A5064988210- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- HIV-related health complications and treatments
- HIV, Drug Use, Sexual Risk
- Syphilis Diagnosis and Treatment
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- HIV/AIDS oral health manifestations
- Pneumocystis jirovecii pneumonia detection and treatment
- Cytomegalovirus and herpesvirus research
- Adolescent Sexual and Reproductive Health
- Infectious Diseases and Tuberculosis
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Reproductive tract infections research
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- HIV/AIDS Impact and Responses
- Long-Term Effects of COVID-19
- Research on Leishmaniasis Studies
- Viral-associated cancers and disorders
- Trypanosoma species research and implications
- Science and Education Research
Fundação Oswaldo Cruz
2016-2025
St James's University Hospital
2019-2020
University of Leeds
2019-2020
AIDS Clinical Trials Group
2016
National Institutes of Health
2016
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016
Ministério da Saúde
2016
Instituto Evandro Chagas
1999-2012
Johns Hopkins University
2011
Conference Board
2009
Present knowledge of human immunodeficiency virus type 1 (HIV-1) envelope immunobiology has been derived almost exclusively from analyses subtype B viruses, yet such viruses represent only a minority strains currently spreading worldwide. To generate more representative panel genetically diverse genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters the current AIDS...
Sequence analysis of a human immunodeficiency virus type 1 env gene PCR amplified from Brazilian woman's peripheral blood mononuclear cell DNA (sample RJIO1) showed that it was likely to have been derived double recombination event between subtypes B and F. The major portion the gp120 coding sequence belonged lineage, but segment C2 V3 region (approximately 135 nucleotides) clearly associated with sequences F lineage. subtype F-like had 15 noncontiguous signature nucleotides in common were...
To study the prevalence of HIV drug resistance mutations and subtype distribution in a Brazilian drug-naive population. Asymptomatic, HIV-1-infected individuals were targeted 13 voluntary counseling testing centers spread around country.Plasma viral RNA was extracted from 535 HIV-1-positive subjects. Protease (PR) reverse transcriptase (RT) genomic regions sequenced for determination analysis mutations.Eight samples (2.24 %) showed primary related to protease inhibitor (PI) resistance, eight...
Viral DNA sequences were determined over the V3 region of env from 28 infected individuals living in high HIV-1 prevalence Brazilian cities Rio de Janeiro and São Paulo. Twenty-six belonged to envelope sequence subtype B, prevalent North America Europe, one was classified as F, found recently Brazil Romania (one appeared be a B/F recombinant). Octameric at tip B loops variable distinct those Europe. The GPGR motif, American/European strains, only 8 (28.5%) sequences, whereas GWGR 12 (43%)...
To characterize the subtype C strains of HIV type 1 that circulate in Brazil, especially those originated from southern part country.One hundred and twelve HIV-1-positive subjects had their plasma viral RNA extracted. Protease (PR) reverse transcriptase (RT) genomic regions were polymerase chain reaction-amplified sequenced for determination. Subtype selected compared to other this database, specific amino acid signature patterns searched.Brazilian viruses form a very strong monophyletic...
In Brief Background Sexually transmitted infections (STIs) such as Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) can lead to adverse pregnancy neonatal outcomes. The prevalence of STIs its association with HIV mother-to-child transmission (MTCT) were evaluated in a substudy analysis from randomized, multicenter clinical trial. Methodology Urine samples HIV-infected pregnant women collected at the time labor delivery tested using polymerase chain reaction testing for detection CT...
Background. Evaluation of pretreatment HIV genotyping is needed globally to guide treatment programs. We examined the association (baseline) drug resistance and subtype with virologic failure in a multinational, randomized clinical trial that evaluated 3 antiretroviral (ART) regimens included resource-limited setting sites. Methods. Pol was performed nested case-cohort study including 270 randomly sampled participants (subcohort), 218 additional failing ART (case group). Failure defined as...
To investigate the origin and to reconstruct onset date of HIV-1 subtype C epidemic in Brazil.Three independent datasets sequences isolated from HIV-1-positive patients southern Brazil over a period 15 years (1991-2006) were analyzed: 82 env V3 (213 nt), 40 C2-C5 (559 72 pol (960 nt).Brazilian compared with other reference strains database using basic local alignment search tool, phylogenetic analyses, searching specific amino acid signature patterns. Evolutionary parameters estimated...
Untreated syphilis during pregnancy is associated with spontaneous abortion, stillbirth, prematurity and infant mortality. Syphilis may facilitate HIV transmission, which especially concerning in low- middle-income countries where both diseases are common.
Ongoing intra-host HIV-1 evolution has been shown in individuals that naturally suppress the viremia to low levels (HIV controllers) by analysis of RNA plasma compartment. Detection at DNA proviral compartment HIV controllers, however, more challenging and precise correlation between systemic viral suppression level rate reservoir’s reseeding those is not fully understood. In this sense, we examined quasispecies single genome amplification env gene a cohort 23 controllers from Brazil,...
Sexually transmitted infections (STIs) in pregnancy such as Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) may lead to adverse infant outcomes.Individual urine specimens from HIV-infected pregnant women diagnosed with HIV during labor were collected at the time of birth tested by polymerase chain reaction for CT NG. Infant infection was determined 3 months morbidity/mortality assessed through 6 months.Of 1373 maternal samples, 277 (20.2%) positive and/or NG; 249 (18.1%) CT, 63...
Background Sexually transmitted infections (STIs) including Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Treponema pallidum (TP), and cytomegalovirus (CMV) may lead to adverse pregnancy infant outcomes. The role of combined maternal STIs in HIV mother-to-child transmission (MTCT) was evaluated mother-infant pairs from NICHD HPTN 040. Methodology Urine samples HIV-infected pregnant women during labor were tested by polymerase chain reaction (PCR) for CT, NG, CMV. Infant infection...
Background Men who have sex with men (MSM) are more vulnerable to blood-borne infections and/or sexually-transmitted (STI). This study was conducted estimate the prevalences of mono and co-infections HIV-1 other blood-borne/STIs in a sample MSM Campinas, Brazil. Methods Responding Driven Sampling (RDS) used for recruitment MSM. Serum samples collected from 558 were analyzed presence serological markers HIV-1, HBV, HCV, HTLV, HPV-16/18, T. pallidum infections. Results The highest infection...
Background. Leishmania infection is a cofactor in the heightened cellular activation observed patients with American visceral leishmaniasis and human immunodeficiency virus type 1 (HIV) infection, or without progression to AIDS (AVL/HIV). Thus, persistence of high parasite load despite antileishmanial therapy could be responsible for continued immune stimulation.
To investigate the impact of early combined antiretroviral therapy (cART) on inflammation biomarkers and immune activation during acute chronic HIV-1 infection. We included 12 (AHI), 11 (EcHI), 18 late HIV-1-infected (LcHI) individuals who were treated with cART HIV-1-uninfected (HIV-neg) individuals. Plasmatic levels biomarkers, CD8+CD38+HLA-DR+ T cell frequencies, CD4 counts, CD4/CD8 ratio, total DNA plasmatic viral load evaluated. Mann–Whitney test, Pearson Spearman correlation, linear...
Background: Severe COVID-19 presents a variety of clinical manifestations associated with inflammatory profiles. People living HIV (PLWH) could face higher risk hospitalization and mortality from COVID-19, depending on their immunosuppression levels. This study describes markers in outcomes without infection. Methods: We analyzed 112 inpatients the Hospital Center for (INI/FIOCRUZ), including 22 cases PLWH (COVID/PLWH group). Plasma samples were tested panel 15 cytokines by Luminex....
Summary: The Brazilian Network for HIV Isolation and Characterization was established the surveillance of variability in Brazil. Here, we report characterization strains virus-specific immune responses from 35 clinical samples collected three potential vaccine sites. Three genetic subtypes HIV-1 were identified by heteroduplex mobility assay (HMA) B (in 82.9% samples), F (14.3%), C (2.9%). Phylogenetic analysis based on C2V3/env DNA sequence all 25 specimens examined 100% concordant with HMA...
HIV-1-positive individuals were recruited from January 1993 to December 1996 several cohorts receiving follow-up in the city of Rio de Janeiro, Brazil, evaluate HIV-1 genetic variability and potential association with modes transmission. subtyping was carried out using heteroduplex mobility assay (HMA), those samples corresponding typical Brazilian subtype B variant further identified based on Fok I restriction fragment length polymorphism (RFLP). DNA sequencing performed one case D...
We describe the genetic variability of HIV-1 subtypes and recombinant genomes in samples from southeastern southern Brazilian regions. Phylogenetic analysis a subset 34 (8F, 7B, 7C, 2D, 1A, 9 B″ variant) based on DNA sequencing env gp120 gp41, gag p17, nef regions confirmed presence nine (26.5%) potentially genomes. From eight C2–V3 subtype F samples, only two seem to be pure F. One classified as B gp41 had clustering with C. Two seven C presented distinct patterns Bgag/Cenv/Bnef...