Centrosome formation during early development in mice and rats occurs due to the appearance of centrioles de novo. In contrast, humans other non-rodent mammals, are thought be derived from spermatozoa. Ultrastructural study zygotes embryos cattle at full series ultrathin sections show that proximal centriole spermatozoon disappears by end first cleavage division. Centrioles appear two four cell fertilized oocytes parthenogenetic embryos. Centriole includes atypical with randomly arranged...

In vitro embryo production (IVP) using oocytes obtained through transvaginal follicular aspiration (ovum pick up, OPU) is a widely recognized tool for breeding and preserving cattle breeds. The aim of this study was to assess the influence individual donor factors on efficiency OPU/IVP technique in Istoben breed dependency OPU frequency. procedure performed mature heifers (n=7) regimen 2 (2W) 1 (1W) times per week. Each underwent 8-9 consecutive sessions each compared factor influenced...

The aim of this study was to evaluate the impact frequency electrofusion on efficiency sheep cloning and assess dependence effect duration serum starvation somatic cells (SC). Materials Methods . Fetal fibroblasts at passages IV-V were used as donor for cloning. To arrest SCs in G0/G1 phase cell cycle, after reaching near-confluence (approximately 90%), subjected (SS) 24 48 hours. Recipient oocytes collected from post-mortem ovaries, matured vitro, reconstructed through enucleation followed...

Follicular fluid (FF) fills the interior portion of ovarian antral follicle and provides a suitable microenvironment for growth enclosed oocyte, through molecular factors that originate from plasma secretions follicular cells. FF contains extracellular nanovesicles (ffEVs), including 30–100 nm membrane-coated exosomes, which carry different types RNA, proteins lipids directly influence oocyte competence to develop embryo. In present study, we aimed i) characterize protein cargo EVs 3–6 mm...

Lipid metabolism in ovarian follicular cells supports the preparation of an enclosed oocyte to ovulation. We aimed compare lipid composition a dominant large follicle (LF) and subordinated small follicles (SFs) within same ovaries. Mass spectrometry imaging displayed differences distribution several features between different follicles. Comparison fingerprints LF SF by Matrix Assisted Laser Desorption/Ionisation Time-Of-Flight (MALDI-TOF) mass revealed that oocytes, only 8 out 468 detected...

Aging processes accelerate dramatically in oocytes that have reached the metaphase-II (M-II) stage. The present work aimed to study patterns and intracellular pathways of actions prolactin (PRL) growth hormone (GH) on age-associated changes bovine M-II aging vitro. To this end, we analyzed spontaneous parthenogenetic activation (cytogenetic assay), apoptosis (TUNEL developmental capacity (IVF/IVC) vitro-matured after prolonged culturing. Both PRL GH reduced rate cumulus-enclosed (CEOs)...

Extinction of many species is irreversible and a part the natural evolution, but human activities have influenced this process, making it much faster comparing to speciation.According FAO, approximately 20 % breeds cattle, goats, pigs, horses poultry in world are currently at risk disappearance, died past few years, as result their genetic characteristics lost forever.The role banks management resources conservation endangered particularly noticeable last decade.Most cryobanks focus on...

In vitro maturation is an important process in the production of embryos. It has been shown that three cytokines, fibroblast growth factor 2, leukemia inhibitory and insulin-like 1 (FLI), increased efficiency maturation, somatic cell nuclear transfer (SCNT) blastocyst production, vivo development genetically engineered piglets.Assess effects FLI on oocyte quality oocytes, embryo bovine fertilisation (IVF) SCNT.Cytokine supplementation resulted significant increases rates decreased levels...

Follicular fluid (FF) ensures a safe environment for oocyte growth and maturation inside the ovarian follicle in mammals. In each cycle, large dominant (LF) contains designated to be ovulated, whereas small subordinate follicles (SFs) of same wave will die through atresia. cows, oocytes from SF, being 2 mm size, are suitable vitro reproduction biotechnologies, their competence developing an embryo depends on size follicles. FF proteins, metabolites, fatty acids, multitude extracellular...

РЕЗУЛЬТАТЫ ПОЛУЧЕНИЯ И ТРАСПЛАНТАЦИИ IVEP ЭМБРИОНОВ У ОВЕЦ (Ovis aries) Г.Н.СИНГИНА  , В

General senescence of the adult organism is closely connected with reproductive one. Meanwhile, age-related reduction in female fertility primarily associated a decline gamete quality. Molecular and cellular changes oocytes old mammalian females are very similar to those occurring during aging matured ova their young counterparts, suggesting similarities underlying mechanisms. The aim present work was study actions two related pituitary hormones, prolactin (PRL) growth hormone (GH), on...

Reproductive health is an important trait in selection of dairy cattle. A genome-wide association study (GWAS) a powerful tool for annotating phenotypic effects on the genome and to get knowledge genes chromosomal regions associated with reproductive performance (Cole et al. 2011). Combining GWAS genetic profiling embryos before implantation enables develop new strategies select elite breeding genotypes transfer (Humblot 2010; Ponsart 2013). The aim current was evaluate between single...

Summary Centrosome formation during early development in mice and rats occurs due to the appearance of centrioles de novo . In contrast, humans other non-rodent mammals, are thought be derived from spermatozoa. Ultrastructural study zygotes embryos cattle at full series ultrathin sections showed that proximal centriole spermatozoon disappears by end first cleavage division. Centrioles appear 2–4 cell fertilized oocytes parthenogenetic embryos. Centriole includes atypical with randomly...

Abstract Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that calf identical fibroblast cell line for SCNT. CRISPR/Cas9 approach subsequently knock out genes beta-lactoglobulin gene (PAEP) and beta-lactoglobulin-like protein ( LOC100848610) cells. Gene editing (GE) efficiency 4.4% each of these genes. We successfully obtained single-cell-derived colonies containing PAEP LOC100848610...

Protein palmitoylation is a reversible post-translational modification by fatty acids (FA), mainly palmitate (C16:0). Palmitoylation allows protein shuttling between the plasma membrane and cytosol to regulate stability, sorting signaling activity its deficiency leads diseases. We aimed characterize palmitoyl-proteome of ovarian follicular cells molecular machinery regulating within follicle. For first time, 84 palmitoylated proteins were identified from bovine granulosa (GC), cumulus (CC)...

Extinction of many species is irreversible and a part the natural evolution, but human activities have influenced this process, making it much faster comparing to speciation. According FAO, approximately 20 % breeds cattle, goats, pigs, horses poultry in world are currently at risk disappearance, died past few years, as result their genetic characteristics lost forever. The role banks management resources conservation endangered particularly noticeable last decade. Most cryobanks focus on...

СМЕНА СРЕДЫ КУЛЬТИВИРОВАНИЯ ПОЛОЖИТЕЛЬНО ВЛИЯЕТ НА РАЗВИТИЕ И КАЧЕСТВО ЭМБРИОНОВ КРУПНОГО РОГАТОГО СКОТА in vitro Г.Н

В статье рассмотрено влияние различной интенсивности сессий на результативность прижизненной УЗИ-ассистированной пункции фолликулов у коров (OPU). Исследования проводили телках-донорах симментальской породы (n=4). Была выполнена 91 сессия OPU с интервалами между ними 3, 4, 7 и 8 дней. Наибольшая в отношении числа визуализированных наблюдалась при проведении их аспирации интервалом 3 дня. среднем за сессию было аспирировано 15,85 фолликула, что, соответственно, 5,44 (P≤0,001) 5,08 фолликула...