A5020140861- Cancer Mechanisms and Therapy
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Plant Virus Research Studies
- PI3K/AKT/mTOR signaling in cancer
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Multiple Sclerosis Research Studies
- Liver physiology and pathology
- MicroRNA in disease regulation
- Protein Tyrosine Phosphatases
- Hepatocellular Carcinoma Treatment and Prognosis
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Cytomegalovirus and herpesvirus research
- Lung Cancer Treatments and Mutations
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Peptidase Inhibition and Analysis
- Plant Disease Resistance and Genetics
- Ubiquitin and proteasome pathways
First Affiliated Hospital of Jinan University
2021-2024
Jinan University
2020-2024
Institute of Hydrobiology
2024
Xuzhou Medical College
2024
First Affiliated Hospital of Anhui Medical University
2012-2023
Anhui Medical University
2012-2023
Army Medical University
2022
Southwest Hospital
2022
Shanghai Jiao Tong University
2006-2022
Shanghai Chest Hospital
2021-2022
In 2017, surveillance for tickborne diseases in China led to the identification of a patient who presented hospital Inner Mongolia with febrile illness that had an unknown cause. The clinical manifestation was similar encephalitis virus (TBEV) infection, but neither TBEV RNA nor antibodies against were detected.We obtained blood specimen from index and attempted isolate identify causative pathogen, using genome sequence analysis electron microscopy. We also initiated heightened program same...
Copolymer-I (COP-I) has unique immune regulatory properties and is a treatment option for multiple sclerosis (MS). This study revealed that COP-I induced the conversion of peripheral CD4 + CD25 - to T cells through activation transcription factor Foxp3. led significant increase in Foxp3 expression MS patients whose was reduced at baseline. cell lines generated by expressed high levels correlated with an increased potential. Furthermore, we demonstrated induction mediated its ability produce...
Abstract Viral infections are though to play an important role in the pathogenesis of multiple sclerosis (MS) potentially through molecular mimicry. An identical sequence was found both myelin basic protein (MBP, residues 96–102), a candidate autoantigen for MS, and human herpesvirus‐6 (HHV‐6 U24, 4–10) that is suspected viral agent associated with MS. In this study, we showed greater than 50% T cells recognizing MBP 93‐105 cross‐reacted could be activated by synthetic peptide corresponding...
The precise role of B cell surface immunoglobulin (slg) in the activation cells is unclear at present. In particular, it uncertain whether ligands interacting with slg suffice to induce proliferation, or simply a state which becomes responsive growth factors made by accessory cells. We have examined effects two ligands, Staphylococcus aureus Cowan strain I (SAC) and antihuman mu chain (anti-mu), interact on highly purified human peripheral blood tonsillar cultured low concentrations....
Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies resistant to current chemotherapies or radiotherapies, which makes it urgent identify new therapeutic targets for HCC. In this study, we found that checkpoint kinase 1 (CHK1) was frequently overexpressed and correlated with poor clinical outcome in patients We further showed CHK1 inhibitor GÖ6976 capable sensitizing HCC cells cisplatin, indicating may have oncogenic function phosphorylated tumor suppressor spleen...
Several genome-wide association studies (GWAS) have identified a genetic polymorphism associated with the gene locus for interleukin 28B (IL28B), type III interferon (IFN), as major predictor of clinical outcome in hepatitis C. Antiviral effects IFN family previously been shown against several viruses, including C virus (HCV), and resemble function I utilization intracellular Janus kinase signal transducer activator transcription (JAK-STAT) pathway. Effects unique to IL28B that would...
Spleen tyrosine kinase (SYK) plays a critical role in immune cell signaling pathways and has been reported as biomarker for human hepatocellular carcinoma (HCC). We sought to investigate the mechanism by which SYK promotes liver fibrosis evaluate therapeutic target fibrosis. evaluated cellular localization of association between expression fibrogenesis normal, hepatitis B virus (HBV)‐infected, C (HCV)‐infected non‐alcoholic steatohepatitis (NASH) tissue (n=36, 127, 22 30, respectively). A...
The current pandemic of coronavirus disease 2019 (COVID-19) has affected >160 million individuals to date, and caused millions deaths worldwide, at least in part due the unclarified pathophysiology this disease. Identifying underlying molecular mechanisms COVID-19 is critical overcome pandemic. Metabolites mirror progression an individual can provide extensive insights into their pathophysiological significance each stage We a comprehensive view metabolic characterisation sera from...
Vessel co-option has been demonstrated to mediate colorectal cancer liver metastasis (CRCLM) resistance antiangiogenic therapy. The current mechanisms underlying vessel have mainly focused on "hijacker" tumor cells, whereas the function of "hijackee" sinusoidal blood vessels not explored. Here, we found that occurrence in bevacizumab-resistant CRCLM xenografts was associated with increased expression fibroblast activation protein α (FAPα) co-opted hepatic stellate cells (HSCs), which...
Microalgae are promising feedstocks for production of renewable biofuels and value-added bioproducts. Temperature nitrogen supply important environmental nutritional factors affecting the growth metabolism microalgae, respectively. In this study, lipid accumulation filamentous microalgae Xanthonema hormidioides under different temperatures (5, 7, 10, 15, 20, 25, 27 30 °C) initial concentrations (3, 9, 18 mM) were investigated, its adaptive mechanisms tolerance to low temperature stress...
Abstract Autoreactive T cells of CD4 and CD8 subsets recognizing myelin basic protein (MBP), a candidate autoantigen, are thought to contribute play distinct roles in the pathogenesis multiple sclerosis (MS). In this study we identified four MBP-derived peptides that had high binding affinity HLA-A2 HLA-A24 characterized CD8+ cell responses their functional properties patients with MS. There were significantly increased 9-mer MBP peptides, particular MBP111–119 MBP87–95 HLA-A2, MS compared...
Osteopontin (OPN) is an extracellular matrix protein of pleiotropic properties and has been recently recognized as a potential inflammatory cytokine. In this study, we demonstrate, for the first time to our knowledge, that overexpression OPN in synovial T cells associated with local milieu acts important mediator amplification perpetuation rheumatoid synovitis. The study revealed mRNA expression was highly elevated CD4+ derived from patients RA, which correlated increased concentrations...
Osteopontin (OPN) is an extracellular matrix protein of pleiotropic properties and has been recently recognized as a potential inflammatory cytokine. In this study, we demonstrate, for the first time to our knowledge, that overexpression OPN in synovial T cells associated with local milieu acts important mediator amplification perpetuation rheumatoid synovitis. The study revealed mRNA expression was highly elevated CD4+ derived from patients RA, which correlated increased concentrations...
Abstract The high incidence of recurrence and the poor prognosis hepatocellular carcinoma ( HCC ) necessitate discovery new predictive markers invasion prognosis. In this study, we evaluated expression pattern two members a novel oncogene family, Musashi1 MSI 1) Musashi2 2) in 40 normal hepatic tissue specimens, 149 specimens their adjacent non‐tumourous tissues. We observed that 1 2 were significantly up‐regulated High levels detectable 37.6% (56/149) 49.0% (73/149) respectively, but rarely...
Deficiency in the TGF-β-induced regulatory T (iTreg) cell differentiation is associated with compromised immune homeostasis and plays a key role many autoimmune diseases. Therapeutic intervention to enhance situ iTreg has become promising treatment modality for Here we describe that development of inflammation experimental encephalomyelitis (EAE) selective impairment largely due increased production TNF-α. The neutralization TNF-α markedly increases differentiation, leading amelioration EAE,...
The induction of senescence for cancer treatment has provoked considerable interest recently. Metformin, a first-line drug diabetes mellitus type 2, appears to be associated with lower risk and improved outcomes in hepatocellular carcinoma (HCC). mechanism involved function metformin HCC is poorly understood. We show that low doses induced hepatoma cell characterized by accumulation senescence-associated β-galactosidase activity (SA-β-gal) the marker Dec1, whereas higher initiated apoptotic...
Abstract HCV replication disrupts normal endoplasmic reticulum (ER) function and activates a signaling network called the unfolded protein response (UPR). UPR is directed by three ER transmembrane proteins including ATF6, IRE1 PERK. increases TGF-β1 oxidative stress, which play important roles in liver fibrogenesis. has been shown to induce through generation of reactive oxygen species (ROS) p38 MAPK, JNK, ERK1/2 NFκB-dependent pathways. However, relationship between HCV-induced stress...
Long noncoding RNAs (lncRNAs) play a critical role in the regulation of many important cellular processes. However, mechanisms by which lncRNAs regulate viral infection and host immune responses are not well understood. We sought to explore lncRNA hepatitis C virus (HCV) interferon response. performed RNA sequencing (RNAseq) Huh7.5.1 cells with or without alpha (IFNα) treatment. Clustered regularly interspaced short palindromic repeats/Cas9 guide (gRNA) was used knock out selected genes. The...
Background Spinal cord injury (SCI) is a devastating disease that lacks effective treatment. Interestingly, recent studies indicated vagus nerve stimulation (VNS), neuromodulation widely used in variety of central nervous system (CNS) diseases, improved motor function recovery after SCI. But the exact underlying mechanism how VNS ameliorates SCI unclear. This study aimed to confirm efficacy and further explore potential therapeutic Method A T10 spinal compression model was established adult...
<b><i>Objective:</i></b> To define the in vitro effects of interferon beta 1a (IFN-β1a) on myelin basic protein (MBP)-reactive T cells and to determine its regulatory mechanism cytokine networks patients with MS. <b><i>Methods:</i></b> The proliferation production MBP-reactive T-cell clones were measured thymidine uptake assays ELISA respectively. precursor frequency was estimated a microwell culture system. <b><i>Results:</i></b> IFN-β inhibited established clones, which correlated enhanced...
Abstract Using subtractive cDNA cloning, we have isolated a series of clones that are differentially expressed between B and T lymphocytes. Whereas some the from known cell-specific genes, many them represent previously uncharacterized genes. One these unknown genes was denoted as BL34. Northern blot analysis performed with BL34 revealed 1.6-kb mRNA transcript present at low levels in RNA extracted resting lymphocytes, but whose expression markedly increased prepared mitogen-activated cells....
IFN-beta currently serves as one of the major treatments for MS. Its anti-inflammatory mechanism has been reported involving a shift in cytokine balance from Th1 to Th2 T-cell response against elements myelin sheath. In addition and groups, two other important pro-inflammatory cytokines, IL-17 osteopontin (OPN), are believed play roles CNS inflammation pathogenesis this study, we examined potential effects on regulation OPN MS patients. We found that used vitro at 0.5-3 ng/mL significantly...