A5005175577- Trypanosoma species research and implications
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Malaria Research and Control
- Invertebrate Immune Response Mechanisms
- Microbial Inactivation Methods
- Research on Leishmaniasis Studies
- Diet, Metabolism, and Disease
- Health and Medical Education
- E-Learning and Knowledge Management
- Biochemical effects in animals
- 3D Printing in Biomedical Research
- Insect symbiosis and bacterial influences
- Respiratory viral infections research
- Hepatitis Viruses Studies and Epidemiology
- Ethics and bioethics in healthcare
- Synthesis and Biological Evaluation
- Amino Acid Enzymes and Metabolism
- Hemoglobin structure and function
- Blood transfusion and management
- MRI in cancer diagnosis
- Erythropoietin and Anemia Treatment
- Electrohydrodynamics and Fluid Dynamics
- SARS-CoV-2 detection and testing
- Microfluidic and Bio-sensing Technologies
European Molecular Biology Laboratory
2024-2025
Hospital Clínic de Barcelona
2020
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2020
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2020
Barcelona Institute for Global Health
2016-2019
Universitat de Barcelona
2013-2019
Johns Hopkins University
2019
University of Florida
2019
Universidad de Alcalá
1992-1999
Human serum albumin (HSA) is an emerging treatment for preventing excessive systemic inflammation and organ failure(s) in patients with acutely decompensated (AD) cirrhosis. Here, we investigated the molecular mechanisms underlying immunomodulatory properties of HSA. Administration HSA to AD cirrhosis elevated circulating bacterial DNA rich unmethylated cytosine-phosphate-guanine dideoxynucleotide motifs (CpG-DNA) was associated reduced plasma cytokine concentrations. In isolated leukocytes,...
Abstract In the mammalian host, Trypanosoma congolense cytoadheres, or sequesters, to vascular endothelium. Although sequestration influences clinical outcome, disease severity and organ pathology, its determinants mediators remain unknown. Challenges such as variability of animal models, only-recently developed tools genetically manipulate parasite, lack physiologically-relevant in vitro models have hindered progress. Here, we engineered brain cardiac 3D bovine endothelial microvessel that...
The disappearance of lytic, protective antibodies (Abs) from the serum patients with Chagas disease is accepted as a reliable indicator parasitological cure. efficiency chemiluminescent enzyme-linked immunosorbent assay based on purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for serologic detection lytic Abs against Trypanosoma cruzi was evaluated in nonendemic setting using panel 92 positive and 58 negative human sera. technique proved to be highly...
Abstract Glycosylation is an important posttranslational protein modification in all eukaryotes. Besides glycosylphosphatidylinositol (GPI) anchors and N -glycosylation, O -fucosylation has been recently reported key sporozoite proteins of the malaria parasite. Previous analyses showed presence GDP-fucose (GDP-Fuc), precursor for fucosylation reactions, blood stages Plasmodium falciparum . The GDP-Fuc de novo pathway, which requires action GDP-mannose 4,6-dehydratase (GMD) GDP-L-fucose...
Cerebral malaria is a severe neurovascular complication of Plasmodium falciparum with high mortality, even after treatment effective antimalarials. A better understanding pathogenic mechanisms could help future development adjunctive therapies, yet limitations in current experimental models have hindered our knowledge the disease. We developed 3D blood-brain barrier model enhanced properties using primary brain endothelial cells, astrocytes and pericytes. Exposure to parasite egress products...
Thrombospondin type I repeat (TSR) domains are commonly O-fucosylated by protein O-fucosyltransferase 2 (PoFUT2), and this modification is required for optimal folding secretion of TSR-containing proteins. The human malaria parasite Plasmodium falciparum expresses proteins containing TSR domains, such as the thrombospondin-related anonymous (TRAP) circumsporozoite surface (CSP), which O-fucosylated. TRAP CSP present on sporozoites play essential roles in mosquito host invasion processes...
Glutamate dehydrogenase (GDH) and alcohol (ADH) have been encapsulated in sheep human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At fixed enzyme level the dialysis bag (100 units/ml of RBCs), significant encapsulation yield was observed for ADH, both (17.2%) (47.9%) RBCs, whereas very low entrapment GDH achieved (1-3%) either species. Carrier cell recovery 61-65% humans 30-34% sheep. Because aggregation to large polymers at protein levels above 1 mg/ml,...