus databases (1323 patients) validated using Zhongshan Hospital cohort (200 patients). high-risk group showed more...