A5053993029- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Ovarian function and disorders
- Chromosomal and Genetic Variations
- Gene expression and cancer classification
- Reproductive Physiology in Livestock
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- Birth, Development, and Health
- Prenatal Screening and Diagnostics
- Blood Coagulation and Thrombosis Mechanisms
- Cancer-related gene regulation
- Reproductive System and Pregnancy
Université Laval
2015-2024
Physiologie Environnement et Génétique pour l'Animal et les Systèmes d'Elevage
2024
Institut Agro Rennes-Angers
2024
Quebec Network for Research on Aging
2021-2022
Ministère de l'Agriculture
2022
Inventiva (France)
2020
University of Milan
2015
Reproductive Science Center
2012
National Institute of Environmental Health Sciences
2012
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2012
Even after several decades of quiescent storage in the ovary, female germ cell is capable reinitiating transcription to build reserves that are essential support early embryonic development. In current model mammalian oogenesis, there exists bilateral communication between gamete and surrounding cells limited paracrine signaling direct transfer small molecules via gap junctions existing at end somatic cells' projections contact with oolemma. The purpose this work was explore role cumulus as...
The development of an accurate method for selection high-quality embryos is essential to achieve high pregnancy rates with single embryo transfer in human IVF. developmental competence the oocyte acquired during follicle maturation and strong communication also exists between follicular cells (FCs) oocytes; thus may be determined by markers expressed surrounding FCs. From consenting patients (n = 40), FCs were recovered on a per basis individual puncture. Hybridization analyses using...
In mammals, the study of gene expression in preimplantation embryo has been difficult because standard procedures used to quantify mRNA generally require large amounts starting material. The development protocols using different quantitative strategies involving polymerase chain reaction (PCR) provided new tools for exploration embryos. However, use an internal standard, often referred as a housekeeping gene, is essential normalize levels. RNA levels eight genes were quantified real time PCR...
So far, the characteristics of a good quality egg have been elusive, similar to nature physiological, cellular, and molecular cues leading its production both in vivo vitro. Current understanding highlights strong complex interdependence between follicular cells gamete. Secreted factors induce cellular responses cells, direct exchange small molecules from cumulus oocyte through gap junctions controls meiotic arrest. Studying interconnection oocyte, we previously demonstrated that somatic...
In advanced chronic liver disease (ACLD), deregulated hepatic necroinflammatory processes play a key role in the development of microvascular dysfunction, fibrogenesis, and increased vascular tone, resulting progression ACLD portal hypertension. Given current lack an effective treatment, we aimed to characterise effects pan-peroxisome proliferator-activated receptor (pan-PPAR) agonist lanifibranor 2 preclinical models ACLD, as well cells from patients with ACLD.Cirrhotic rats (thioacetamide...
Local translation at the synapse plays key roles in neuron development and activity-dependent synaptic plasticity. mRNAs are translocated from neuronal soma to distant synapses as compacted ribonucleoparticles referred RNA granules. These contain many RNA-binding proteins, including Fragile X Mental Retardation Protein (FMRP), absence of which results Syndrome, most common inherited form intellectual disability leading genetic cause autism. Using FMRP a tracer, we purified specific...
Spermiogenesis represents the transition from haploid spermatids to spermatozoa. This process entails an extreme condensation of nucleus and a loss nearly all cytoplasmic content. The presence messenger RNAs in spermatozoa has previously been shown. Generally, these transcripts are considered be remnants spermiogenesis. However, it recently proposed that there may exist function for sperm-associated RNAs. To address possibility functional role transcripts, we sought investigate characterize...
Abstract While most assisted reproductive technologies (ART) are considered routine for the reproduction of species economical importance, such as bovine, impact these manipulations on developing embryo remains largely unknown. In an effort to obtain a comprehensive survey bovine transcriptome and how it is modified by ART, resources were combined design embryo‐specific microarray. Close one million high‐quality reads produced from subtracted libraries using Roche 454 Titanium deep...
Early embryonic loss and altered gene expression in vitro produced blastocysts are believed to be partly caused by aberrant DNA methylation. However, specific stage which is sensitive culture conditions alter the methylation profile of resulting remained unclear. Therefore, aim this study was investigate effect environment on response blastocysts. For this, embryos cultured until zygote (ZY), 4-cell (4C) or 16-cell (16C) were transferred recipients recovery at day 7 estrous cycle. Another...
Aberrant DNA methylation patterns of genes required for development are common in vitro produced embryos. In this regard, we previously identified altered vivo developed blastocysts from embryos which spent different stages vitro, indicating carryover effects suboptimal culture conditions on epigenetic signatures preimplantation However, responses originated to not fully understood. Therefore, here investigated derived bovine subjected condition before, during or after major embryonic genome...
Abstract Extracellular vesicles (EVs) are nanoparticles secreted by ovarian follicle cells. an important form of intercellular communication, since they carry bioactive contents, such as microRNAs (miRNAs), mRNAs, and proteins. MicroRNAs small noncoding RNA capable modulating mRNA translation. Thus, EVs can play a role in oocyte development. However, it is not clear if EV contents vary with the estrous cycle stage. The aim this study was to investigate bovine miRNA content obtained from...
Abstract Current genetic methods of population assessment in conservation biology have been challenged by genome‐scale analyses due to their quantitatively novel insights. These include assessments runs‐of‐homozygosity (ROH), genomic evolutionary rate profiling (GERP), and mutational load. Here, we aim elucidate the relationships between these measures using three divergent ungulates: white‐tailed deer, caribou, mountain goat. The deer is currently expanding, while caribou are midst a...
Mammalian oocytes are very unique cells with an unlimited developmental potential. These totipotent able to remove existing gene-expression patterns and impose new ones. However, genome reprogramming is still a mystery. Posttranslational modifications by acetylation of the N-termini portion histones composing nucleosome involved in reprogramming. alter higher-order chromatin structure render DNA accessible regulatory transcriptional machinery. In present study, we have investigated, our...
Background and AimsCrop protection strategies, based on preventing quantitative crop losses rather than pest outbreaks, are being developed as a promising way to reduce fungicide use. The Bastiaans' model was applied winter wheat crops (Triticum aestivum) affected by leaf rust (Puccinia triticina) Septoria tritici blotch (STB; Mycosphaerella graminicola) under range of management conditions. This study examined (a) whether green area per layer accurately accounts for growth loss; (b) from...
Embryo selection efficiency in human IVF procedure is still suboptimal as shown by low pregnancy rates with single embryo transfer (SET). Bidirectional communication between the oocyte and follicular cells (FC) essential to achieve developmental competence of oocyte. Differences gene expression profile FCs from follicles leading could provide useful markers competence. were recovered individual follicle puncture. FC levels potential assessed Q-PCR an intra-patient inter-patient analysis...
Intercellular gap-junctional communication (GJC) plays an important role in ovarian cell physiology. Closure of GJC has been proposed to be involved oocyte maturation, particularly the resumption meiosis, both vivo and vitro, by controlling flow meiosis inhibitors, such as cAMP cGMP. Understanding how dynamics are regulated during vitro maturation (IVM) could provide a powerful tool for meiotic vitro. Since little is known about dynamic regulation between cumulus cells, we have developed...
Although the oocyte is largest cell in body and an unavoidable phase life, its physiology still poorly understood, other types provide little insight into unique nature. Even basic cellular functions such as energy metabolism are not yet fully understood. It known that mitochondria of female gamete exhibit immature form characterized by limited production from glucose oxidative phosphorylation. We show bovine uses alternative means to maintain ATP during maturation, namely, adenosine salvage...
Abstract Using a mouse model, studies by our group reveal that paternal preconception alcohol intake affects offspring fetal-placental growth, with long-lasting consequences on adult metabolism. Here, we tested the hypothesis chronic male exposure impacts histone enrichment in sperm and these changes are associated altered developmental programming placenta. chromatin immunoprecipitation, find alcohol-induced increases H3 lysine 4 trimethylation (H3K4me3) map to promoters presumptive...
Abstract Glioma pathogenesis‐related 1‐like protein1 (GliPr1L1) was identified by liquid chromatography‐tandem mass spectrometry analyses of proteins associated to bovine sperm lipid raft membrane domains. This protein belongs the CAP superfamily including cysteine‐rich secretory proteins, Antigen 5 and 1 protein. PCR analysis revealed that GliPr1L1 is expressed in testis and, at a much lower level, all along epididymis. Western blotting showed similar distribution testicular epididymal...