A5024682572- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Immune Cell Function and Interaction
- Toxin Mechanisms and Immunotoxins
- Signaling Pathways in Disease
- Biochemical and Molecular Research
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- Infant Nutrition and Health
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- Helicobacter pylori-related gastroenterology studies
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Polyamine Metabolism and Applications
- Microbial Metabolites in Food Biotechnology
- Diet, Metabolism, and Disease
- Lysosomal Storage Disorders Research
- Amino Acid Enzymes and Metabolism
- Celiac Disease Research and Management
- Polysaccharides and Plant Cell Walls
- Animal health and immunology
- Antifungal resistance and susceptibility
- Epigenetics and DNA Methylation
Experimental Medicine and Biology Institute
2014-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2025
Universidad Argentina de la Empresa
2024
Vrije Universiteit Amsterdam
2023
Universidad de Navarra
2023
National Institute for Bioprocessing Research and Training
2010-2014
BlackRock (United States)
2014
University of Dundee
2010-2013
Wellcome Trust
2013
University College Dublin
2010-2013
The present study was conducted to obtain a comprehensive overview of oligosaccharides in the milk variety important domestic animals including cows, goats, sheep, pigs, horses and dromedary camels. Using an analytical workflow that included ultra-performance liquid chromatography–hydrophilic interaction chromatography with fluorescence detection coupled quadrupole time-of-flight MS, detailed oligosaccharide libraries were established. partial or full characterisation neutral/fucosylated,...
Although the properties of milk oligosaccharides have been scientific interest for many years, their structural diversity presents a challenging analytical task. In quest simple and robust technology to characterize different present in milk, we developed an scheme based on fluorescent labeling, pre-fractionation by weak anionic exchange chromatography separation hydrophilic interaction liquid (HILIC)-high performance (HPLC). HILIC relies potential molecule, which accounts differences such...
Activation of AMP-activated protein kinase (AMPK) in endothelial cells regulates energy homeostasis, stress protection and angiogenesis, but the underlying mechanisms are incompletely understood. Using a label-free phosphoproteomic analysis, we identified glutamine:fructose-6-phosphate amidotransferase 1 (GFAT1) as an AMPK substrate. GFAT1 is rate-limiting enzyme hexosamine biosynthesis pathway (HBP) such controls modification proteins by O-linked β-N-acetylglucosamine (O-GlcNAc). In present...
Significance Although immune checkpoint blockade therapies have achieved long-term responses in several malignancies, colorectal cancer (CRC) patients clinical benefit is observed only heavily mutated tumors that are mismatch-repair–deficient or high microsatellite instability. This limitation urges the identification of novel escape mechanisms and design additional immunotherapeutic modalities. We show Galectin-1 (Gal-1) confers privilege to CRC by increasing frequency CD8 + CD122 PD-1...
Interleukin-12 (IL-12) gene transfer enhances the therapeutic potency of adoptive T cell therapies. We previously reported that transient engineering tumor-specific CD8 cells with IL-12 mRNA enhanced their systemic efficacy when delivered intratumorally. Here, we mix engineered mRNAs to express either single-chain (scIL-12) or an IL-18 decoy-resistant variant (DRIL18) is not functionally hampered by binding protein (IL-18BP). These mRNA-engineered mixtures are repeatedly injected into mouse...
Hypoxia, angiogenesis, and immunosuppression have been proposed to be interrelated events that fuel tumor progression impair the clinical effectiveness of anti-tumor therapies. Here we present new mechanistic data highlighting role hypoxia in fine-tuning CD8 T cell exhaustion vitro , an attempt reconcile seemingly opposite evidence regarding impact on functional features exhausted cells. Focusing recently characterized terminally-differentiated progenitor cells, found both its regulated...
Blockade of vascular endothelial growth factor (VEGF) signaling with bevacizumab, a humanized anti-VEGF monoclonal antibody (mAb), or receptor tyrosine kinase inhibitors, has improved progression-free survival and, in some indications, overall across several types cancers by interrupting tumor angiogenesis. However, the clinical benefit conferred these therapies is variable, and tumors from treated patients eventually reinitiate growth. Previously we demonstrated, mouse models, that...
Gastropod hemocyanins are potent immunostimulants in mammals, a trait associated with their large molecular size and unusual glycosylation patterns. While the hemocyanin from marine snail keyhole limpet (KLH), has been widely studied successfully employed as carrier/adjuvant several immunological applications, well non-specific immunostimulant for bladder cancer treatment, few other gastropod have biochemically immunologically characterized. In this work, we investigated immunogenic...
Glycosylation is the most common posttranslational modification of proteins and highly reflective changes in environment a cell. Epigenetic modifications to genome are stably transmitted daughter cells without requirement for genetic sequence alterations. Aberrant regulation both epigenetic programming glycosylation patterning integral aspects carcinogenesis. The objective this study was determine interplay between these two complex cellular processes. We demonstrate that global DNA...
ABSTRACT Helicobacter pylori and Campylobacter jejuni colonize the stomach intestinal mucus, respectively. Using a combination of mucus-secreting cells, purified mucins, novel mucin microarray platform, we examined interactions these two organisms with mucus mucins. H. C. bound to distinctly different displayed striking tropism for chicken gastrointestinal mucins compared from other animals preferentially specific avian sites (in order descending preference: large intestine, proximal small...
A main polysaccharide (aloe mannan) isolated from the fresh leaf pulp (aquiferous tissue) of Aloe arborescens Mill. var. natalensis Berger in a pure state was proved to be partially acetylated β–D–mannan. The molecular weight aloe mannan calculated approximately 15,000 by equilibrium ultracentrifugation. An inhibiting effect tested against implanted sarcoma–180.
Significance Chlamydia trachomatis ( Ct ) is the most common bacterium responsible for sexually transmitted infections. It constitutes a major public health burden, with greatest clinical impact occurring in women of reproductive age. A vast proportion infections are underestimated because they asymptomatic nature. This leads to chronic severe consequences, such as ectopic pregnancy, tubal obstruction, infertility, and blindness. an obligate intracellular pathogen that completes its entire...
Galectins, a family of animal lectins characterized by their affinity for N-acetyllactosamine-enriched glycoconjugates, modulate several immune cell processes shaping the course innate and adaptive responses. Through interaction with wide range glycosylated receptors bearing complex branched N-glycans core 2-O-glycans, these endogenous trigger distinct signaling programs thereby controling activation, differentiation, recruitment survival. Given unique features mucosal inflammation...
A gene predicted to encode Trypanosoma brucei glucosamine 6-phosphate N-acetyltransferase (TbGNA1; EC 2.3.1.4) was cloned and expressed in Escherichia coli. The recombinant protein enzymatically active, its high-resolution crystal structure obtained at 1.86 Å. Endogenous TbGNA1 localized the peroxisome-like microbody, glycosome. bloodstream-form T. GNA1 conditional null mutant constructed shown be unable sustain growth vitro under nonpermissive conditions, demonstrating that there are no...
The enzymes phosphomannomutase (PMM), phospho-N-acetylglucosamine mutase (PAGM) and phosphoglucomutase (PGM) reversibly catalyse the transfer of phosphate between C6 C1 hydroxyl groups mannose, N-acetylglucosamine glucose respectively. Although genes for a candidate PMM PAGM have been found in Trypanosoma brucei genome, there is, surprisingly, no gene PGM. TbPMM TbPAGM were cloned expressed Escherichia coli enzyme was crystallized its structure solved at 1.85 Å resolution. Antibodies to...
Aspergillus fumigatus is the causative agent of IA (invasive aspergillosis) in immunocompromised patients. It possesses a cell wall composed chitin, glucan and galactomannan, polymeric carbohydrates synthesized by processive glycosyltransferases from intracellular sugar nucleotide donors. Here we demonstrate that A. an active AfAGM1 (A. N-acetylphosphoglucosamine mutase), key enzyme biosynthesis UDP (uridine diphosphate)–GlcNAc (N-acetylglucosamine), donor for chitin synthesis. A conditional...