A5103187026- Congenital heart defects research
- Pluripotent Stem Cells Research
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Acute Myeloid Leukemia Research
- Pediatric Hepatobiliary Diseases and Treatments
- Cystic Fibrosis Research Advances
- Trace Elements in Health
- Ion channel regulation and function
- Structural Behavior of Reinforced Concrete
- Hematological disorders and diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Concrete Corrosion and Durability
- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Wind and Air Flow Studies
- Hematopoietic Stem Cell Transplantation
- Building Energy and Comfort Optimization
- Nerve injury and regeneration
- Respiratory viral infections research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Urban Heat Island Mitigation
Memorial Sloan Kettering Cancer Center
2025
Hebei University of Technology
2023
University of Toronto
2018-2022
University Health Network
2020-2022
Stem Cell Network
2021
Simon Fraser University
2018
Toronto Metropolitan University
2018
Hospital for Sick Children
2016-2018
Abstract Compact cardiomyocytes that make up the ventricular wall of adult heart represent an important therapeutic target population for modeling and treating cardiovascular diseases. Here, we established a differentiation strategy promotes specification, proliferation maturation compact from human pluripotent stem cells (hPSCs). The generated under these conditions display ability to use fatty acids as energy source, high mitochondrial mass, well-defined sarcomere structures enhanced...
While clinical trials are ongoing using human pluripotent stem cell-derived midbrain dopamine (mDA) neuron precursor grafts in Parkinson's disease (PD), current protocols to derive mDA neurons remain suboptimal. In particular, the yield of TH+ after vivo grafting and expression some subtype-specific markers can be further improved. For example, characterization by single cell transcriptomics has yielded only a small proportion considerable fraction contaminating populations. Here we present...
In the mouse, first hematopoietic cells are generated in yolk sac from primitive, erythro-myeloid progenitor (EMP) and lymphoid programs that specified before emergence of stem cells. While many sac-derived populations transient, specific immune cell progeny seed developing tissues, where they function into adult life. To access human equivalent these lineages, we modeled development using pluripotent differentiation. Here, show combination Activin A, BMP4, FGF2 induces a population...
The derivation of mature functional cholangiocytes from human pluripotent stem cells (hPSCs) provides a model for studying the pathogenesis cholangiopathies and developing therapies to treat them. Current differentiation protocols are not efficient give rise that fully mature, limiting their therapeutic applications. Here, we generate hPSC-derived display many characteristics bile duct including high levels cystic fibrosis transmembrane conductance regulator (CFTR) presence primary cilia...
Deletion of phenylalanine at position 508 (F508del) in cystic fibrosis transmembrane conductance regulator (CFTR) is the most common (CF)-causing mutation. Recently, ORKAMBI, a combination therapy that includes corrector processing defect F508del-CFTR (lumacaftor or VX-809) and potentiator channel activity (ivacaftor VX-770), was approved for CF patients homozygous this However, clinical studies revealed effect ORKAMBI on lung function modest it proposed relates to negative impact VX-770...
Cystic fibrosis transmembrane conductance regulator (CFTR) is a multidomain membrane protein that functions as phosphorylation-regulated anion channel. The interface between its two cytosolic nucleotide binding domains and coupling helices conferred by intracellular loops extending from the channel pore has been referred to transmission thought be critical for regulated activity of CFTR. Phosphorylation regulatory domain CFTR kinase A (PKA) required activity. However, it was unclear if...
Abstract The derivation of mature functional cholangiocytes from human pluripotent stem cells (hPSCs) would provide a model for studying the pathogenesis cholangiopathies and developing novel therapies to treat them. Current differentiation protocols are not efficient give rise that fully mature, limiting their therapeutic applications. Here, we describe new strategy generate hPSC-derived display many characteristics bile duct including high levels CFTR presence primary cilia capable sensing...