A5053339332- Zebrafish Biomedical Research Applications
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Aquaculture disease management and microbiota
- T-cell and B-cell Immunology
- Acute Lymphoblastic Leukemia research
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Toxin Mechanisms and Immunotoxins
- Animal Genetics and Reproduction
- Invertebrate Immune Response Mechanisms
- interferon and immune responses
- Erythrocyte Function and Pathophysiology
- Developmental Biology and Gene Regulation
- Genomic variations and chromosomal abnormalities
- Acute Myeloid Leukemia Research
- Hemoglobinopathies and Related Disorders
- Bacillus and Francisella bacterial research
- Transgenic Plants and Applications
- Histone Deacetylase Inhibitors Research
- Antimicrobial Resistance in Staphylococcus
- Biomedical Ethics and Regulation
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Multiple Myeloma Research and Treatments
Century Therapeutics (United States)
2023-2024
Huntsman Cancer Institute
2008-2021
University of Utah
2009-2021
Bristol-Myers Squibb (United States)
2020-2021
Acetylon Pharmaceuticals (United States)
2015-2017
Columbia University Irving Medical Center
2017
Mount Sinai Hospital
2017
Mount Sinai Hospital
2017
Boston Children's Hospital
1992-2015
Harvard University
1992-2015
The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction clonally derived T cell acute lymphoblastic leukemia in transgenic expressing mouse c- myc under control Rag2 promoter. Visualization leukemic cells a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose thymus, spread locally into gill arches and retro-orbital soft tissue, then disseminated...
Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on previously published murine protocol. The solutions are sufficient quality to allow PCR detection transgenes all commonly used tissues. In sperm, transgene amplification was successful even when diluted 1000-fold, allowing easy identification transgenic founders. Given its speed and low cost, anticipate adoption this will streamline research.
Transgenic zebrafish that express GFP under control of the T cell-specific tyrosine kinase (lck) promoter were used to analyze critical aspects immune system, including patterns cell development and homing after transplant. GFP-labeled cells could be ablated in larvae by either irradiation or dexamethasone added water, illustrating have evolutionarily conserved responses chemical radiation ablation. In transplant experiments, thymocytes from lck-GFP fish repopulated thymus irradiated...
Spleen Knockout Explained Isolated congenital asplenia (ICA) is a rare disorder where patients are born without spleen and at increased risk of bacterial infection but have no other developmental abnormalities. Through sequence analysis familial sporadic cases, Bolze et al. (p. 976 , published online 11 April) found that ICA carry mutations in the gene encoding ribosomal protein SA as result express about half normal amount this protein. The mechanism by which reduced expression housekeeping...
Chimeric antigen receptor (CAR) T cells are a new and powerful class of cancer immunotherapeutics that have shown potential for the treatment hematopoietic malignancies. The tremendous promise this approach is tempered by safety concerns, including potentially fatal neurotoxicity, sometimes but not universally associated with cytokine release syndrome. We describe postmortem examination brain from 21-year-old patient relapsed pre-B cell acute lymphoblastic leukemia (ALL) who died fulminant...
Abstract Ligands that bind MHC class II (Ia) molecules, including staphylococcal exotoxins (SE) and mAb induce IL-1 TNF secretion in human monocytes monocytic cell lines. In this study, we have analyzed the mechanisms by which SE beta TNF-alpha production. Treatment of peripheral blood with exotoxin B toxic shock syndrome toxin-1 resulted a biphasic increase mRNA lasted more than 12 h transient rise mRNA. A F(ab) preparation anti-HLA DR L243 also caused significant monokine levels....
We have studied the transcriptional activation of human TNF-alpha gene by superantigen staphylococcal enterotoxin A (SEA) in premonocytic cell line THP-1. Nuclear proteins from SEA-stimulated THP-1 cells bound strongly to kappa 3, most proximal three putative NF-kappa B binding sites (kappa 1-kappa 3) found 5' regulatory region gene, but only weakly 1, distal sites, and showed no 2. The mobility 3-nucleoprotein complex was identical that complexes formed between nuclear consensus seuqence....
Hematopoiesis is precisely orchestrated by lineage-specific DNA-binding proteins that regulate transcription in concert with coactivators and corepressors. Mutations the zebrafish moonshine (mon) gene specifically disrupt both embryonic adult hematopoiesis, resulting severe red blood cell aplasia. We report mon encodes ortholog of mammalian transcriptional intermediary factor 1gamma (TIF1gamma) (or TRIM33), a member TIF1 family During development, hematopoietic progenitor cells mutants fail...
For the last three decades significant parts of national science budgets, and international private funding worldwide, have been dedicated to cancer research. This has resulted in a number important scientific findings. Studies tissue culture multiplied our knowledge cell pathophysiology, mechanisms transformation strategies survival cells, revealing therapeutically exploitable differences normal cells. Rodent animal models provided insights on developmental biology cells host responses...
The novel immune-type receptor (NITR) genes encode a unique multigene family of leukocyte regulatory receptors, which possess an extracellular Ig variable (V) domain and may function in innate immunity. Artificial chromosomes that zebrafish NITRs have been assembled into contig spanning ≈350 kb. Resolution the complete NITR gene cluster has led to identification eight previously undescribed families revealed presence C-type lectins within locus. A maximum haplotype 36 (138 sequences total)...
The ontogeny of haematopoietic niches in vertebrates is essentially unknown. Here we show that the stromal cells caudal tissue (CHT), first niche where definitive stem/progenitor (HSPCs) home zebrafish development, derive from somites through an epithelial–mesenchymal transition (EMT). resulting cell progenitors accompany formation vein sinusoids, other main component CHT niche, and mature into reticular lining interconnecting sinusoids. We characterize a mutant defective haematopoiesis due...