A5048617242- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- COVID-19 Clinical Research Studies
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Renal and related cancers
- Sperm and Testicular Function
- Whipple's Disease and Interleukins
- MicroRNA in disease regulation
- Muscle Physiology and Disorders
- Cardiomyopathy and Myosin Studies
- Cell Image Analysis Techniques
- Tissue Engineering and Regenerative Medicine
- Long-Term Effects of COVID-19
- COVID-19 and healthcare impacts
- Chromosomal and Genetic Variations
- Respiratory Support and Mechanisms
- Virus-based gene therapy research
- Image Processing Techniques and Applications
- FOXO transcription factor regulation
- COVID-19 Impact on Reproduction
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Reproductive System and Pregnancy
Ospedale Sacro Cuore Don Calabria
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2024
Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
2016-2024
Instituto de Neurología Cognitiva
2021-2024
University of Oxford
2021
Physiologie de la Reproduction et des Comportements
2016-2021
Centro Científico Tecnológico - San Juan
2021
King's College London
2019
University of Buenos Aires
2010-2018
Although antibody levels progressively decrease following SARS-CoV-2 infection, the immune memory persists for months. Thus, individuals who naturally contracted are expected to develop a more rapid and sustained response COVID-19 vaccines than naïve individuals. In this study, we analyzed dynamics of BNT162b2 mRNA vaccine in six healthcare workers March 2020, comparison nine control subjects without previous infection. The was well tolerated by both groups, with no significant difference...
Deep learning is a significant step forward for developing autonomous tasks. One of its branches, computer vision, allows image recognition with high accuracy thanks to the use convolutional neural networks (CNNs). Our goal was train CNN transmitted light microscopy images distinguish pluripotent stem cells from early differentiating cells. We induced differentiation mouse embryonic epiblast-like and took at several time points initial stimulus. found that can be trained recognize...
Abstract Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis it also implicated in several physiological pathological events including stem cell differentiation. During early stages of differentiation, human cells pass through EMT where deeper morphological, molecular biochemical changes occur. Though initially considered as decision between two states, now regarded fluid exist on...
Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced large quantities stored until ready use. Although the has already been proven, thorough...
Abstract The analysis of structural and functional neuroimaging data using graph theory has increasingly become a popular approach for visualising understanding anatomical relationships between different cerebral areas. In this work we applied network-based brain PET studies population-based covariance matrices, with the aim to explore topological tracer kinetic differences in cross-sectional investigations. Simulations, test-retest applications datasets from three tracers ([ 18 F]FDG, [...
To assess the antibody response to BNT162b2 mRNA COVID-19 vaccine in a cohort of health-care workers (HCW), comparing individuals with previous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and SARS-CoV-2-naive individuals.HCW were tested at T0 (day first dose), T1 second dose) T2 (2-3 weeks after for IgG anti-nucleocapsid protein, IgM anti-spike protein anti-receptor binding domain (IgG-RBD-S). The was compared between four main groups: group A, positive antibodies...
Understanding the cause of sex disparities in COVID-19 outcomes is a major challenge. We investigate hormone levels and their association with patients, stratified by age. This observational, retrospective, cohort study included 138 patients aged 18 years or older COVID-19, hospitalized Italy between February 1 May 30, 2020. The hormones (testosterone, estradiol, progesterone, dehydroepiandrosterone) (ARDS, severe in-hospital mortality) was explored 120 50 over. STROBE checklist followed....
The aim of this study was to evaluate the capacity domestic cat (Dc, Felis silvestris) oocytes reprogram nucleus cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), using embryo aggregation. Dc were in vitro matured and subjected zona pellucida free (ZP-free) or iSCNT, depending on whether donor cell Ch respectively. ZP-free reconstructed embryos then cultured microwells individually (Dc1X Ch1X groups) couples (Dc2X Ch2X groups). Embryo aggregation improved development...
Abstract The application of new technologies for gene editing in horses may allow the generation improved sportive individuals. Here, we aimed to knock out myostatin ( MSTN ), a negative regulator muscle mass development, using CRISPR/Cas9 and generate edited embryos first time horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg different gRNA/Cas9 plasmids targeting exon . observed that increasing plasmid concentrations mutation efficiency. average efficiency was 63.6% gRNA1...
The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency still very low. To address this issue, we first evaluated the effects of time from cell fusion activation (<1h, n = 1261; 1-2h, 1773; 2-3h, 1647) on in vitro and vivo development embryos generated by cloning. Then, using different donor types two successive experiments: I) induced pluripotent stem cells (iPSCs) vs. adult fibroblasts (AF) fused ooplasts injected...
Abstract Background International travellers frequently acquire infectious diseases whilst travelling, yet relatively little is known about the impact and economic burden of these illnesses on travellers. We conducted a prospective exploratory costing study adult returning with falciparum malaria, dengue, chikungunya or Zika virus. Methods Patients were recruited in eight Travel Tropical Medicine clinics between June 2016 March 2020 upon travellers’ first contact health system their country...
Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) donors cloning, compared vitro vivo embryo development respect fibroblasts.Zona-free transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF n=4527). Embryos produced by...
In this study, we analyzed the effects of cloned embryo aggregation on in vitro development and quality by measuring blastocyst diameter cell number, DNA fragmentation levels expression genes associated with pluripotency, apoptosis, trophoblast methylation porcine. Zona-free reconstructed embryos were cultured well system, placing one (1x non aggregated group) or three (3x per microwell. Our results showed that increased formation rate pig embryos. 3x blastocysts significantly decreased...
Loiasis, caused by the filarial nematode Loa loa, is endemic in Central and West Africa where about 10 million people are infected. There a scarcity of convenient, commercial diagnostics for L. loa. Microscopy requires trained personnel has low sensitivity, while serodiagnosis currently not standardized. Individual case management also important non-endemic countries to treat migrants, expatriates tourists. We retrospectively compared performance Antibody Rapid Test (RDT) ELISA pan-filarial...
In the mouse embryo, transition from preimplantation to postimplantation epiblast is governed by changes in gene regulatory network (GRN) that lead transcriptional, epigenetic, and functional changes. This can be faithfully recapitulated vitro differentiation of embryonic stem cells (mESCs) epiblast-like (EpiLCs), reside naïve formative states pluripotency, respectively. However, GRN drives this conversion not fully elucidated. Here we demonstrate transcription factor OCT6 a key driver...
MicroRNAs are small non-coding RNAs involved in post-transcriptional regulation of gene expression related to many cellular functions. We performed a small-RNAseq analysis cardiac differentiation from pluripotent stem cells. Our analyses identified some new aspects about microRNA this process. First, we described dynamic profile microRNAs where them clustered according their level. Second, the extensive network isomiRs and ADAR modifications. Third, families clusters establishment lineage...
PIWI-interacting RNAs (piRNAs) are a class of non-coding initially thought to be restricted exclusively germline cells. In recent years, accumulating evidence has demonstrated that piRNAs actually expressed in pluripotent, neural, cardiac and even cancer However, controversy remains around the existence function somatic piRNAs. Using small RNA-seq samples from H9 pluripotent cells differentiated mesoderm progenitors cardiomyocytes we identified expression 447 piRNA transcripts, which 241...
Cell death experiments are routinely done in many labs around the world, these backbone of assays for drug development. detection is usually performed ways, and requires time reagents. However, cell preceded by slight morphological changes shape texture. In this paper, we trained a neural network to classify cells undergoing death. We found that was able highly predict after one hour exposure camptothecin. Moreover, prediction largely outperforms human ability. Finally, provide simple python...
Abstract The stem cell niche has a strong influence in the differentiation potential of human pluripotent cells with integrins playing major role communicating extracellular environment. However, it is not well understood how interactions between and matrix are involved cardiac differentiation. To evaluate this, we performed profile expression two stages differentiation: mesodermal progenitors cardiomyocytes. We found an active regulation different during In particular, integrin α 5 subunit...
Background: The estimation of Plasmodium falciparum parasitaemia can vary according to the method used. Recently, droplet digital PCR (ddPCR) has been proposed as a promising approach in molecular quantitation Plasmodium, but its ability predict actual on clinical samples not largely investigated. Moreover, possibility applying ddPCR-sensitive serum never explored. Methods: We used, for first time, ddPCR both blood and detect DNA P. 52 paired from 26 patients. was compared with loop-mediated...
Cancer immunotherapies based mainly on the blockade of immune-checkpoint (IC) molecules by anti-IC antibodies offer new alternatives for treatment in oncological diseases. However, a considerable proportion patients remain unresponsive to them. Hence, development novel clinical immunotherapeutic approaches and/or targets are crucial.W In this context, targeting HLA-G/ILT2/ILT4 has caused great interest since it is abnormally expressed several malignancies generating tolerogenic...