A5003632285- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Parasite Biology and Host Interactions
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Effects and risks of endocrine disrupting chemicals
- Reproductive Biology and Fertility
- RNA Research and Splicing
- MicroRNA in disease regulation
- Evolution and Genetic Dynamics
- Marine Biology and Ecology Research
- Birth, Development, and Health
- Sperm and Testicular Function
- Marine Bivalve and Aquaculture Studies
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- T-cell and B-cell Immunology
- Aquatic Invertebrate Ecology and Behavior
- Insect symbiosis and bacterial influences
- Hormonal and reproductive studies
- Insect Resistance and Genetics
- Molecular Biology Techniques and Applications
City University of Hong Kong
2020-2025
National Health and Family Planning Commission
2024
Fudan University
2017-2024
City University of Hong Kong, Shenzhen Research Institute
2024
Central South University
2024
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2022-2023
Jiangnan University
2019-2022
Hong Kong Baptist University
2015-2022
Wuxi Fourth People's Hospital
2008-2022
Shanghai Institute of Planned Parenthood Research
2008-2021
Hydrothermal vents and methane seeps are extreme deep-sea ecosystems that support dense populations of specialized macro-benthos such as mussels. But the lack genome information hinders understanding adaptation these animals to inhospitable environments. Here we report genomes a vent/seep mussel (Bathymodiolus platifrons) shallow-water (Modiolus philippinarum). Phylogenetic analysis shows species diverged approximately 110.4 million years ago. Many gene families, especially those for...
In plants, transcripts move to distant body parts potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem distinct plant parts. Little is known regarding features, structural motifs, potential base modifications how these may affect their mobility. We identified Arabidopsis thaliana mRNAs harboring modified 5-methylcytosine (m5C) found that significantly enriched in previously described...
Third-generation sequencing can be used in human cancer genomics and epigenomic research. Oxford Nanopore Technologies (ONT) recently released R10.4 flow cell, which claimed an improved read accuracy compared to R9.4.1 cell. To evaluate the benefits defects of cell for profiling on MinION devices, we non-small-cell lung-carcinoma line HCC78 construct libraries both single-cell whole-genome amplification (scWGA) shotgun sequencing. The reads were benchmarked terms accuracy, variant detection,...
Obesity is a worldwide epidemic, and risk factor for cardiovascular disease type 2 diabetes. Consequently, the development of safe effective anti-obesity drugs an area ongoing clinical interest. MicroRNAs play vital role in by inhibiting expression genes involved adipogenesis lipogenesis. However, application miRNAs has been limited lack appropriate delivery systems. The discovery microvesicles (MVs) shed new light on search more efficient drug transport tools. In previous study, we...
Abstract The Scaly-foot Snail, Chrysomallon squamiferum , presents a combination of biomineralised features, reminiscent enigmatic early fossil taxa with complex shells and sclerites such as sachtids, but in recently-diverged living species which even has iron-infused hard parts. Thus the Snail is an ideal model to study genomic mechanisms underlying evolutionary diversification armour. Here, we present high-quality whole-genome assembly tissue-specific transcriptomic data, show that scale...
Endosymbiosis with chemosynthetic bacteria has enabled many deep-sea invertebrates to thrive at hydrothermal vents and cold seeps, but most previous studies on this mutualism have focused the only. Vesicomyid clams dominate global chemosynthesis-based ecosystems. They differ from symbiotic animals in passing their symbionts parent offspring, enabling intricate coevolution between host symbiont. Here, we sequenced genomes of clam Archivesica marissinica (Bivalvia: Vesicomyidae) its bacterial...
The family Ampullariidae includes both aquatic and amphibious apple snails. They are an emerging model for evolutionary studies due to the high diversity, ancient history, wide geographical distribution. Insight into drivers of ampullariid evolution is hampered, however, by lack genomic resources. Here, we report genomes four ampullariids spanning Old World (Lanistes nyassanus) New (Pomacea canaliculata, P. maculata, Marisa cornuarietis) clades. have conserved bilaterial karyotype features a...
Abstract Arabidopsis thaliana transcriptomes have been extensively studied and characterized under different conditions. However, most of the current ‘RNA-sequencing’ technologies produce a relatively short read length demand reverse-transcription step, preventing effective characterization transcriptome complexity. Here, we performed Direct RNA Sequencing (DRS) using latest Oxford Nanopore Technology (ONT) with exceptional length. We demonstrate that complexity A. has substantially...
Abstract Animals endemic to deep-sea hydrothermal vents often form obligatory symbioses with bacteria, maintained by intricate host–symbiont interactions. Most genomic studies on holobionts have not investigated both sides similar depths. Here, we report dual symbiosis in the peltospirid snail Gigantopelta aegis two gammaproteobacterial endosymbionts: a sulfur oxidiser and methane oxidiser. We assemble high-quality genomes for all three parties, including chromosome-level host genome....
Chemokine receptor-initiated signaling plays critical roles in cell differentiation, proliferation, and migration. However, the regulation of chemokine receptor under physiological pathological conditions is not fully understood. In present study, we demonstrate that CXC 4 (CXCR4) formed a complex with ferritin heavy chain (FHC) ligand-dependent manner. Our vitro binding assays revealed purified FHC associated both glutathione S-transferase-conjugated N-terminal C-terminal domains CXCR4,...
Asthenozoospermia (AS) is a common cause of human male infertility.In one study, more than 80% the samples from infertile men had reduced sperm motility.Seminal plasma mixture secretions testis, epididymis and several accessory glands, including prostate, seminal vesicles Cowper's gland.Studies have shown that contains proteins are important for motility.To further explore pathophysiological character AS, we separated AS patients healthy donors using sodium dodecyl sulfate polyacrylamide gel...
Environmental BPA exposure has been shown to impact human sperm concentration and motility, as well rodent spermatogenesis. However, it is unclear whether associated with alteration in DNA hydroxymethylation, a marker for epigenetic modification, sperm. A genome-wide hydroxymethylation study was performed using samples of men who were occupationally exposed BPA. Compared controls had no occupational exposure, the total levels 5-hydroxymethylcytosine (5hmc) increased significantly (19.37%...
Abstract Most next-generation sequencing platforms permit acquisition of high-throughput DNA sequences, but the relatively short read length limits their use in genome assembly or finishing. Illumina has recently released a technology called Synthetic Long-Read Sequencing that can produce reads unusual length, i.e., predominately around 10 Kb. However, systematic assessment finishing and is still lacking. We evaluate promise deficiency long these aspects using isogenic C. elegans with no...
The associations between Bisphenol-A (BPA) exposure and reproductive hormone levels among women are unclear. A cross-sectional study was conducted female workers from BPA-exposed unexposed factories in China. Women's blood samples were collected for assay of follicle-stimulating (FSH), luteinizing (LH), 17β-Estradiol (E2), prolactin (PRL), progesterone (PROG). Their urine BPA measurement. In the exposed group, time weighted average to an 8-h shift (TWA8), a measure incorporating historic...
Abstract The Challenger Deep in the Mariana Trench is deepest point oceans of our planet. Understanding how animals adapt to this harsh environment characterized by high hydrostatic pressure, food limitation, dark and cold great scientific interest. Of dwelling Deep, amphipods have been captured using baited traps. In study, we sequenced transcriptome amphipod Hirondellea gigas collected at a depth 10,929 m from East Pond Deep. Assembly these sequences resulted 133,041 contigs 22,046...
Massively parallel sequencing of the polyadenylated RNAs has played a key role in delineating transcriptome complexity, including alternative use an exon, promoter, 5' or 3' splice site polyadenylation site, and RNA modification. However, reads derived from current RNA-seq technologies are usually short deprived information on modification, compromising their potential defining complexity. Here, we applied direct method with ultralong using Oxford Nanopore Technologies to study complexity...
The chemokine receptor CXCR4-mediated signaling cascades play an important role in cell proliferation and migration, but the underlying mechanisms by which is regulated remain incompletely understood. Here, we demonstrate that CXCR4 was co-immunoprecipitated with cyclophilin A (CyPA) from lysate of HEK293 cells stably expressing CXCR4. Although both glutathione S-transferase-CXCR4 N- C-terminal fusion proteins were associated purified CyPA, truncation domain robustly inhibited...
Abstract Objective Osteopontin (OPN) is a proinflammatory protein with critical role in leukocyte migration. Although OPN has been implicated rheumatoid arthritis (RA), its underlying mechanism remains unknown. In this study, we investigated the and molecular of induction 2 key chemokines, monocyte chemoattractant 1 (MCP‐1) macrophage inflammatory 1β (MIP‐1β), RA. Methods Enzyme‐linked immunosorbent assay quantitative polymerase chain reaction were used to determine chemokine expression....
Abstract Objective Osteopontin (OPN) that is aberrantly produced in rheumatoid synovium thought to play an important role arthritis (RA). This study was undertaken investigate the of OPN differentiation and accumulation Th17 cells synovium. Methods Peripheral blood mononuclear purified CD4+ T derived from patients with RA or healthy controls were used test effect vitro. Cytokine expression determined by enzyme‐linked immunosorbent assay quantitative polymerase chain reaction. Intracellular...
Background Prostate cancer (PCa) is the second most common solid among men worldwide and fifth leading cause of cancer-related deaths in men. Sulforaphane (SFN), an isothiocyanate compound, has been shown to exert inhibitory effects on a variety cancers. However, biological function SFN PCa not fully elucidated. The objective this study was conducted further investigate possible underlying mechanism using vitro cell culture vivo tumor model experiments. Methods Cell viability, migration,...
Abstract Bacterial gene expression is a complex process involving extensive regulatory mechanisms. Along with growing interests in this field, Nanopore Direct RNA Sequencing (DRS) provides promising platform for rapid and comprehensive characterization of bacterial biology. However, the DRS currently deficient yield mRNA-mapping reads has yet to be exploited transcriptome-wide modification mapping. Here, we showed that pre-processing total (size selection followed by ribosomal depletion...