Dietary supplementation of butyrate can prevent diet-induced obesity through increasing mitochondrial function in mice, yet the up-stream signaling pathway remains elusive. In this study, weaned mice were divided into two groups, fed control (CON) and high-fat diet (HF, 45% energy from fat), respectively, for 8 weeks. HF-induced obese maintained on HF diet, then groups; HFB group was gavaged with 80 mg sodium (SB) per every other day 10 days, while received vehicle. It shown that five gavage...

Obesity is a worldwide epidemic, and risk factor for cardiovascular disease type 2 diabetes. Consequently, the development of safe effective anti-obesity drugs an area ongoing clinical interest. MicroRNAs play vital role in by inhibiting expression genes involved adipogenesis lipogenesis. However, application miRNAs has been limited lack appropriate delivery systems. The discovery microvesicles (MVs) shed new light on search more efficient drug transport tools. In previous study, we...

The deposition of fat in muscle, recognized by the consumer as marbling, is an important meat quality trait. objective study was to provide additional insights into quantitative extent marbling means computer image analysis. Fifty-one F(2) generation German Holstein and Charolais crossbreed cattle, 18 mo age, were used determine relationships among traits, adipocyte size, amount adipose tissue different depots. Differences recorded size i.m. adipocytes groups flecks, divided according...

Myostatin (MSTN) is suggested to mediate the effect of maternal nutrition on offspring phenotype, yet mechanisms underlying such adaptive gene regulation elusive. In this study, we determined effects dietary protein transcriptional MSTN in skeletal muscle pig offspring. Fourteen Meishan sows were fed either low-protein (LP) or standard-protein (SP) diets throughout gestation and lactation. expression longissimus dorsi was both at weaning finishing stages. mRNA abundance downregulated...

Our previous studies demonstrated that dietary daidzein improves egg production in ducks during the late period of laying cycle. The present study was aimed to investigate effect hens, with more focus on eggshell quality. expression ER-α, GH-R, and IGF-IR mRNA shell glands determined identify target genes action reveal relationship between quality profiles gene hens. 1000 ISA at 445 days age, were allotted random two groups given basal diet or without 10 mg per kg for 9 weeks. Daidzein...

Four hundred fifty 52-wk-old Langshan layer hens (dual-purpose type, an indigenous poultry breed of China) were randomly divided into 9 treatments with 5 replicates in each treatment. Birds fed corn-soybean diets (0.13 mg Se/kg) supplemented 0, 0.30, and 0.60 mg/kg Se from yeast 3.2, 4.0, 5.4 g dl-Met/kg, respectively. Increasing supplementation significantly increased concentration the egg yolk (P < 0.01) 3.2 Met/kg treatment was higher than those 4.0 treatments. Adding 0.3 Se/kg to diet...

We have shown previously that microvesicle (MV)-delivered miR-130b (miR-130b-MV) is able to target PPAR-γ and subsequently reduce the lipid accumulation in vitro. However, vivo effect of on fat deposition glucose homeostasis remains unknown. Three-week-old C57BL/6 mice were fed a high-fat diet for 8 weeks then intravenously injected with MV-packaged scrambled control microRNA (miRNA) or every other day 10 days. Glucose tolerance test was performed body weight, epididymal as well expression...

White adipose tissue is recognized as a highly active organ, which closely related to large number of physiological and metabolic processes besides storing triglycerides. However, little known regarding the response acute inflammation. Therefore, in this study we employed growing pigs investigate changes lipid metabolism proteome white after lipopolysaccharide (LPS) stimulation model for bacterial infection.The expression inflammation genes was determined by quantitative real-time polymerase...

Our previous study had shown that chronic corticosterone (CORT) exposure causes excessive fat deposition in chicken liver, yet it remains unknown whether is associated with inflammation and fibrosis. In general, heat shock proteins (HSPs) are activated response to acute stress play a cytoprotective role, this activation m6A-mediated post-transcriptional regulation. However, changes of HSPs the m6A methylation on their mRNAs CORT treatment liver have not been reported. study, induced fibrosis...

Light management plays an important role in the growth and behavior of broiler chickens. Constant light early post hatch stage has been a common practice industry for improving performance, while whether how constant life affects chickens is rarely reported. In this study, newly hatched chicks were kept either (24 L:0 D, LL) or (12 L:12 LD) photoperiod 7 d then maintained 12 D thereafter until 21 days age. increased average daily feed intake but not body weight, which led to higher...

Summary In order to identify the genes involved in fatness variability, we studied expression of several implicated hepatic lipid metabolism broiler chickens with different fat deposition patterns during embryonic development. The mRNA acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), malic enzyme (ME) and apolipoprotein B100 (apoB100) were determined using reverse transcriptase‐polymerase chain reaction (RT‐PCR). Samples livers collected from Arbor Acres (AA) Sanhuang (SH) on day 9,...

Corticotropin-releasing hormone (CRH), the major secretagogue of hypothalamic-pituitary-adrenal (HPA) axis, is intricately intertwined with clock genes to regulate circadian rhythm various body functions. N6-methyladenosine (m6A) RNA methylation involved in regulation rhythm, yet it remains unknown whether CRH expression and m6A modification oscillate chicken hypothalamus how rhythms change under chronic stress.Chronic exposure corticosterone (CORT) eliminated diurnal patterns plasma CORT...

Abstract The objective of present study was the comparison trans ‐11 18:1 (VA) and cis ‐9, CLA concentrations in rumen different tissues beef cattle, to examine diet breed effects on compound concentration deposition. Sixty‐four German Holstein Simmental bulls were randomly assigned two dietary treatments, based concentrate or pasture. VA rumen, duodenal digesta determined by gas chromatography. results showed that pasture relative feeding significantly increased digesta, plasma erythrocyte...

Cysteamine has been reported to modulate energy homeostasis and exert significant growth-promoting effects in broiler chickens. However, little is known concerning its on egg production of hens the growth rate their offspring. In present study, 67-wk-old breeders were allotted at random control cysteamine-supplemented (400 mg/kg) groups for 8 wk. The hatchlings fed under same condition until 6 wk age. significantly increased average laying by 2.24% (P < 0.01), decreased dramatically...

Breed differences in adult animals are determined during fetal development.If interventions to be developed that influence growth of muscle and fat, it is important know at which time gestation breed appear fixed.The objective this study was characterize development cattle different breeds.Pregnant cows 4 breeds with impetus muscularity were slaughtered under normal processing conditions the fetuses removed.German Angus, a typical beef cattle; Galloway, smaller, environmentally resistant...