A5035272186- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Pluripotent Stem Cells Research
- Renal and related cancers
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Genetic and phenotypic traits in livestock
- Seed Germination and Physiology
- Retinoids in leukemia and cellular processes
- Plant tissue culture and regeneration
- Molecular Biology Techniques and Applications
- Milk Quality and Mastitis in Dairy Cows
- Xenotransplantation and immune response
- Liver physiology and pathology
- Prenatal Screening and Diagnostics
- Ubiquitin and proteasome pathways
- Kruppel-like factors research
- Mesenchymal stem cell research
- Chromosomal and Genetic Variations
- Urological Disorders and Treatments
- Nutrition, Genetics, and Disease
Servicio Regional de Investigación y Desarrollo Agroalimentario
2011-2024
Centro Regional de Selección y Reproducción Animal
2024
Institute for Animal Reproduction
2009
University of Missouri
2003-2004
Iowa State University
1996-1999
The ubiquitin-proteasome pathway has been implicated in the penetration of ascidian vitelline envelope by fertilizing spermatozoon (Sawada et al., Proc Natl Acad Sci U S A 2002; 99:1223-1228). present study provides experimental evidence demonstrating proteasome involvement mammalian zona pellucida (ZP). Using porcine vitro fertilization as a model, ZP was completely inhibited specific proteasomal inhibitors MG-132 and lactacystin. Three commercial rabbit sera recognizing 20S core subunits...
We analyzed embryo-maternal interactions in the bovine uterus on day 8 of development. Proteomic profiles were obtained by two-dimensional difference gel electrophoresis from paired samples uterine fluid (UF) same animal with and without embryos uterus. Results contrasted UF after artificial insemination. detected 50 differential protein spots (t test, p < 0.05). Subsequent characterization nano-LC-ESI-MS/MS enabled us to identify 38 proteins, obtaining for first time earliest evidence...
Oviduct-specific glycoprotein (OGP) displays estrus-associated regional and temporal differences in expression localizes to the zona pellucida, perivitelline space, plasma membrane of oviductal oocytes embryos, suggesting that it may have a role regulation fertilization and/or early embryonic development. The aims this study were evaluate effect exogenous OGP on vitro (IVF) embryo development pig using defined serum-free culture system. In vitro-matured porcine incubated with homologous (0,...
Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, ploidy in vitro produced bovine embryos. Subsequently, using real-time PCR, the expression genes expected to underlie observed at blastocyst stage. matured oocytes were either fertilized or activated with ionomycin +6-DMAP cultured simple medium....
The bovine endometrium recognizes early embryos and reacts differently depending on the developmental potential of embryo. However, it is unknown whether can distinguish embryonic sex. Our objective was to analyze sexual dimorphism in uterus response male female embryos. Differentially expressed (DE) proteins, different levels hexoses, other embryotrophic differences were analyzed uterine fluid (UF). Proteomic analysis day-8 UF recovered from heifers after transfer day-5 or identified 23 DE...
The objectives of this research were to determine the effects β-mercaptoethanol (β-ME) and fetal bovine serum (FBS) on in vitro development embryos derived from oocytes matured fertilized examine mechanism through which β-ME may influence embryo development. A 2 × factorial treatment arrangement was used evaluate effect 0 or 100 µM 0% 10% FBS cultured Medium 199 (M199) absence somatic cells. Embryos randomly allocated within stage (< 8 cells 8–16 cells) one four combinations for 6 days. Both...
Pluripotency can be captured in vitro, providing that the culture environment meets requirements avoid differentiation while stimulating self-renewal. From studies mouse embryo, two kinds of pluripotent stem cells have been obtained from early and late epiblast, embryonic (ESCs) epiblast (EpiSCs), representing naive primed states, respectively. All attempts to derive convincing ESCs ungulates unsuccessful, although all were based on assumption conditions used or human ESC could applied other...
We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured synthetic oviduct fluid with BSA for 24 h before transfer. Spent CM, blank controls, samples (Day 0 7) evaluated FTIR. The spectra obtained analyzed. discrimination capability the classifiers was assessed...
Early in cow embryo development, hepatoma-derived growth factor (HDGF) is detectable uterine fluid. The origin of HDGF maternal tissues unknown, as the effect induction on developing embryos. Herein, we analyze expression day 8 endometrium exposed to embryos, well effects recombinant (rHDGF) growth. Exposure embryos did not alter endometrial levels mRNA or protein. protein localized cell nuclei luminal epithelium and superficial glands apical cytoplasm deep glands. After passage, embryonic...
Semen cryopreservation in bovine livestock is well established, but logistics often require deviations from standard protocols. Extending the equilibration time to following day convenient many situations. To improve our knowledge of effects this modification, we studied post-thawing and post-incubation (4 h, 38 °C) sperm quality after freezing with 4 or 24-h extension OPTIXcell extender by using an ample panel analyses: CASA for motility; flow cytometry viability, physiology, oxidative...
Major vault protein (MVP), also called lung resistance-related is a ribonucleoprotein comprising major part (>70%) of the particle. The function particle not known, although it appears to be involved in multi-drug resistance and cellular signaling. Here we show that MVP expressed mammalian, porcine, human ova porcine preimplantation embryo. was identified by matrix-assisted laser-desorption ionization-time-of-flight (MALDI-TOF) peptide sequencing Western blotting as accumulating zygotes...
In contrast to the embryos derived from live animals, produced in vitro undergo increased damage and reduced survival after cryopreservation, particularly when with serum. medium containing serum, retinoic acid increases cell numbers inner mass trophectoderm without altering their relative proportions bovine blastocyst. this work, we analyzed contribution of development blastocyst vitrification, found a strong reduction compared trophectoderm. Day-6 vitro-produced morulae were treated for 24...