A5001237277- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Biomedical Text Mining and Ontologies
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- Genetic Mapping and Diversity in Plants and Animals
- Birth, Development, and Health
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Endometriosis Research and Treatment
- Bioenergy crop production and management
- T-cell and B-cell Immunology
- Semantic Web and Ontologies
- Immune Cell Function and Interaction
- Genetic Syndromes and Imprinting
- Fermentation and Sensory Analysis
- T-cell and Retrovirus Studies
Oniris
2025
Fish Physiology and Genomics Institute
2022-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2016-2024
Université de Versailles Saint-Quentin-en-Yvelines
2024
Université Paris-Saclay
2016-2024
Biologie de la Reproduction, Environnement, Epigénétique et Développement
2021-2022
Laboratoire de Biologie du Développement
2010-2019
École Nationale Vétérinaire d'Alfort
2009-2019
Centre National de la Recherche Scientifique
1986-2019
Institut National de la Recherche Agronomique
1997-2014
Implantation is crucial for placental development that will subsequently impact fetal growth and pregnancy success with consequences on postnatal health. We postulated the pattern of genes expressed by endometrium when embryo becomes attached to mother uterus could account final outcome a pregnancy. As model, we used bovine species where progressively permanently from day 20 gestation onwards. At stage, compared endometrial profiles in presence an vivo fertilized (AI) patterns obtained...
At implantation the endometrium undergoes modifications necessary for its physical interactions with trophoblast as well development of conceptus. We aim to identify endometrial factors and pathways essential a successful in caruncular (C) intercaruncular (IC) areas cattle. Using 13,257-element bovine oligonucleotide array, we established expression profiles at day 20 estrous cycle or pregnancy (implantation), revealing 446 1,295 differentially expressed genes (DEG) C IC areas, respectively...
Syncytins are envelope genes of retroviral origin that have been co-opted for a role in placentation and likely contribute to the remarkable diversity placental structures. Independent capture events identified primates, rodents, lagomorphs, carnivores, where they involved formation syncytium layer at fetomaternal interface via trophoblast cell–cell fusion. We searched similar within suborder Ruminantia placenta lacks an extended but displays heterologous cell-fusion process unique among...
Transcription profiling of placentomes derived from somatic cell nuclear transfer (SCNT, n = 20), in vitro fertilization (IVF, 9), and artificial insemination (AI, 9) at or near term development was performed to better understand why SCNT IVF often result placental defects, hydrops, large offspring syndrome (LOS). Multivariate analysis variance used distinguish the effects SCNT, IVF, AI on gene expression, taking into account parturition (term preterm), sex fetus, breed dam, pathological...
Significance Cloning cattle by somatic cell nuclear transfer (SCNT) is an agriculturally important technology and also used as a model system for the study of mammalian development. The SCNT process inefficient, typically yielding fewer than 10% live offspring. majority losses are result embryonic death, failure implantation process, development defective placenta. A critical period window, when survival conceptus depends on factors including genetics, epigenetics, communication between...
The main objective of this project is to identify mRNA associated with oocyte maturation and embryonic developmental competency. knowledge genes their accumulated essential better understand the mechanisms involved in survival vitro produced embryo. We used bovine slaughterhouse-recovered ovaries collected oocytes from two follicle size categories: <2 mm 3–5 mm. content follicles where considered be more competent when compared In report we compare different technical approaches both...
Ig germline genes have been isolated from recombinant clones prepared in separate libraries constructed adult BALB/c liver DNA either pBR328 plasmid or EMBL 3 phage. Three that gave a very strong positive hybridization signal with VH anti-GAT-specific probe were completely characterized and sequenced. All three greater than 95% homologous, the exception of 5' noncoding region, which was only 85% homologous but contained characteristic regulatory signals. One these genes, H10, had sequence...
In the process of analyzing contribution nonproductive alpha- and beta-chain gene rearrangements to allelic exclusion TCR expression, we have found a novel type aberrant alpha-gene rearrangement. one alpha-allele mouse KB5-C20 T cell clone, J alpha segment has been abutted precisely sequence that does not display any homology known V D segment. The appended originates from within locus is located, in germ-line, 1 kb upstream member 2-gene subfamily. No recombination signal sequences...
Pluripotency can be captured in vitro, providing that the culture environment meets requirements avoid differentiation while stimulating self-renewal. From studies mouse embryo, two kinds of pluripotent stem cells have been obtained from early and late epiblast, embryonic (ESCs) epiblast (EpiSCs), representing naive primed states, respectively. All attempts to derive convincing ESCs ungulates unsuccessful, although all were based on assumption conditions used or human ESC could applied other...
We analyzed the change in gene expression related to dam physiological status day (D)18 embryos from growing heifers (GH), early lactating cows (ELC), and late (LLC). Dam energy metabolism was characterized by measurement of circulating concentrations insulin, glucose, IGF-1, nonesterified fatty acids, β-hydroxybutyrate, urea before embryo flush. The metabolic parameters were differential extraembryonic tissues correlation analysis. Embryo development estimated measuring length conceptuses...
Although high amounts of cyclin B1 mRNA are present in bovine oocytes arrested at the germinal vesicle (GV) stage, protein is not detectable. Furthermore, there a depletion stored oocyte as follicular growth progresses. To assess effect on accumulation M-phase promoting factor (MPF) components, and levels p34(cdc2) were measured GV collected from diverse follicle size groups (<2 mm, 3-5 >6 mm). Because very small follicles have mRNA, onset its was evaluated by situ hybridization fetal...
The high rates of embryonic mortalities which follow in vitro production ruminant embryos have emphasized the need for increased knowledge early development. It is likely that failures development and placenta formation involve abnormal differentiation mesoderm. aim this study was to investigate pattern expression two T-box genes known control gastrulation process, Brachyury Eomesodermin, by whole-mount situ hybridization. To allow a more precise comparison both patterns between embryos, we...