A5000681523- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Reproductive Physiology in Livestock
- Molecular Biology Techniques and Applications
- Ovarian function and disorders
- Pregnancy and preeclampsia studies
- Reproductive Health and Technologies
- Renal and related cancers
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Veterinary Equine Medical Research
- Xenotransplantation and immune response
- Circular RNAs in diseases
- Urological Disorders and Treatments
- Reproductive biology and impacts on aquatic species
- Hormonal and reproductive studies
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Identification and Quantification in Food
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Advanced Proteomics Techniques and Applications
University of Zurich
2018-2024
University Hospital of Zurich
2024
Physiologie de la Reproduction et des Comportements
2015-2022
Université de Tours
2016-2022
Centre National de la Recherche Scientifique
2016-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2022
Centre Val de Loire
2019
University of Sheffield
2012-2017
Ghent University
2016
Universidad de Murcia
2004-2015
Successful pregnancy requires an appropriate communication between the mother and embryo. Recently, exosomes microvesicles, both membrane-bound extracellular vesicles (EVs) present in oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known about their content role during oviduct-embryo dialog. Given differences secretions by vivo vitro epithelial cells (OEC), we aimed at deciphering EVs protein from sources. Moreover, analyzed functional effect...
The success of early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players mediating this crucial cross-talk by transferring their cargo (proteins, mRNA small ncRNA) from cell to cell. However, little is known about EVs (oEVS) composition implications success. aim study was determine oEVs content at protein, RNA level examine whether under hormonal...
Sex allocation of offspring in mammals is usually considered as a matter chance, being dependent on whether an X- or Y-chromosome-bearing spermatozoon reaches the oocyte first. Here we investigated alternative possibility, namely that oviducts can recognise and Y- spermatozoa, may thus be able to bias sex ratio. By introducing Y-sperm populations into two separate single female pigs using bilateral laparoscopic insemination found spermatozoa did indeed elicit sex-specific transcriptomic...
The maternal tract plays a critical role in the success of early embryonic development providing an optimal environment for establishment and maintenance pregnancy. Preparation this requires intimate dialogue between embryo her mother. However, many intriguing aspects remain unknown unique communication system. To advance our understanding process by which blastocyst is accepted endometrium better address clinical challenges infertility pregnancy failure, it imperative to decipher complex...
High polyspermy is one of the major limitations porcine invitro fertilisation (IVF). The addition oviductal fluid (OF) during IVF reduces without decreasing rate. Because extracellular vesicles (EVs) have been described as important OF components, aim this study was to evaluate effect EVs (poEVs) on efficiency compared with (fresh and lyophilised). collected from abattoir oviducts by phosphate-buffered saline flush, poEVs were isolated serial ultracentrifugation. Four treatments conducted:...
Oviductal extracellular vesicles (oEVs) are emerging as key players in the gamete/embryo-oviduct interactions that contribute to successful pregnancy. Various positive effects of oEVs on gametes and early embryos have been found vitro. To determine whether these associated with changes embryonic gene expression, transcriptomes supplemented bovine fresh (FeEVs) or frozen (FoEVs) during vitro culture compared controls without were analyzed by low-input RNA sequencing. Analysis RNA-seq data...
Follicular fluid (FF) fills the interior portion of ovarian antral follicle and provides a suitable microenvironment for growth enclosed oocyte, through molecular factors that originate from plasma secretions follicular cells. FF contains extracellular nanovesicles (ffEVs), including 30–100 nm membrane-coated exosomes, which carry different types RNA, proteins lipids directly influence oocyte competence to develop embryo. In present study, we aimed i) characterize protein cargo EVs 3–6 mm...
Oviductal extracellular vesicles (oEVs) have been identified as important components of the oviductal fluid (OF) and pointed to key modulators gamete/embryo-maternal interactions. Here, we determined functional impact oEVs on embryo development embryonic transcriptome in porcine. Experiment 1 examined effect OF development. In vitro-produced embryos were cultured with or for 2 7 days using an vitro sequential system without supplementation (control). analyzed transcriptomic alterations...
Study question: Could follicular fluid-derived extracellular vesicles (ffEVs) benefit human oocyte rescue in vitro maturation (rIVM)? Summary answer: Supplementation of rIVM culture with ffEVs isolated from mature follicles enhanced rates by >20%, inducing changes protein profile and organelle distribution. What is already known: IVM involves the immature germinal vesicle (GV) oocytes under set laboratory conditions to allow for their transition metaphase II (MII) stage, which confirmed...
Summary PSP‐I/PSP‐II heterodimer is a major protein of boar seminal plasma that able to preserve, in vitro, the viability, motility and mitochondrial activity highly‐extended spermatozoa. However, relationship between protective effects sperm capacitation still unclear. The present study investigated effect (1.5 mg/mL) on membrane stability, intracellular calcium concentration ([Ca 2+ ] I ) acrosome integrity highly extended Boar spermatozoa were diluted 1 × 10 6 spermatozoa/mL incubated at...
The constitutive 70 kDa heat-shock protein, HSPA8, has previously been shown to contribute the long-term survival of spermatozoa inside mammalian female reproductive tract. Here, we show that a recombinant form HSPA8 rapidly promotes viability uncapacitated spermatozoa, ability bind oviductal epithelial cells, enhances IVF performance, and decreases sperm mitochondrial activity. Fluorescence recovery after photobleaching revealed repair membrane damage is achieved by an almost instantaneous...
Oviductal extracellular vesicles (oEVs) have been proposed as key modulators of gamete/embryo maternal interactions. The aim this study was to examine the metabolite content oEVs and its regulation across estrous cycle in cattle. EVs were isolated from bovine oviducts ipsilateral contralateral ovulation at four stages (post-ovulatory stage, early late luteal phases, pre-ovulatory stage). metabolomic profiling performed by proton nuclear magnetic resonance spectroscopy (NMR). NMR identified...
Understanding the composition of oviduct fluid (OF) is crucial to better comprehend microenvironment in which sperm capacitation, fertilization and early embryo development take place. Therefore, our aim was determine spatiotemporal changes OF proteome according anatomical region (ampulla vs. isthmus), proximity ovulating ovary (ipsilateral contralateral side) peri-ovulatory stage (pre-ovulatory or Pre-ov post-ovulatory Post-ov). Oviducts from adult cyclic cows were collected at a local...
In vitro, efficient communication between mammalian embryos in groups or and cocultured somatic cells implies that there is a sender, message receiver able to decode the message. Embryos secrete variety of autocrine paracrine factors and, these, extracellular vesicles have recently been implicated as putative messengers embryo-embryo communication, well embryo with maternal tract. Extracellular (EVs) are membrane-bound found biofluids culture media conditioned by presence cells. EVs carry...
Syncytins are endogenous retroviral envelope proteins which induce the fusion of membranes. A human representative this group, retrovirus group W member 1 (ERVW-1) or syncytin-1 is present in trophoblast-derived extracellular vesicles and supports incorporation these into recipient cells. During pregnancy, placenta-derived participate feto-maternal communication. Bovine fetal binucleate trophoblast cells express syncytin, bovine protein K1 (BERV-K1). These release maternal stroma, but it...
Extracellular vesicles (EVs) have been identified in the uterine fluid different species and pointed as key players embryo-maternal dialogue, maternal recognition of pregnancy establishment pregnancy. However, little is known about EVs mare. Therefore, present study aimed at characterizing from lavage cyclic mares by comparing five isolation methods combination them: (1) ultracentrifugation (UC); (2) concentration volume Centricon ultrafiltration (CE); (3) use CE with washing steps...
In the present study, effects of retinoid metabolite administration during in vitro maturation (IVM) on oocyte maturation, parameters fertilisation (IVF) and embryo development were examined. Varying concentrations 9-cis retinoic acid (RA; 0, 5, 50 500 nm; Experiment 1) all-trans retinol (ROH; 125, 1250 12 2) included medium. Cumulus–oocyte complexes matured inseminated with frozen–thawed spermatozoa. Presumptive zygotes cultured for 16 h to assess IVF or 7 days quality. 1, rate metaphase II...