Cell-cell communication within the follicle involves many signaling molecules, and this process may be mediated by secretion uptake of exosomes that contain several bioactive molecules including extra-cellular miRNAs. Follicular fluid cells from individual follicles cattle were grouped based on Brilliant Cresyl Blue (BCB) staining corresponding oocytes. Both Exoquick precipitation differential ultracentrifugation used to separate exosome non-exosomal fraction follicular fluid. Following...

The purpose of this work is to address the relationship between transcriptional profile embryos and pregnancy success based on gene expression analysis blastocyst biopsies taken prior transfer recipients. Biopsies (30-40% intact embryo) were from in vitro-produced day 7 blastocysts (n = 118), 60-70% transferred recipients after reexpansion. Based pregnancy, pooled three groups (each 10 biopsies) namely: those resulting no (G1), resorbed (G2), calf delivery (G3). Gene these was performed...

The accumulation of maternal mRNA and protein during oogenesis for supporting oocyte maturation the newly fertilised zygote marks beginning developmental process in mammals. MicroRNAs (approximately 18-22 nt long) which are known post-transcriptional gene regulation evidenced their essential role animal development. We, therefore, aimed to investigate expression miRNAs immature vitro matured bovine oocytes, using heterologous miRNA array platform. To attain this, we used a mercury locked...

MicroRNAs are the major class of gene-regulating molecules playing diverse roles through sequence complementarity to target mRNAs at post-transcriptional level. Tightly regulated expression and interaction a multitude genes for ovarian folliculogenesis could be by these miRNAs. Identification them is first step towards understanding miRNA-guided gene regulation in different biological functions. Despite increasing efforts miRNAs identification across various species tissue types, little...

Summary We performed a genome‐wide QTL scan for production traits in line cross between Duroc and Pietrain breeds of pigs, which included 585 F 2 progeny produced from 31 full‐sib families genotyped with 106 informative microsatellites. A linkage map covering all 18 autosomes spanning 1987 Kosambi cM was constructed. Thirty‐five phenotypic including body weight, growth, carcass composition meat quality were analysed using least square regression interval mapping. Twenty‐four exceeded the...

Various environmental insults including diseases, heat and oxidative stress could lead to abnormal growth, functions apoptosis in granulosa cells during ovarian follicle growth oocyte maturation. Despite the fact that exposed are responding transcriptionally, potential release of transcripts associated with response into extracellular space through exosomes is not yet determined. Therefore, here we aimed investigate effect bovine vitro on cellular exosome mediated defense mechanisms. Bovine...

Summary MicroRNAs (miRNAs) are small endogenous molecules that involved in a diverse of cellular process. However, little is known about their abundance bovine oocytes and surrounding cumulus cells during oocyte development. To elucidate this situation, we investigated the relative expression pattern sets miRNAs between vitro maturation using miRNA polymerase chain reaction (PCR) array. Results revealed total 47 51 were highly abundant immature matured oocytes, respectively, compared with...

In bovine, ovarian follicles grow in a wave-like fashion with commonly 2 or 3 follicular waves emerging per estrous cycle. The dominant follicle of the wave which coincides LH-surge becomes ovulatory, leaving subordinate to undergo atresia. These physiological processes are controlled by timely and spatially expressed genes gene products, turn regulated post-transcriptional regulators. MicroRNAs, class short non-coding RNA molecules, one important posttranscriptional regulators associated...

SUMMARY In present study, we sought to examine the ability of preimplantation bovine embryos activate NF‐E2‐related factor 2 (NRF2)‐mediated oxidative‐stress response under an oxidative stress environment. vitro 2‐, 4‐, 8‐, 16‐cell‐, and blastocyst‐stage were cultured low (5%) or high (20%) oxygen levels. The expression NRF2 , KEAP1 (NRF2 inhibitor), antioxidants downstream NRF2, genes associated with embryo metabolism analyzed between groups using real‐time quantitative PCR. protein...

Aberrant gene expression in the uterine endometrium and embryo has been major causes of pregnancy failure cattle. However, selecting cows having adequate endometrial receptivity embryos better developmental competence based on pattern a greater challenge. To investigate whether pretransfer direct relation with upcoming success, we performed global transcriptome analysis using biopsy technology outcome information. For this, samples were collected from Simmental heifers at day 7 14 estrous...

The bi-directional communication between the oocyte and its companion cumulus cells (CCs) is crucial for development functions of both cell types. Transcripts that are exclusively expressed either in oocytes or CCs molecular mechanisms affected due to removal axis two types not investigated at a larger scale. main objectives this study were: 1. To identify transcripts 2. those which differentially when cultured with without vice versa.We analyzed transcriptome profile different CC samples...

This study aimed to investigate the miRNA expression patterns in granulosa cells of subordinate (SF) and dominant follicle (DF) during early luteal phase bovine estrous cycle. For this, enriched total RNA isolated from SF DF obtained heifers slaughtered at day 3 7 cycle was used for miRNAs deep sequencing. The results revealed that including 17 candidate novel miRNAs, several known (n = 291–318) were detected days which 244 common all groups. let-7 families, bta-miR-10b, bta-miR-26a,...

This study was conducted to investigate the gene expression profile of in vivo -derived bovine embryo biopsies based on pregnancy outcomes after transferring recipients. For this, 30–40% embryos were taken from grade I blastocysts (International Embryo Transfer Society Manual) and remaining 60–70% intact transferred Frozen pooled into three distinct groups outcome corresponding parts, namely those resulting no (NP), loss (PL), calf delivery (CD). Array analysis revealed a total 41 43 genes...

Clinical and subclinical endometritis are known to affect the fertility of dairy cows by inducing uterine inflammation. We hypothesized that clinical or could disturbing molecular milieu environment. Here we aimed investigate endometrial signatures pathways affected endometritis. For this, Holstein Frisian at 42-60 days postpartum were classified as healthy (HE), (SE) (CE) based on veterinary examination animals histological evaluation corresponding biopsies. Endometrial transcriptome...

Early embryonic loss and altered gene expression in vitro produced blastocysts are believed to be partly caused by aberrant DNA methylation. However, specific stage which is sensitive culture conditions alter the methylation profile of resulting remained unclear. Therefore, aim this study was investigate effect environment on response blastocysts. For this, embryos cultured until zygote (ZY), 4-cell (4C) or 16-cell (16C) were transferred recipients recovery at day 7 estrous cycle. Another...

Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in biological research due to the high-throughput and accuracy of qRT-PCR. For accurate reliable gene analysis, normalization data against housekeeping genes or internal control required. The stability reference has a tremendous effect on results relative quantification by could vary according tissues, age individuals experimental conditions. In pig however, very little information available genes. aim this...

Oocyte maturation and preimplantation embryo development are controlled by array of genes that post-transcriptionally regulated microRNAs. With respect to this, previously, we identified altered expression microRNA-130b (miR-130b) during oocyte maturation. Here, aimed investigate the role miR-130b in bovine granulosa cumulus cell function, using gain- loss-of- function approach. For this study, cells, cells oocytes were collected from ovaries obtained slaughterhouse. The targeted...

Aberrant DNA methylation patterns of genes required for development are common in vitro produced embryos. In this regard, we previously identified altered vivo developed blastocysts from embryos which spent different stages vitro, indicating carryover effects suboptimal culture conditions on epigenetic signatures preimplantation However, responses originated to not fully understood. Therefore, here investigated derived bovine subjected condition before, during or after major embryonic genome...

Nrf2 is a redox sensitive transcription factor regulating the expression of antioxidant genes as defense mechanism against various stressors. The aim this study to investigate potential role noncoding miRNAs endogenous and quercetin exogenous regulators pathway in bovine granulosa cells. For cultured cells were used for modulation (miR-28, 153 miR-708) targeting supplementation quercentin regulatory mechanisms system. Moreover, treated with hydrogen peroxide induce oxidative stress those Our...

Genomic selection has become a standard tool in dairy cattle breeding. However, for other animal species, implementation of this technology is hindered by the high cost genotyping. One way to reduce routine costs genotype candidates with an SNP (single nucleotide polymorphism) panel reduced density. This strategy investigated present paper. Methods are proposed approximation positions, SNPs be included low-density panel, imputation, and estimation accuracy genomic breeding values. The...

Despite its role in increasing the number of offspring during lifetime an individual animal, controlled ovarian hyperstimulation (COH) may have detrimental effects on oocyte development, embryo quality and endometrial receptivity. Circulating miRNAs bio-fluids been shown to be associated with various pathological conditions including cancers. Here we aimed investigate effect COH level extracellular bovine follicular fluid blood plasma elucidate their mode circulation potential molecular...

Large-scale expression profiling of micro-RNAs (miRNAs) in bovine granulosa cells from dominant and subordinate follicles on Day 19 the estrous cycle revealed enriched micro-RNA-183-96-182 cluster miRNAs preovulatory that coordinately regulate forkhead box protein O1 (FOXO1) gene. However, little is known about role this cell function. We used an vitro culture model to investigate role. Granulosa aspirated small growing (3-5 mm diameter) were cultured Dulbecco modified Eagle medium/F-12...

Satellite cells function as skeletal muscle stem to support postnatal growth and regeneration following injury or disease. There is great promise for the improvement of performance in livestock therapy pathologies humans by targeting myostatin (MSTN) this cell population. Human diet contains many histone deacetylase (HDAC) inhibitors, such bioactive component sulforaphane (SFN), whose epigenetic effects on MSTN gene satellite are unknown. Therefore, we aimed investigate influences SFN cells....