A5041944975- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- Genetic and Kidney Cyst Diseases
- Sperm and Testicular Function
- Receptor Mechanisms and Signaling
- Protist diversity and phylogeny
- Neuropeptides and Animal Physiology
- Protein Kinase Regulation and GTPase Signaling
- Chromosomal and Genetic Variations
- Reproductive Health and Technologies
- Neuroscience and Neuropharmacology Research
- Renal and related cancers
- Cannabis and Cannabinoid Research
- Neurotransmitter Receptor Influence on Behavior
- Micro and Nano Robotics
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- scientometrics and bibliometrics research
- Cell Adhesion Molecules Research
- Reproductive Physiology in Livestock
- Invertebrate Immune Response Mechanisms
University of Toledo
2016-2025
National Institutes of Health
2000-2023
Servy Institute for Reproductive Endocrinology
2023
Harvard University
2008-2020
Google (United States)
2017
Hybrigenics (France)
2014
University of California, San Diego
2009
Howard Hughes Medical Institute
2004-2009
Weizmann Institute of Science
1994-2005
National Institute of Diabetes and Digestive and Kidney Diseases
2000
Two cannabinoid receptors, designated neuronal (or CB1) and peripheral CB2), have recently been cloned. Activation of CB1 receptors leads to inhibition adenylate cyclase N‐type voltage‐dependent Ca 2+ channels. Here we show, using a CB2 transfected Chinese hamster ovary cell line, that this receptor binds variety tricyclic ligands as well the endogenous ligand anandamide. by various cannabinoids inhibits activity is pertussis toxin sensitive indicating coupled G i /G 0 GTP‐binding proteins....
Abstract The centriole is the core structure of centrosome and cilium. Failure to restrict duplication once per cell cycle has serious consequences commonly observed in cancer. Despite its medical importance, mechanism formation poorly understood. Asl was previously reported be a centrosomal protein essential for function. Here we identify mecD, severe loss-of-function allele asl gene, demonstrate that it required cilia formation. Similarly, Cep152, ortholog vertebrates, function can...
Most animals have two centrioles in spermatids (the distal and proximal centrioles), but insect seem to contain only one centriole (Fuller 1993), which functionally resembles the centriole. Using fluorescent centriolar markers, we identified a structure near fly that is reminiscent of (i.e., centriole-like, or PCL). We show PCL exhibits several features daughter centrioles. First, single forms segment older Second, proteins SAS-6, Ana1, Bld10p/Cep135 are PCL. Third, formation depends on...
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that sperm contains, in addition to known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical (distal DC) composed splayed microtubules previously undescribed rods luminal proteins. is remodeled by both reduction enrichment specific proteins formation these spermatogenesis. In vivo vitro investigations flagellum-attached,...
Abstract Reproductive success depends on efficient sperm movement driven by axonemal dynein-mediated microtubule sliding. Models predict sliding at the base of tail – centriole but such has never been observed. Centrioles are ancient organelles with a conserved architecture; their rigidity is thought to restrict Here, we show that, in mammalian sperm, atypical distal (DC) and its surrounding pericentriolar matrix form dynamic basal complex (DBC) that facilitates cascade internal...
Abstract Centrioles are subcellular organelles found at the cilia base with an evolutionarily conserved structure and a shock absorber-like function. In sperm, centrioles flagellum essential for embryo development in basal animals. Yet, sperm have evolved diverse forms, sometimes acting like transmission system, as cattle, becoming dispensable, house mice. How centriole to become dispensable some organisms is unclear. Here, we test hypothesis that this transition occurred through cascade of...
While acute activation of inhibitory Gi/o-coupled receptors leads to inhibition adenylyl cyclase, chronic such an increase in cAMP accumulation. This phenomenon, observed many cell types, has been referred as cyclase superactivation. At this stage, the mechanism leading superactivation and nature isozyme(s) responsible for phenomenon are largely unknown. Here we show that transfection isozymes into COS-7 cells results isozyme-specific AC activity upon stimulation (e.g. with forskolin,...
Using CHO cells stably transfected with rat μ-opioid receptor cDNA, we show that the μ-agonists morphine and [D-Ala2, N-methyl-Phe4,Gly-ol5]enkephalin are negatively coupled to adenylylcyclase inhibit forskolin-stimulated cAMP accumulation. Chronic exposure of leads rapid development tolerance. Withdrawal or following chronic treatment (by wash addition antagonist naloxone) an immediate increase in cyclase activity (supersensitization overshoot), which is gradually reversed upon further...
Two cannabinoid receptors belonging to the superfamily of G protein-coupled membrane have been identified and cloned: neuronal receptor (CB1) peripheral (CB2). They shown couple directly G(i/o) subclass proteins mediate inhibition adenylyl cyclase upon binding a agonist. In several cases, however, cannabinoids reported stimulate activity, although mechanism by which they did so was unclear. With cloning nine isozymes with various properties, including different sensitivities alpha(s),...
(-)-Δ9-Tetrahydrocannabinol ((-)-Δ9-THC) is the major active psychotropic component of marijuana plant, Cannabis sativa. The membrane proteins that have been found to bind this material or its derivatives called cannabinoid receptors. Two GTP-binding protein-coupled receptors cloned. CB1 neuronal receptor mostly in cells and tissues while CB2 peripheral has detected spleen several immune system. It previously shown activation by agonists inhibits adenylyl cyclase activity. Utilizing Chinese...
Centrioles are subcellular organelles essential for normal cell function and development; they form the cell's centrosome (a major cytoplasmic microtubule organization center) cilium sensory motile hair-like cellular extension). with evolutionarily conserved characteristics found in most animal types but absent egg cells exhibit unexpectedly high structural, compositional, functional diversity sperm cells. As a result, centriole's precise role fertility early embryo development is unclear....
Abstract: Although it is well‐established that G protein‐coupled receptor signaling systems can network with those of tyrosine kinase receptors by several mechanisms, the point(s) convergence two pathways remains largely undelineated, particularly for opioids. Here we demonstrate opioid agonists modulate activity extracellular signal‐regulated protein (ERK) in African green monkey kidney COS‐7 cells transiently cotransfected μ‐, δ‐, or κ‐opioid and ERK1‐ ERK2‐containing plasmids. Recombinant...