A5101871889- Influenza Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- interferon and immune responses
- DNA Repair Mechanisms
- Respiratory viral infections research
- Biochemical and Molecular Research
- Chemical Synthesis and Analysis
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Computational Drug Discovery Methods
- Melanoma and MAPK Pathways
- Carbohydrate Chemistry and Synthesis
- Immune Response and Inflammation
- Cancer therapeutics and mechanisms
- Natural product bioactivities and synthesis
- CRISPR and Genetic Engineering
- Click Chemistry and Applications
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Antimicrobial Resistance in Staphylococcus
- Microbial Natural Products and Biosynthesis
- Genetics and Neurodevelopmental Disorders
Oakwood Laboratories (United States)
2019-2021
Hospital for Sick Children
2011
Genesys (United States)
2003
California Institute of Technology
1996-2000
Howard Hughes Medical Institute
1998
Polyamide dimers containing three types of aromatic rings—pyrrole, imidazole, and hydroxypyrrole—afford a small-molecule recognition code that discriminates among all four Watson-Crick base pairs in the minor groove. The crystal structure specific polyamide dimer-DNA complex establishes structural basis for distinguishing T·A from A·T pairs. Specificity pair is achieved by means distinct hydrogen bonds between substituted pyrroles on ligand O2 thymine N3 adenine. In addition, shape-selective...
Pairing rules have been developed to predict the sequence specificity of minor groove binding polyamides containing pyrrole (Py) and imidazole (Im) amino acids. An Im/Py pair distinguishes G·C from C·G both these A·T/ T·A base pairs. A Py/Py appears not distinguish A·T To test extent this degeneracy, affinity orientation hairpin polyamide ImPyPy-γ-PyPyPy-β-Dp were measured for eight possible five 5'-TG(A,T)3-3' match sites. Affinity cleavage experiments using a with an EDTA·Fe(II) moiety at...
We describe the lead optimization and structure−activity relationship of DNA minor-groove binding ligands, a novel class antibacterial molecules. These compounds have been shown to target A/T-rich sites within bacterial genome and, as result, inhibit replication RNA transcription. The was focused on N-terminal aromatic heterocycles C-terminal amines resulted in with improved vivo tolerability excellent vitro potency (MIC ≥ 0.031 μg/mL) against broad range Gram-positive pathogens, including...
In order to determine whether there is an orientation preference of pyrrole−imidazole (Py−Im) polyamide dimers with respect the 5'−3' direction backbone in DNA helix, equilibrium association constants (Ka) were determined for a series six-ring hairpin polyamides which differ substitution at N and C termini. Affinity cleaving experiments using core sequence composition ImPyPy-γ-PyPyPy EDTA·Fe(II) moiety C-terminus reveal single binding each formal match site, 5'-(A,T)G(A,T)3-3'...
Abstract The overwhelming majority of Rett syndrome cases are caused by mutations in the gene MECP2 . has two isoforms, termed MECP2_e1 and MECP2_e2, which differ their N‐terminal amino acid sequences. A growing body evidence indicated that may be etiologically relevant isoform Syndrome based on its expression profile brain because, strikingly, no have been discovered affect MECP2_e2 exclusively. In this study we sought to characterize four classical patients with putatively only isoform....
Hairpin polyamides containing the aromatic amino acids 3-hydroxypyrrole (Hp), pyrrole (Py), and imidazole (Im) are capable of discriminating all four Watson−Crick base pairs in DNA minor groove according to a set pairing rules. Equilibrium association constants for eight-ring hairpins pairings Hp Py at single common position (ImImXPy-γ-ImYPyPy-β-Dp, where X/Y is Py/Py, Py/Hp, Hp/Py, Hp/Hp) were determined sites, 5'-TGGTCA-3', 5'-TGGACA-3', 5'-TGGCCA-3', 5'-TGGGCA-3', study relative binding...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTAffinity and Specificity of Multiple Hydroxypyrrole/Pyrrole Ring Pairings for Coded Recognition DNASarah White, James M. Turner, Jason W. Szewczyk, Eldon E. Baird, Peter B. DervanView Author Information Division Chemistry Chemical Engineering California Institute Technology Pasadena, California, 91125 Cite this: J. Am. Chem. Soc. 1999, 121, 1, 260–261Publication Date (Web):December 17, 1998Publication History Received25 August 1998Published...