A5074078747- Genetic and Kidney Cyst Diseases
- Cystic Fibrosis Research Advances
- Protist diversity and phylogeny
- Neonatal Respiratory Health Research
- Microtubule and mitosis dynamics
- Sperm and Testicular Function
- Axon Guidance and Neuronal Signaling
- Infant Health and Development
- Micro and Nano Robotics
- Pediatric health and respiratory diseases
- Reproductive Biology and Fertility
- Atmospheric chemistry and aerosols
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Toxic Organic Pollutants Impact
- Plant Reproductive Biology
- T-cell and B-cell Immunology
- Calpain Protease Function and Regulation
- Acute Myeloid Leukemia Research
- Respiratory viral infections research
- Per- and polyfluoroalkyl substances research
- Platelet Disorders and Treatments
- Fetal and Pediatric Neurological Disorders
- Lipid metabolism and biosynthesis
- Complement system in diseases
- Wireless Body Area Networks
Simmons University
2025
University of Toronto
2018-2020
Hospital for Sick Children
2017-2020
SickKids Foundation
2018
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that sperm contains, in addition to known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical (distal DC) composed splayed microtubules previously undescribed rods luminal proteins. is remodeled by both reduction enrichment specific proteins formation these spermatogenesis. In vivo vitro investigations flagellum-attached,...
Abstract Background Perfluoroalkyl substances (PFAS) are persistent environmental contaminants previously used for industrial purposes as a non‐stick coating and flame retardant. The stability of these molecules prevents their breakdown, which results in ground water contamination across the globe. known to bioaccumulate various organisms. However, health consequences remain unclear due large number PFAS family different effects on tissues. Here, we use frog Xenopus laevis investigate...
Quantitative super-resolution imaging improves the diagnosis of primary ciliary dyskinesia.
Abstract Motile cilia are beating machines that play a critical role in airway defense. During cell differentiation, hundreds of motile templated from basal bodies extend foot, an appendage links together to ensure coordination. This assembly has thus far escaped structural analysis because its size is below the resolution limit. Here, we determine molecular architecture and identify foot proteins using super-resolution-driven approach. Quantitative super-resolution image shows organized...
Abstract Our current understanding of phagocytosis is largely derived from studies individual receptor‐ligand interactions and their downstream signaling pathways. Because phagocytes are exposed to a variety ligands on heterogeneous target particles in vivo, it important observe the engagement multiple receptors simultaneously triggered involvement Potential crosstalk between two well‐characterized opsonic receptors, FcγR CR3, was briefly explored early 1970s, where macrophages were...
Abstract This document presents detailed steps to analysing the waveforms of beating cilia, measured in high speed video microscopy. We show that case PCD caused by mutations DNAH11 and HYDIN classification a handful parameters describing ciliary dynamics allows distinguish genotype, as well (much more easily) distinguishing healthy from samples. is intended complement brief highlight (1) details datasets used letter.
Primary ciliary dyskinesia (PCD) is a heterogeneous recessive genetic disease characterized by impaired mucociliary clearance. PCD currently diagnosed analysis of transmission electron microscopy micrographs or genome sequencing biallelic pathogenic variants in one 37 known genes. Despite recent advances the genetics PCD, there are suspected cases that remain unresolved. <b>Methods:</b> We used immunofluorescence together with 3DSIM super-resolution (SRM) on airway epithelial cells freshly...
Corticospinal neurons project to various subcortical targets including the basilar pons via axon collateral branches.Previously, we showed that produces diffusible chemoattractant promote branch initiation.However, molecular mechanism underlying collateralization is still unclear.To identify molecules are essential for collateralization, performed knockdown experiments receptors expressed in corticospinal neurons.We found of one receptor protein tyrosine phosphatases (RPTPs), tentatively...