Dahlene N. Fusco
A5053149768- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Animal Virus Infections Studies
- Vaccine Coverage and Hesitancy
- COVID-19 Impact on Reproduction
- Viral Infections and Vectors
- Liver Disease and Transplantation
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Systemic Lupus Erythematosus Research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Parasites and Host Interactions
- Climate Change and Health Impacts
- Parasite Biology and Host Interactions
- COVID-19 epidemiological studies
Tulane University
2020-2025
University Medical Center New Orleans
2024
Tulane Medical Center
2024
University Medical Center
2022
University Hospital and Clinics
2022
Massachusetts General Hospital
2010-2018
Harvard University
2010-2018
MGH Institute of Health Professions
2017
Center for Systems Biology
2017
Creative Research Enterprises (United States)
2016
Understanding gene expression requires the ability to follow fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression characterize movement in real time single mRNA-protein complexes (mRNPs) nucleus living mammalian cells. This mobility was not directed but governed by simple diffusion. Some mRNPs were partially corralled throughout nonhomogenous nuclear environment, no accumulation at subnuclear domains observed. Following energy deprivation,...
Several genome-wide association studies (GWAS) have identified a genetic polymorphism associated with the gene locus for interleukin 28B (IL28B), type III interferon (IFN), as major predictor of clinical outcome in hepatitis C. Antiviral effects IFN family previously been shown against several viruses, including C virus (HCV), and resemble function I utilization intracellular Janus kinase signal transducer activator transcription (JAK-STAT) pathway. Effects unique to IL28B that would...
Vaccine protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection wanes over time, requiring updated boosters. In a phase 2, open-label, randomized clinical trial with sequentially enrolled stages at 22 US sites, we assessed safety and immunogenicity of second boost monovalent or bivalent variant vaccines from mRNA protein-based platforms targeting wild-type, Beta, Delta Omicron BA.1 spike antigens. The primary outcome was pseudovirus neutralization titers 50%...
Responses to alpha interferon (IFN-α)-based treatment are dependent on both host and viral factors vary markedly among patients infected with different hepatitis C virus (HCV) genotypes (GTs). Patients GT3 viruses consistently respond better IFN than do GT1 viruses. The mechanisms underlying this difference not well understood. In study, we sought determine the effects of HCV NS5A proteins from signaling. We found that overexpression either or significantly inhibited IFN-induced...
Abstract HCV replication disrupts normal endoplasmic reticulum (ER) function and activates a signaling network called the unfolded protein response (UPR). UPR is directed by three ER transmembrane proteins including ATF6, IRE1 PERK. increases TGF-β1 oxidative stress, which play important roles in liver fibrogenesis. has been shown to induce through generation of reactive oxygen species (ROS) p38 MAPK, JNK, ERK1/2 NFκB-dependent pathways. However, relationship between HCV-induced stress...
Background. We and others have shown that primary hepatitis C (HCV) infection in men infected with human immunodeficiency virus (HIV) causes early-onset liver fibrosis; however, little is known about the long-term natural history of disease these HIV-infected men. Methods. followed a cohort HCV New York City. Results. Four who were not cured after their developed decompensated cirrhosis within 17 months to 6 years infection. Three died 8 infection, 1 survived transplant done 2 4 had AIDS at...
A 59-year-old male with follicular lymphoma treated by anti-CD20-mediated B-cell depletion and ablative chemotherapy was hospitalized a COVID-19 infection. Although the patient did not develop specific humoral immunity, he had mild clinical course overall. The failure of all therapeutic options allowed infection to persist nearly 300 days active accumulation SARS-CoV-2 virus mutations. As rescue therapy, an infusion REGEN-COV (10933 10987) anti-spike monoclonal antibodies performed 270 from...
ABSTRACT Background Protection from SARS-CoV-2 vaccines wanes over time and is compounded by emerging variants including Omicron subvariants. This study evaluated safety immunogenicity of variant vaccines. Methods phase 2 open-label, randomized trial enrolled healthy adults previously vaccinated with a primary series single boost. Eligible participants were to one six Moderna COVID19 mRNA vaccine arms (50µg dose): Prototype (mRNA-1273), BA.1+Beta (1 or doses), BA.1+Delta, BA.1 monovalent,...
Abstract In a randomized clinical trial, we compare early neutralizing antibody responses after boosting with bivalent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) messenger RNA (mRNA) vaccines based on either BA.1 or BA.4/BA.5 Omicron spike protein combined wild-type spike. Responses against SARS-CoV-2 variants exhibited the greatest reduction in titers currently circulating subvariants for both vaccines.
Few antiviral therapies have been studied in patients with coronavirus disease 2019 (COVID-19) and kidney impairment. Herein, the efficacy, safety, pharmacokinetics of remdesivir, its metabolites, sulfobutylether-β-cyclodextrin excipient were evaluated hospitalized COVID-19 severe In REDPINE, a phase 3, randomized, double-blind, placebo-controlled study, participants aged ≥12 years for pneumonia acute injury, chronic disease, or failure randomized 2:1 to receive intravenous remdesivir (200...
Abstract Background COVID-19 continues to cause morbidity. Obeldesivir (ODV) is an oral, broad spectrum, nucleoside analog prodrug inhibitor of SARS-CoV-2 RNA-dependent RNA polymerase. Methods OAKTREE was a Phase 3, randomized, double-blind, placebo (PBO)-controlled study in adults and adolescents without risk factors for progression severe COVID-19. Participants were enrolled within 3 days symptom onset randomized 1:1 receive 350 mg ODV or PBO twice day 5 days. Primary endpoints time...
Flaviviral infections including dengue virus are an increasing clinical problem worldwide. Dengue infection triggers host production of the type 1 IFN, IFN alpha, one strongest and broadest acting antivirals known. However subverts signaling at early steps signal transduction. This subversion allows unbridled viral replication which subsequently ongoing which, again, is subverted. Identification downstream antiviral effectors will provide targets could be activated to restore broad activity,...
Human immunodeficiency virus (HIV)/hepatitis C (HCV) coinfection accelerates progressive liver fibrosis; however, the mechanisms remain poorly understood. HCV and HIV independently induce profibrogenic markers transforming growth factor beta-1 (TGFβ1) (mediated by reactive oxygen species [ROS]) nuclear kappa-light-chain-enhancer of activated B cells (NFκB) in hepatocytes hepatic stellate monoculture; they do not account for cellular crosstalk that naturally occurs. We created an vitro...
ABSTRACT Hepatitis C virus (HCV) infection has been shown to regulate microRNA 130a (miR-130a) in patient biopsy specimens and cultured cells. We sought identify miR-130a target genes explore the mechanisms by which regulates HCV hepatitis B (HBV) replication. used bioinformatics software, including miRanda, TargetScan, PITA, RNAhybrid, predict potential genes. its were overexpressed or knocked down use of small interfering RNA (siRNA) clustered regularly interspaced short palindromic repeat...
Many countries in sub-Saharan Africa have experienced lower COVID-19 caseloads and fewer deaths than other regions worldwide. Under-reporting of cases a younger population could partly account for these differences, but pre-existing immunity to coronaviruses is another potential factor. Blood samples from Sierra Leonean Lassa fever Ebola survivors their contacts collected before the first reported were assessed using enzyme-linked immunosorbent assays presence antibodies binding proteins...
ABSTRACT The elongation factor Tu GTP binding domain-containing protein 2 (EFTUD2) was identified as an anti-hepatitis C virus (HCV) host in our recent genome-wide small interfering RNA (siRNA) screen. In this study, we sought to further determine EFTUD2's role HCV infection and investigate the interaction between EFTUD2 other regulators involved innate immune (RIG-I, MDA5, TBK1, IRF3) JAK-STAT1 pathways. We found that decreased expression of viral sensors RIG-I MDA5 HCV-infected Huh7...