A5085385226- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Cell death mechanisms and regulation
- RNA Research and Splicing
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Biochemical and Molecular Research
- Endoplasmic Reticulum Stress and Disease
- Porphyrin Metabolism and Disorders
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Cancer therapeutics and mechanisms
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Phagocytosis and Immune Regulation
- Advanced Breast Cancer Therapies
- Mesenchymal stem cell research
- Folate and B Vitamins Research
Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
Fundación Ciencias Exactas y Naturales
2001-2014
University of Buenos Aires
1998-2014
The etiology of Parkinson's disease (PD) converges on a common pathogenic pathway mitochondrial defects in which α-Synuclein (αSyn) is thought to play role. However, the mechanisms by αSyn and its disease-associated allelic variants cause dysfunction remain unknown. Here, we analyzed axonal transport morphology human-derived neurons overexpressing wild-type (WT) or mutated A30P A53T, are known have differential lipid affinities. A53T was enriched fractions, inducing significant...
Abstract Human embryonic and induced pluripotent stem cells are self-renewing (PSC) that can differentiate into a wide range of specialized cells. Basic fibroblast growth factor is essential for PSC survival, stemness self-renewal. PI3K/AKT pathway regulates cell viability apoptosis in many types. Although it has been demonstrated activation by bFGF relevant maintenance its role on survival remains elusive. In this study we explored the molecular mechanisms involved regulation AKT. We found...
Mesenchymal stem cells (MSCs) are a promising source of for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as suitable alternative MSC production. Nevertheless, at present, PD-MSC derivation protocols either expensive or not clinical purposes.In this work we present therapy-grade, inexpensive and simple protocol to derive (PSCs)...
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as pluripotency both vivo vitro, normoxia hypoxia. Embryonic Stem Cell (hESC) WA-09 reprogrammed fibroblast primary HFF-FM controls.
Background A central aspect of development and disease is the control cell proliferation through regulation mitotic cycle. Cell cycle progression directionality requires an appropriate balance positive negative regulators whose expression must fluctuate in a coordinated manner. p19INK4d, member INK4 family CDK inhibitors, has unique feature that distinguishes it from remaining makes likely candidate for contributing to p19INK4d mRNA protein levels accumulate periodically during under normal...
Although BMP4-induced differentiation of glioma stem cells (GSCs) is well recognized, details the cellular responses triggered by this morphogen are still poorly defined. In study, we established several GSC-enriched cell lines (GSC-ECLs) from high-grade gliomas. The expansion these as adherent monolayers, and not floating neurospheres, enabled a thorough study phenotypic changes that occurred during their differentiation. Herein, evaluated GSC-ECLs' behavior toward differentiating...
Embryonic stem cells (ESCs) need to maintain their genomic integrity in response DNA damage safeguard the of organism. double strand breaks (DSBs) are one most lethal forms and, if not repaired correctly, they can lead cell death, instability and cancer. How human ESCs (hESCs) agents that cause DSBs is relatively unclear. In present study we aim determine hESC DSB inducing agent camptothecin (CPT). We find hESCs hypersensitive CPT, as evidenced by high levels apoptosis. CPT treatment leads...
MicroRNAs are small non-coding RNAs involved in post-transcriptional regulation of gene expression related to many cellular functions. We performed a small-RNAseq analysis cardiac differentiation from pluripotent stem cells. Our analyses identified some new aspects about microRNA this process. First, we described dynamic profile microRNAs where them clustered according their level. Second, the extensive network isomiRs and ADAR modifications. Third, families clusters establishment lineage...
Human embryonic stem cells (hESCs) are self-renewing pluripotent that can differentiate into specialized and hold great promise as models for human development disease studies, cell-replacement therapies, drug discovery in vitro cytotoxicity tests. The culture differentiation of these both complex expensive, so it is essential to extreme aseptic conditions. hESCs susceptible Mycoplasma sp. infection, which hard detect alters cell-associated properties. purpose this work was evaluate the...
High-grade gliomas are the most prevalent and lethal primary brain tumors. They display a hierarchical arrangement with population of self-renewing highly tumorigenic cells called cancer stem cells. These thought to be responsible for tumor recurrence, which make them main candidates targeted therapies. Unbridled cell cycle progression may explain selective sensitivity some treatments. The members Cip/Kip family p21Cip1 p27Kip1 were initially considered as suppressors based on their ability...
The transcription factor SOX11 plays an important role in embryonic neurogenesis and tissue remodeling. Recent studies have shown aberrant expression of various types aggressive B cell neoplasms. In this study, we analyzed levels 86 patients with diagnosis chronic lymphocytic leukemia (CLL). Results were correlated well-known prognostic factors such as immunoglobulin heavy chain variable (IGHV) gene mutational status, cytogenetics risk groups clinicopathological characteristics the disease....
Abstract Human embryonic and induced pluripotent stem cells are self-renewing (hPSCs) that can differentiate into a wide range of specialized cells. Although moderate hypoxia (5% O 2 ) improves hPSC self-renewal, pluripotency, cell survival, the effect acute severe (1% on viability is still not fully elucidated. In this sense, we explore consequences survival by culturing them under (maximum 24 h) physical ). After h hypoxia, observed HIF-1α stabilization concomitant with decrease in...
Although the negative regulation of gene expression by insulin has been widely studied, transcription factors responsible for effect are still unknown. The purpose this work was to explore molecular mechanisms involved in repression 5-aminolevulinate synthase (ALAS) gene. Deletion analysis 5′-regulatory region allowed us identify an insulin-responsive located at –459 –354 bp. This fragment contains a highly homologous (IRE) sequence. By transient transfection assays, we determined that...
Activation protein-1 (AP-1) transcription factors are early response genes involved in a diverse set of transcriptional regulatory processes. The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is often used to induce AP-1 activity. purpose this work was explore the molecular mechanisms TPA regulation ubiquitous 5-aminolevulinate synthase (ALAS) gene expression, first and rate-controlling step heme biosynthesis. Previous analysis 5′-flanking sequence ALAS revealed existence two...
Human pluripotent stem cells (hPSCs), including embryonic and induced (hESCs hiPSCs) show unique cell cycle characteristics, such as a short doubling time due to an abbreviated G1 phase. Whether or not the core machinery directly regulates stemness and/or differentiation potential of hPSCs remains be determined. To date, several scenarios describing atypical have been suggested, therefore there is still controversy over how cyclins, master regulators cycle, are expressed regulated....
Human embryonic stem cells (hESCs) are hypersensitive to genotoxic stress and display lower survival ability relative their differentiated progeny. Herein, we attempted investigate the source of this difference by comparing DNA damage responses triggered topoisomerase I inhibitor camptothecin, in hESCs, human induced pluripotent (hiPSCs) hESCs-derived neuroprogenitors (NP). We observed that upon camptothecin exposure underwent apoptosis more swiftly at a higher rate than cells. However,...