A5073710580- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Barrier Structure and Function Studies
- Connexins and lens biology
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Neuroscience and Neuropharmacology Research
- Wnt/β-catenin signaling in development and cancer
- Cellular transport and secretion
- Protist diversity and phylogeny
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Sperm and Testicular Function
- Advanced Fluorescence Microscopy Techniques
- Genetics, Aging, and Longevity in Model Organisms
- Chronic Myeloid Leukemia Treatments
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Micro and Nano Robotics
- 3D Printing in Biomedical Research
- Toxin Mechanisms and Immunotoxins
- Photoreceptor and optogenetics research
- Reproductive biology and impacts on aquatic species
- Cancer Genomics and Diagnostics
- Retinal Development and Disorders
University of Michigan
2015-2025
University of Iowa Hospitals and Clinics
2023-2024
Institut thématique Génétique, génomique et bioinformatique
2023
University of Iowa
2005-2020
University of Wisconsin–Madison
2005-2012
Madison Group (United States)
2012
British Infection Association
2012
Columbia University
2010
Institute of Molecular Biology and Biophysics
2004-2007
Yale University
2001-2003
Animal cells decide where to build the cytokinetic apparatus by sensing position of mitotic spindle. Reflecting a long-standing presumption that furrow-inducing stimulus travels from spindle cortex via microtubules, debate continues about which and in what geometry, are essential for accurate cytokinesis. We used live imaging urchin frog embryos evaluate relationship between microtubule organization furrow position. In normal cells, forms region lower cortical density. Remarkably, depleted...
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that sperm contains, in addition to known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical (distal DC) composed splayed microtubules previously undescribed rods luminal proteins. is remodeled by both reduction enrichment specific proteins formation these spermatogenesis. In vivo vitro investigations flagellum-attached,...
Epithelial cell–cell junctions remodel in response to mechanical stimuli maintain barrier function. Previously, we found that local leaks tight (TJs) are rapidly repaired by local, transient RhoA activation, termed “Rho flares,” but how Rho flares regulated is unknown. Here, discovered intracellular calcium flashes and junction elongation early events the flare pathway. Both laser-induced naturally occurring TJ breaks lead at site of leaks. Additionally, induced optogenetics increases...
Increased levels of macrolide-resistant Streptococcus pyogenes in focal regions the United States have been reported. The purpose this study was to determine antimicrobial susceptibility a large collection S. isolates from throughout and elucidate mechanisms resistance genetic relatedness isolates.During 2002-2003, total 1885 clinical were obtained 45 US medical centers. Susceptibility penicillin, cefdinir, erythromycin, azithromycin, clarithromycin, clindamycin, telithromycin, levofloxacin...
Objective Disturbances in the basal ganglia portions of cortico-striato-thalamo-cortical circuits likely contribute to symptoms attention deficit hyperactivity disorder (ADHD). The authors examined morphologic features nuclei (caudate, putamen, and globus pallidus) children with ADHD. Method A total 104 individuals (combined-type ADHD patients: N=47; healthy comparison subjects: N=57), aged 7 18 years, were a cross-sectional case-control study using anatomical magnetic resonance imaging....
Abl family nonreceptor tyrosine kinases regulate cellular morphogenesis and motility through functional interactions with the actin cytoskeleton. Although are known to contain filamentous (F)-actin-binding domains at their C termini, it is unclear how structure and/or function of We show here that Abl-related kinase Arg binds positive cooperativity F-actin in vitro binding saturating a ratio one Arg/two molecules. Measurements F-actin-binding properties deletion mutants led identification...
Microtubules (MTs) help establish and maintain cell polarity by promoting actin-dependent membrane protrusion at the leading edge of cell, but molecular mechanisms that mediate cross-talk between actin MTs during this process are unclear. We demonstrate Abl-related gene (Arg) nonreceptor tyrosine kinase is required for dynamic lamellipodial protrusions after adhesion to fibronectin. arg−/− fibroblasts exhibit reduced dynamics as compared with wild-type fibroblasts, defect can be rescued...
In migrating cells, actin polymerization promotes protrusion of the leading edge, whereas actomyosin contractility powers net cell body translocation. Although they promote F-actin-dependent protrusions periphery upon adhesion to fibronectin (FN), Abl family kinases inhibit migration on FN. We provide evidence here that Abl-related gene (Arg/Abl2) kinase inhibits fibroblast by attenuating and regulating focal dynamics. arg-/- fibroblasts migrate at faster average speeds than wild-type (wt)...
Abstract Surface monolayer techniques have been applied to the study of lipoprotein lipase from lipid-free bovine milk. Lipoprotein activity was determined by measurement decrease in surface radioactivity as a result enzymic hydrolysis glyceryl tri[1-14C]octanoate monolayer. The rate enhanced 5-fold 10 nm apolipoprotein glutamic acid human very low density lipoprotein. activator protein and were shown associate stable film absence lipid.
A phosphorylated derivative of streptomycin accumulated in cultures Streptomyces griseus ATCC 12475 and SC2376 grown on complex media containing an excess inorganic phosphate (0.01 m ). This compound did not accumulate significantly the absence added phosphate. The inhibit growth Bacillus subtilis or support a streptomycin-dependent strain Escherichia coli; however, incubation with alkaline phosphatase gave which was active both systems. In derivative, is esterified —OH group streptidine...