A5101441882- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Metabolism and Genetic Disorders
- Breast Cancer Treatment Studies
- Breast Lesions and Carcinomas
- Autism Spectrum Disorder Research
- Cancer-related molecular mechanisms research
- Genomics and Rare Diseases
- Mitochondrial Function and Pathology
- Cancer-related gene regulation
- Peroxisome Proliferator-Activated Receptors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- Genetic Syndromes and Imprinting
- Congenital heart defects research
- Adipose Tissue and Metabolism
- Cancer Risks and Factors
- RNA Interference and Gene Delivery
- Child Nutrition and Water Access
- Ion channel regulation and function
- Nanoparticle-Based Drug Delivery
- Circular RNAs in diseases
Singapore Institute for Clinical Sciences
2012-2024
Agency for Science, Technology and Research
2012-2024
Peking University First Hospital
2013-2024
Peking University
2014-2024
Wuhan National Laboratory for Optoelectronics
2022-2024
Huazhong University of Science and Technology
2022-2024
Institute of Hematology & Blood Diseases Hospital
2020-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2024
Nanjing Medical University
2013-2024
First People's Hospital of Yuhang District
2024
Abstract Direct sequencing of exons 3 to 35 and the exon–intron boundaries CACNA1H gene was conducted in 118 childhood absence epilepsy patients Han ethnicity recruited from North China. Sixty‐eight variations have been detected gene, and, among identified, 12 were missense mutations only found 14 a heterozygous state, but not any 230 unrelated controls. The identified occurred highly conserved residues T‐type calcium channel gene. Our results suggest that might be an important...
Integrating the genotype with epigenetic marks holds promise of better understanding biology that underlies complex interactions inherited and environmental components define developmental origins a range disorders. The quality in utero environment significantly influences health over lifecourse. Epigenetics, particular DNA methylation marks, have been postulated as mechanism for enduring effects prenatal environment. Accordingly, neonate methylomes contain molecular memory individual...
Triple-negative breast cancer (TNBC) is highly invasive and aggressive lacks specific molecular targets to improve the prognosis. MiR-25-3p promotes proliferation of many tumors its role underlying mechanisms in TNBC remain be well elucidated.Differential expression miR-25-3p was measured with quantitative real-time PCR (qRT-PCR) both tissues cell lines validated Cancer Genome Atlas (TCGA) database. The effects on proliferation, apoptosis capacity were evaluated using Cell counting kit-8...
Childhood obesity is a major public health issue. Here we investigated whether differential DNA methylation was associated with childhood obesity. We studied profiles in whole blood from 78 obese children (mean BMI Z-score: 2.6) and 71 age- sex-matched controls 0.1). samples control groups were pooled analyzed using the Infinium HumanMethylation450 BeadChip array. Comparison of between subjects revealed 129 differentially methylated CpG (DMCpG) loci 80 unique genes that had greater than 10%...
Maternal obesity reduces adipogenic progenitor density in offspring adipose tissue. The ability of tissue expansion the obese mothers is limited and associated with metabolic dysfunction when challenged a high-fat diet. induces DNA demethylation promoter zinc finger protein 423, which renders cells high capacity. demonstrates long-term effects on capacity tissue, demonstrating developmental programming effect.Maternal (MO) programs disorders, although underlying mechanisms remain poorly...
Early life environments interact with genotype to determine stable phenotypic outcomes. Here we examined the influence of a variant in brain-derived neurotropic factor (BDNF) gene (Val66Met), which underlies synaptic plasticity throughout central nervous system, on degree antenatal maternal anxiety associated neonatal DNA methylation. We also association between methylation and brain substructure volume, as function BDNF genotype. Infant, but not maternal, dramatically influences epigenome...
Aim: Determine if the association of HIF3A DNA methylation with weight and adiposity is detectable early in life. Material & methods: We determined genotype patterns (on hybridization arrays) extracted from umbilical cords 991 infants. Methylation levels at three CpGs first intron were related to neonatal infant anthropometry nearby polymorphic sites. Results conclusion: Higher previously described associated greater adiposity. The effect sizes slightly smaller than those reported for adult...
Previous studies have demonstrated that long non‑coding RNA (lncRNA) is involved in vascular remodeling. The metastasis‑associated lung adenocarcinoma transcript 1 (MALAT1) lncRNA associated with the proliferation and migration of smooth muscle endothelial cells; however, its biological role pulmonary artery hypertension (PAH) currently unclear. aim present study was to investigate post‑transcriptional regulation MALAT1 human cells (HPASMCs). results revealed expression levels were...
The Infinium Human Methylation450 BeadChip Array (TM) (Infinium 450K) is an important tool for studying epigenetic patterns associated with disease. This array offers a high-throughput, low cost alternative to more comprehensive sequencing-based methodologies. Here we compare data generated by interrogation of the same seven clinical samples 450K and reduced representation bisulfite sequencing (RRBS). largest set comparing RRBS methodology reported so far. We show good agreement between two...
Interindividual variability in the epigenome has gained tremendous attention for its potential pathophysiological investigation, disease diagnosis, and evaluation of clinical intervention. DNA methylation is most studied epigenetic mark epigenome-wide association studies (EWAS) as it can be detected from limited starting material. Infinium 450K array popular platform high-throughput profiling this samples, cost-effective requires small amounts DNA. However, method suffers low genome coverage...
Triple-negative breast cancer (TNBC) has a poorer outcome than other subtypes of cancer, and the discovery dysregulated microRNA (miRNA) their role in tumor progression provided new avenue for elucidating mechanism involved TNBC. In this study, we identified that miR-3178 was significantly reduced TNBC, low expression correlated with poor overall survival TNBC but not non-TNBC. The ectopic overexpression suppressed cell proliferation, invasion, migration by inhibiting...
As the key material for semiconductor manufacturing, production technology of a photoresist (PR) is complex, with many varieties and specifications. Therein, synthesis current resins photosensitizers used preparation i-line complicated, reaction products may even cause lens pollution. Herein, kind photodegradable polyurethane (PU) positive simple system was designed prepared, in which photocleavable hexaarylbiimidazole (HABI) tert-butylhydroquinone (TBHQ) were as cross-linker radical...
Our previous studies demonstrated that S100A16 promotes adipogenesis and is involved in weight gain attenuation induced by dietary calcium. Till now, the function of breast cancer remains to be elucidated. In this study, we observed was expressed higher levels human tissues compared with paired adjacent non-cancerous tissues. Further examination showed overexpression MCF-7 cells could increase cell proliferation colony formation. One major mechanistic change able up-regulate transcription...
Aim: To investigate the effect of B12 and/or folic acid supplementation on genome-wide DNA methylation. Methods: We performed Infinium HumanMethylation450 BeadChip (Zymo Research, CA, USA) assay in children supplemented with (n = 12 each group) and investigated functional mechanism selected differentially methylated loci. Results: noted significant methylation changes postsupplementation (589 CpGs 2892 regions) + (169 3241 groups. Type 2 diabetes-associated genes TCF7L2 FTO; a miRNA, miR21...
Abstract Background The interplay among genetic, environment and epigenetic variation is not fully understood. Advances in high-throughput genotyping methods, high-density DNA methylation detection well-characterized sample collections, enable association studies at the genomic population levels (EWAS). field has extended to interrogate interaction of environmental genetic (GxE) influences on variation. Also, quantitative trait loci (methQTLs) their with health status enhanced our knowledge...
Mutations in the cyclin-dependent kinase-like 5 (CDKL5) (NM_003159.2) gene have been associated with early-onset epileptic encephalopathies or Hanefeld variants of RTT(Rett syndrome). In order to clarify CDKL5 genotype-phenotype correlations Chinese patients, mutational screening cases and RTT without MECP2 mutation were performed. The detailed clinical information including manifestation, electroencephalogram (EEG), magnetic resonance imaging (MRI), blood, urine amino acid organic 102...
Light quality is one of the key elicitors that directly affect plant cell growth and biosynthesis secondary metabolites. In this study, red callus spine grape was cultured under nine light qualities (namely, dark, white, red, yellow, blue, green, purple, warm-yellow, warm-white light). The effects different were studied on growth, accumulation phenolic compounds, total antioxidant capacity grape. results showed blue purple induced increased coloration in callus, whereas yellow greatest...
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is abnormally upregulated in various human cancers. However, the role of MALAT-1 acute myeloid leukemia (AML) remains unclear. This study investigated expression and function AML. MTT assay was used to determine cell viability, qRT-PCR applied RNA levels. Western blot performed detect protein expression. Flow cytometry conducted measure apoptosis. pull-down carried out interaction between METTL14. FISH localization METTL14 AML...