A5041336677- Galectins and Cancer Biology
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Glycosylation and Glycoproteins Research
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Health Literacy and Information Accessibility
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- COVID-19 Clinical Research Studies
- Mobile Health and mHealth Applications
- vaccines and immunoinformatics approaches
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Inflammasome and immune disorders
- Fibroblast Growth Factor Research
- RNA Research and Splicing
- Lung Cancer Research Studies
- Toxin Mechanisms and Immunotoxins
Experimental Medicine and Biology Institute
2019-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2019-2024
Universidad Argentina de la Empresa
2023-2024
Instituto de Desarrollo Tecnológico para la Industria Química
2023-2024
Vrije Universiteit Amsterdam
2023
ORCID
2021
Lewisham and Greenwich NHS Trust
2015
Queen Elizabeth Hospital
2015
Although immune checkpoint blockers have yielded significant clinical benefits in patients with different malignancies, the efficacy of these therapies is still limited. Here, we show that disruption transmembrane protein 176B (TMEM176B) contributes to CD8+ T cell-mediated tumor growth inhibition by unleashing inflammasome activation. Lack Tmem176b enhances antitumor activity anti-CTLA-4 antibodies through mechanisms involving caspase-1/IL-1β Accordingly, responding blockade display an...
Highlights•We used 3 tools to evaluate the quality of internet information for breast cancer.•46% websites were classed as 'excellent' according DISCERN criteria.•9 out 26 found be HONcode certified.•7 complied with all four JAMA benchmarks.•Breast cancer online was generally good; however range wide.AbstractThe is frequently by patients researching regarding cancer. This study aims assess these using validated tools.The term 'breast cancer' searched in search engines. The top 20 results...
Blockade of vascular endothelial growth factor (VEGF) signaling with bevacizumab, a humanized anti-VEGF monoclonal antibody (mAb), or receptor tyrosine kinase inhibitors, has improved progression-free survival and, in some indications, overall across several types cancers by interrupting tumor angiogenesis. However, the clinical benefit conferred these therapies is variable, and tumors from treated patients eventually reinitiate growth. Previously we demonstrated, mouse models, that...
Abstract Background Programmed death ligand‐1 (PD‐L1) expression is a well‐known predictive biomarker of response to immune checkpoint blockade in non‐small cell lung cancer (NSCLC). However, there limited evidence the relationship between PD‐L1 expression, clinicopathological features, and their association with major driver mutations NSCLC patients Latin America. Methods This retrospective study included from Argentina advanced NSCLC, centralized evaluation concurrently genomic alterations...
The accurate quantification of tumor-infiltrating immune cells turns crucial to uncover their role in tumor escape, determine patient prognosis and predict response checkpoint blockade. Current state-of-the-art methods that quantify from biopsies using gene expression data apply computational deconvolution present multicollinearity estimation errors resulting the overestimation or underestimation diversity infiltrating quantity. To overcome such limitations, we developed MIXTURE, a new...
Abstract RNA sequencing has proved to be an efficient high-throughput technique robustly characterize the presence and quantity of in tumor biopsies at a given time. Importantly, it can used computationally estimate composition immune infiltrate infer immunological phenotypes those cells. Given significant impact anti-cancer immunotherapies role associated microenvironment (ITME) on its prognosis therapy response, estimation cell-type content is crucial for designing effective strategies...
The role of the molecular tumour board (MTB) is to recommend personalised therapy for patients with cancer beyond standard-of-care treatment. A comprehensive analysis in a pathology laboratory important all targeted therapies approaches. Here we report 1-year experience Instituto Alexander Fleming Molecular Tumour Board.The MTB was launched December 2019 monthly meeting. In each interactive session, five cases were presented and discussed by members. These referred treating oncologists....
e15154 Background: The analysis of somatic genomic alterations, evaluation DNA mismatch repair (MMR) proteins, and examination PD-L1 expression in tumor biopsies are crucial for prognosis informed therapeutic decisions. However, their clinical implementation Argentina faces challenges due to economic costs lack evidence. To address this, we conducted a retrospective study assess the impact this molecular panel testing on treatment decisions management cancer patients Argentina. Additionally,...
Abstract Overcoming luminal breast cancer (BrCa) progression remains a critical challenge for improved overall patient survival. RUNX2 has emerged as protein related to aggressiveness in triple‐negative BrCa, however its role tumors elusive. We have previously shown that active FGFR2 (FGFR2‐CA) contributes increased tumor growth and expression was high hormone‐independent mouse mammary carcinomas. To elucidate the interaction between human we investigated their roles treatment...
The internet is frequently used by patients for researching information regarding breast cancer. This study aims to assess the quality of these websites using validated tools. term 'breast cancer' was searched in 3 search engines. top 20 results were selected, and duplicates irrelevant excluded. 26/34 analysed DISCERN Plus tool, HONcode JAMA benchmarks. 46% classed as 'excellent' when assessed with tool. range scores wide (range: 25-74). Nine found be certified. Seven complied all four shows...
e15198 Background: Immunotherapies have revolutionized cancer treatment, but responses are not universal and patients who initially respond to therapy develop resistance. The accurate quantification of tumor-infiltrating immune cells holds the promise reveal role system in human cancers its involvement tumor escape mechanisms response therapy. We present MIXTURE, a new algorithm for cell-type proportions deconvolution from transcriptomic data that overcomes competitive methods revealed novel...
Abstract Both targeted therapies (TT) and immunotherapies have revolutionized the treatment of metastatic melanoma patients. Particularly, TT with BRAFi or BRAFi/MEKi leads to prompt but not durable responses in most patients due development resistance within 1 year. Tumors refractory derive little benefit from subsequent immunotherapy. Here we aim elucidate how previous treatments shape tumor-immune landscape it could be overcome therapeutically. Firstly, analyzed gene expression profile a...
Abstract Although the development of single-cell RNAseq (scRNAseq) has improved knowledge tumor microenvironment (TME), there is not a clear understanding specific cell subtypes, markers that define these populations, nor interaction between them. Previously, we showed inflammasome activation correlated with antitumor responses triggered by immune checkpoint blockade (ICB). This highlights central role for TMEM176B, mostly expressed in myeloid cells, impairs recruitment cytotoxic cells TME...
e21155 Background: KRAS is mutated in ∼30% of non-small-cell lung cancer (NSCLC). Most the mutations involve codon 12 exon 2, with G12C mutation being most frequent (~12%). Differences mutational frequency have been demonstrated patients different ethnicities and smoking status. However, effects ancestry environmental factors not exhaustively described for Argentine population. Methods: An observational study was conducted a cohort 1810 patients. Specifically, 1510 retrospective tissue...
Abstract Aberrant glycosylation has emerged as an important hallmark of cancer playing a critical role in the modulation tumor cell aggressiveness, invasiveness, and immune vascular programs. However, cellular molecular mechanisms implicated glycan-driven progression remain poorly understood. In this study, we explored expression profile glycosylation-related gene signature (Affymetrix, GlycoV4) its association with tumors from different primary sites. First, analyzed published...
Abstract Cell surface glycans play essential roles in diverse physiological and pathological processes their assessment has important implications biomedicine biotechnology. Here we present a rapid, versatile, single-step multicolor flow cytometry method for evaluation of cell glycan signatures using panel selected fluorochrome-conjugated lectins. This procedure allows simultaneous detection with 10-fold reduction the number cells required compared traditional multistep lectin staining...
<h3>Background</h3> Although immunotherapies have emerged as efficient strategies for treating cancer, biomarkers outcome prediction are still under debate. Relevant such PD-L1, tumor mutational burden, and microsatellite instability (MSI) shown limited efficacy stand-alone markers.<sup>1 2</sup> Moreover, aberrant glycosylation has been associated to development, but its potential predict immunotherapy outcomes barely explored.<sup>3</sup> We studied the expression of glyco-immune genes...
Endogenous lectins play key roles in cell homeostasis by decoding the information encrypted glycans present on surface or extracellular matrix. Galectins, a family of soluble lectins, have emerged as central regulators innate and adaptive immune responses. In this article, we review seminal work demonstrating immunoregulatory Galectin-1 (Gal-1), proto-type member galectin family, highlight mechanisms that control its functions cancer autoimmune inflammation. Understanding cellular pathways...
Abstract Most melanoma patients who develop resistance to targeted therapies do not respond subsequent immunotherapy. Therefore, establishing which would derive clinical benefit from immunotherapy is a compelling question. We performed meta-analysis of published transcriptome datasets biopsies before and after development BRAFi or BRAFi/MEKi. observed an increase in M2-macrophages infiltrate by CIBERSORT relapsed as well T-cell exhaustion markers immunosuppressive cytokines. Our preliminary...
Abstract Glioblastomas (GBM), the most prevalent and lethal primary brain tumors, are characterized by high intertumoral heterogeneity, diffuse infiltration, resistance to conventional therapies. Notably, ability of tumor cells invade surrounding tissues is one their damaging characteristics, it not only causes therapies such as surgery radiotherapy but ultimately cause death. Therapies that hypoxia (e.g. anti-angiogenic therapies) have been shown increase invasiveness, leading therapy...