A5059832599- Mollusks and Parasites Studies
- Aquaculture Nutrition and Growth
- Environmental Toxicology and Ecotoxicology
- Mercury impact and mitigation studies
- Crustacean biology and ecology
- Invertebrate Immune Response Mechanisms
- Medical and Biological Ozone Research
- Therapeutic Uses of Natural Elements
- Lipid metabolism and biosynthesis
- Neurobiology and Insect Physiology Research
- Marine Bivalve and Aquaculture Studies
- Fatty Acid Research and Health
- Meat and Animal Product Quality
- Reproductive biology and impacts on aquatic species
- Paleopathology and ancient diseases
- Lipid Membrane Structure and Behavior
- Marine Biology and Environmental Chemistry
- Physiological and biochemical adaptations
- Vitamin K Research Studies
- Spaceflight effects on biology
- Selenium in Biological Systems
- Parasite Biology and Host Interactions
- Metabolomics and Mass Spectrometry Studies
- Protein Hydrolysis and Bioactive Peptides
- Invertebrate Taxonomy and Ecology
Biochemistry Research Institute of La Plata
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
Universidad Nacional de La Plata
2014-2025
Hong Kong Baptist University
2019
Hospital Virgen del Camino
2010
Centro Científico Tecnológico - La Plata
2006
Technical University of Nova Scotia
1992-1997
Apple snails (Ampullariidae) are among the largest and most ecologically important freshwater snails. The introduction of multiple species has reinvigorated field spurred a burgeoning body research since early 1990s, particularly regarding two introduced to Asian wetlands elsewhere, where they have become serious agricultural pests. This review places these recent advances in context previous work, across diverse fields ranging from phylogenetics biogeography through ecology developmental...
The family Ampullariidae includes both aquatic and amphibious apple snails. They are an emerging model for evolutionary studies due to the high diversity, ancient history, wide geographical distribution. Insight into drivers of ampullariid evolution is hampered, however, by lack genomic resources. Here, we report genomes four ampullariids spanning Old World (Lanistes nyassanus) New (Pomacea canaliculata, P. maculata, Marisa cornuarietis) clades. have conserved bilaterial karyotype features a...
The importance of the content anionic phospholipids [cardiolipin (CL) and phosphatidylglycerol (PG)] in osmotic adaptation membrane structure Bacillus subtilis cultures was investigated. Insertion mutations three putative cardiolipin synthase genes ( ywiE , ywnE ywjE ) were obtained. Only mutation resulted a complete deficiency thus corresponds to true clsA gene. osmotolerance mutant impaired: although at NaCl concentrations lower than 1·2 M growth curves similar those its wild-type control,...
Although most eggs are intensely predated, the aerial egg clutches from aquatic snail Pomacea canaliculata have only one reported predator due to unparalleled biochemical defenses. These include two storage-proteins: ovorubin that provides a conspicuous (presumably warning) coloration and has antinutritive antidigestive properties, PcPV2 neurotoxin with lethal effect on rodents. We sequenced studied whether it was able withstand gastrointestinal environment reach circulation of potential...
Fieldwork has thoroughly established that most eggs are intensely predated. Among the few exceptions aerial egg clutches from aquatic snail Pomacea canaliculata which have virtually no predators. Its defenses advertised by pigmented ovorubin perivitellin providing a conspicuous reddish coloration. The nature of defense however, was not clear, except for screening identified neurotoxic with lethal effect on rodents. Ovorubin is proteinase inhibitor (PI) whose role to protect against pathogens...
Energy partitioning within the developing eggs and early juveniles of gastropod Pomacea canaliculata was investigated from time fertilization to newly hatched snails. The forms locations energy stored as well their changes while development proceeded were studied in six stages (from morula 3-day hatchlings). rates utilization different lipid classes, protein total carbohydrates measured for first embryos perivitelline fluid. Perivitelline fluid is primary store. Its biochemical composition...
Pomacea canaliculata is a freshwater snail that deposits eggs on solid substrates above the water surface. Previous studies have emphasized nutritional and protective functions of three most abundant perivitelline fluid (PVF) protein complexes (ovorubin, PV2, PV3) during its embryonic development, but little known about structure function other less proteins. Using 2-DE, SDS-PAGE, MALDI TOF/TOF, LC–MS/MS, we identified 59 proteins from PVF P. canaliculata, among which 19 are novel. KEGG...
Gastropod hemocyanins are potent immunostimulants in mammals, a trait associated with their large molecular size and unusual glycosylation patterns. While the hemocyanin from marine snail keyhole limpet (KLH), has been widely studied successfully employed as carrier/adjuvant several immunological applications, well non-specific immunostimulant for bladder cancer treatment, few other gastropod have biochemically immunologically characterized. In this work, we investigated immunogenic...
The perivitellin-2 (PV2) from snails is an unusual neuro and enterotoxin comprising a pore-forming domain of the Membrane Attack Complex Perforin Family (MACPF) linked to lectin. While both domains have membrane binding capabilities, PV2’s mechanism action remains unclear. We studied apple snail Pomacea maculata (PmPV2’s) interaction with lipid membranes using various biophysical cell biology approaches. In vitro studies showed that PmPV2 toxicity decreased when cholesterol (Chol) was...
Abstract Energy partitioning, composition of lipids and fatty acids, their utilization by embryos were determined in the lecithotrophic shrimp Macrobrachium borellii during seven development stages. The biochemical at stage I is represented lipids, proteins, carbohydrates, with 29.3, 28.7, 0.2% dry weight, respectively. former two identified as major energy‐providing components, contributing 131 60 cal/100 mg egg, overall conversion efficiency (CE) was 45.0% (calculated percentage vitelline...
The freshwater snails Pomacea Perry, 1810 lay conspicuous aerial egg clutches that are ignored by most predators. Egg biochemical defenses in the apple snail canaliculata (Lamarck, 1822) provided multifunctional proteins. We analyzed eggs of a sympatric species, maculata 1810, studying gross composition, toxicity, hemagglutinating activity, and its antinutritive antidigestive properties. Eggs mostly composed polysaccharides (mainly galactogen) proteins, followed lipids nonsoluble calcium....
The lipid and protein composition of the perivitelline fluid eggs Pomacea canaliculata was investigated. Two lipoproteins (PV 1 PV 2) one lipoprotein fraction 3) were detected for first time in gastropods. They represent 57.0, 7.5, 35.5% egg total proteins, respectively. is a glyco-carotene-protein complex with characteristics very high-density (VHDL). It has 0.33% lipids, mainly free sterols phospholipids. particle MW 300 Kd composed three subunits 35, 32, 28 Kd, 2 VHDL 400 3.75% lipids....
Gastropoda, with approximately 80,000 living species, is the largest class of Mollusca. Among gastropods, apple snails (family Ampullariidae) are globally distributed in tropical and subtropical freshwater ecosystems many species ecologically economically important. Ampullariids exhibit various morphological physiological adaptations to their respective habitats, which make them ideal candidates for studying adaptation, population divergence, speciation, larger-scale patterns diversity,...
Background The spread of the invasive snail Pomacea canaliculata is expanding rat lungworm disease beyond its native range. Their toxic eggs have virtually no predators and unusual defenses including a neurotoxic lectin proteinase inhibitor, presumably advertised by warning coloration. We explored effect egg perivitellin fluid (PVF) ingestion on small intestine morphology physiology. Methodology/Principal Findings Through combination biochemical, histochemical, histopathological, scanning...