A5078727105- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Renal and related cancers
- Assisted Reproductive Technology and Twin Pregnancy
- CRISPR and Genetic Engineering
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Genetic diversity and population structure
- Retinoids in leukemia and cellular processes
- Genetic Mapping and Diversity in Plants and Animals
- Molecular Biology Techniques and Applications
- Birth, Development, and Health
- Tissue Engineering and Regenerative Medicine
- Ruminant Nutrition and Digestive Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant tissue culture and regeneration
- Agroforestry and silvopastoral systems
- Mesenchymal stem cell research
- Seed Germination and Physiology
CSIRO Oceans and Atmosphere
2025
Servicio Regional de Investigación y Desarrollo Agroalimentario
2014-2024
California State University, Fullerton
2024
IVI Murcia Clinic
1999-2023
Instituto de Biotecnología de León
2016-2017
Valencian Infertility Institute
2001-2014
Massachusetts Institute of Technology
2009
Institute for Animal Reproduction
2009
In-Q-Tel
2008
Instituto Tecnológico Agrario de Castilla y León
2008
Exposure of human spermatozoa to nicotinamide adenine dinucleotide phosphate (NADPH) resulted in the dose dependent generation reactive oxygen species (ROS) which, at a critical level intensity, induced lipid peroxidation, DNA damage and dramatic decline sperm motility. This system was then used as model for screening ability different antioxidants combat oxidative stress created through excessive intracellular toxic products metabolism. A variety that has previously been shown be protective...
Summary The aim of this work is to highlight the need monitoring small populations conserve their genetic variability by using a set parameters characterize both structure and management practices. As representative example we analyse pedigree information endangered Xalda sheep breed Asturias. herdbook included total 805 animals 62 herds. number founders was 329. Nowadays, there are 562 live 26 active in risk losing diversity because abusive use certain individuals as parents. effective...
We analyzed embryo-maternal interactions in the bovine uterus on day 8 of development. Proteomic profiles were obtained by two-dimensional difference gel electrophoresis from paired samples uterine fluid (UF) same animal with and without embryos uterus. Results contrasted UF after artificial insemination. detected 50 differential protein spots (t test, p < 0.05). Subsequent characterization nano-LC-ESI-MS/MS enabled us to identify 38 proteins, obtaining for first time earliest evidence...
Although many research papers have studied diversity and differentiation within livestock species, genetic relationships among neighboring populations remain poorly understood. Here we apply recent methodologies to analyze the polymorphism of 14 microsatellites in 238 unrelated individuals belonging six sheep breeds from Northern Spain ascertain their historical relative contributions existing between populations. Individual genotypes were analyzed assess existence an underlying structure....
Abstract Aurantiochytrium not only excels in producing long-chain polyunsaturated fatty acids such as docosahexaenoic acid for humans, but it is also a source of essential with minimal impacts on wild fisheries and vital the transfer atmospheric carbon to oceanic sinks cycles. This study aims unveil systems biology lipid production Australian sp. TC20 by comparing transcriptomic profiles under optimal growth conditions increased from early (Day 1) late exponential phase 3). Particular...
We used metadata to explore the metabolic interplay between culture medium from in vitro-produced bovine embryos transferred fresh or frozen, recipient blood plasma, and calf fitness, alongside gene expression methylation lymphocytes. Principal component (PC) analysis (PCA) identified covariates that were depicted Debiased Sparse Partial Correlation networks analyzed as enriched pathways. Four PCs explained 13.77, 9.58, 7.73 5.84% variability. PC1 clustered only mother weight two embryonic...
Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, ploidy in vitro produced bovine embryos. Subsequently, using real-time PCR, the expression genes expected to underlie observed at blastocyst stage. matured oocytes were either fertilized or activated with ionomycin +6-DMAP cultured simple medium....
Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls critical to normalise results, thereby avoiding differences and maximizing sensitivity accuracy. So far, many genes have been used as single reference gene, without having previously verified their value controls. This practice can lead incorrect conclusions recent evidence indicates need use geometric mean data from several...
Recent investigations have pointed to a high prevalence of Y chromosome submicroscopic deletions in men with severely impaired spermatogenesis. We report on the incidence 128 infertile men, whom karyotype, sperm count, and hormonal parameters were evaluated. Patients abnormal karyotype (other than an chromosome) or concentration more 2 million/mL excluded. Genomic DNA was extracted from peripheral leukocytes 57 azoospermia 71 severe oligospermia. Molecular analysis performed by 3 multiplex...
Despite a number of recent studies that have focused on the origin domestic horses, genetic relationships between major geographical clusters still remain poorly understood. In this study we analyzed 296 bp mtDNA fragment from HVI region 171 horses representing 11 native Iberian, Barb, and Exmoor breeds to assess maternal phylogeography Iberian horses. The haplogroup with CCG motif (nucleotide position 15,494 15,496) was most frequent in Barb (0.42 0.57, respectively), regardless geographic...
Results of this work show, in the inferior frontal gyrus and different portions into which it was divided, asymmetry weight cortical surface left predominance posterior portion, is concerned with language. In spite difference among absolute individual values, correlation between values excellent all cases, ratio same for related to language remainder gyrus.
The bovine endometrium recognizes early embryos and reacts differently depending on the developmental potential of embryo. However, it is unknown whether can distinguish embryonic sex. Our objective was to analyze sexual dimorphism in uterus response male female embryos. Differentially expressed (DE) proteins, different levels hexoses, other embryotrophic differences were analyzed uterine fluid (UF). Proteomic analysis day-8 UF recovered from heifers after transfer day-5 or identified 23 DE...
Retinoids have an important role in cell growth, morphogenesis and differentiation. In the present study developmental potential of bovine oocytes was examined after vitro maturation presence 9-cis-retinoic acid, a vitamin A metabolite, at 5 nmol l(-1) chemically defined conditions. Experiments studied early development, blastocyst differential counts capacity embryos to establish pregnancy transfer recipients. After fertilization culture simple medium, development hatching rates increased...
Recent studies have proposed the use of molecular coancestry coefficients as a measure genetic variability and useful tool for conservation purposes. Using simulated data, has been shown to become constant very quickly after separation populations, leading population diversity remaining constant. However, information study relationships between breeds not yet widely explored. Here we analyze polymorphism 14 microsatellites in 222 unrelated individuals belonging seven native Spanish ascertain...