A5005895051- Hepatitis C virus research
- Mosquito-borne diseases and control
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Liver Disease Diagnosis and Treatment
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- HIV Research and Treatment
- Inflammasome and immune disorders
- Cellular transport and secretion
- Plant Virus Research Studies
- Respiratory viral infections research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Virology and Viral Diseases
- Ubiquitin and proteasome pathways
- Viral Infections and Outbreaks Research
- Endoplasmic Reticulum Stress and Disease
Shandong First Medical University
2019-2025
Shandong Provincial QianFoShan Hospital
2021-2025
Shandong Provincial Hospital
2020-2024
Xinjiang New Energy Research Institute (China)
2024
National Health and Family Planning Commission
2020
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2012-2019
Qingdao University
2018
Massachusetts General Hospital
2010-2014
Harvard University
2005-2014
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the recent COVID-19 public health crisis. Bat is widely believed original host of SARS-CoV-2. However, its intermediate before transmitting to humans not clear. Some studies proposed pangolin, snake, or turtle as hosts. Angiotensin-converting enzyme (ACE2) receptor for SARS-CoV-2, which determines potential range On basis structural information complex human ACE2 and SARS-CoV-2 receptor-binding domain (RBD), we analyzed...
Responses to alpha interferon (IFN-α)-based treatment are dependent on both host and viral factors vary markedly among patients infected with different hepatitis C virus (HCV) genotypes (GTs). Patients GT3 viruses consistently respond better IFN than do GT1 viruses. The mechanisms underlying this difference not well understood. In study, we sought determine the effects of HCV NS5A proteins from signaling. We found that overexpression either or significantly inhibited IFN-induced...
Chikungunya virus (CHIKV) is transmitted to people by mosquitoes, and CHIKV infection causes fever joint pain. Fatty acid synthase (FASN) has been identified as a proviral factor for CHIKV. How FASN participates in replication remains be elucidated. In this study, we demonstrated that palmitic (PA) can restore the suppression of inhibitors. The palmitoylation plasma membrane localization nsP1 were reduced Triple mutation Cys417, Cys418, Cys419 blocked its severely disrupted replication....
Zika virus is a positive single-strand RNA whose replication involved unwinding and synthesis. ZIKV NS3 contains helicase domain, but its enzymatic activity not fully characterized. Here, we established dsRNA assay based on the FRET effect to study of NS3, which provided kinetic information in real time. We found that specifically unwound dsRNA/dsDNA with 3' overhang 5' direction. The ability significantly decreased when duplex was longer than 18 base pairs. depends ATP hydrolysis binding...
The global COVID-19 pandemic is caused by SARS-CoV-2, which one of seven human coronaviruses. G-quadruplexes are intrinsic obstacles to genome replication. Whether present in coronaviruses unknown. In the current study, we have predicted that all harbor G-quadruplex sequences. Conserved sequences SARS-CoV and SARS-CoV-2 were analyzed verified CD spectroscopy Thioflavin T fluorescence assay. Similar SARS-CoV, encodes an nsP3 protein, associate with G-quadruplexes. Targeting ligands could be a...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the cause of ongoing disease 2019 (COVID‐19) pandemic. The S protein key viral for associating with ACE2, receptor SARS‐CoV‐2. There are many kinds posttranslational modifications in protein. However, detailed mechanism palmitoylation SARS‐CoV‐2 remains to be elucidated. In our current study, we characterized S. Both C15 and cytoplasmic tail were palmitoylated. Fatty acid synthase inhibitor C75 zinc finger DHHC...
Tunneling nanotubes (TNTs) are actin-rich intercellular conduits that mediate distant cell-to-cell communication and enable the transfer of various cargos, including proteins, organelles, virions. They play vital roles in both physiological pathological processes. In this review, we focus on TNTs different types viruses, retroviruses such as HIV, HTLV, influenza A, herpesvirus, paramyxovirus, alphavirus SARS-CoV-2. We summarize viral proteins responsible for inducing TNT formation explore...
Myxoma virus (MYXV) M062R is a functional homolog of the C7L family host range genes from orthopoxviruses. We constructed targeted M062R-knockout-MYXV (vMyxM062-KO) and characterized its properties in vitro vivo. In European rabbits, infection by vMyxM062-KO was completely asymptomatic. The surviving rabbits did not gain full protection against subsequent lethal-dose challenge with wild-type MYXV. also looked for cellular tropism defects variety cultured cells. all rabbit cells tested,...
Cellular levels of phosphatidylinositol 4-phosphate (PI4P) have been shown to be upregulated during RNA replication several viruses, including the HCV replicon model. However, whether PI4P is required in an infectious model remains unknown. Moreover, it not established host transport machinery sequestered by generation infection. Here we found that was enriched complexes when Huh7.5.1 cells were infected with JFH1. inhibited upon overexpression phosphatase Sac1. The kinase PI4KIIIβ also for...
Abstract Viruses evolve multiple ways to interfere with NF-κB signaling, a key regulator of innate and adaptive immunity. Enterovirus 71 (EV71) is one primary pathogens that cause hand-foot-mouth disease. Here, we identify RelA(p65) as novel binding partner for EV71 2C protein from yeast two-hybrid screen. By interaction IPT domain p65, reduces the formation heterodimer p65/p50, predominant form NF-κB. We also show picornavirus family proteins inhibit activation associate p65 IKKβ. Our...
Corona Virus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) broke out in Wuhan city, Hubei province, China December 2019.1Li Q. Guan X. Wu P. Wang Zhou L. Tong Y. Ren R. Leung K.S.M. Lau E.H.Y. Wong J.Y. Xing Xiang N. Li C. Chen D. Liu T. Zhao J. M. Tu W. Jin Yang S. H. Luo Shao G. Tao Z. Deng B. Ma Zhang Shi Lam T.T.Y. J.T.K. Gao G.F. Cowling B.J. G.M. Feng Early transmission dynamics Wuhan, China, of Novel coronavirus-infected pneumonia.N Engl J Med....
Type I interferon (IFN) inhibits viruses by inducing the expression of antiviral proteins. The IFN-induced myxovirus resistance B (MxB) protein has been reported to inhibit a limited number viruses, including HIV-1 and herpesviruses, but its coverage remains be explored further. Here we show that MxB interferes with RNA replication hepatitis C virus (HCV) significantly viral in cyclophilin A (CypA)-dependent manner. Our data further interacts HCV NS5A, thereby impairing NS5A interaction CypA...