A5086057803- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- Enzyme-mediated dye degradation
- Cancer Cells and Metastasis
- Plant biochemistry and biosynthesis
- Liver Disease Diagnosis and Treatment
- Synthesis and Reactions of Organic Compounds
- Photosynthetic Processes and Mechanisms
- Biochemical effects in animals
- Endoplasmic Reticulum Stress and Disease
- PARP inhibition in cancer therapy
- Ubiquitin and proteasome pathways
- Immune cells in cancer
- Synthesis and Reactivity of Heterocycles
- Microbial metabolism and enzyme function
- Cancer therapeutics and mechanisms
- Hepatitis C virus research
- Liver physiology and pathology
- Bioactive Natural Diterpenoids Research
- Phytochemical compounds biological activities
- Synthesis and pharmacology of benzodiazepine derivatives
- Cancer, Stress, Anesthesia, and Immune Response
- Chemical Synthesis and Analysis
- Electrostatic Discharge in Electronics
National Research Council
2015-2025
Institute for Experimental Endocrinology and Oncology
2023-2025
National Research Council
2023
Institute of Protein Biochemistry
2015-2022
Ceinge Biotecnologie Avanzate (Italy)
2006-2020
University of Naples Federico II
2012-2014
National Agency for New Technologies, Energy and Sustainable Economic Development
2013
Federico II University Hospital
2006-2012
University of Cagliari
2008-2011
Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies
2010
Abstract The response to antiviral therapy is lower in hepatitis C virus (HCV) patients with genotype 1 than those 2. Overexpression of the suppressor cytokine signaling 3 (SOCS3) gene liver tissue associated a poorer treatment outcome chronic viral 1. Also, insulin resistance has been implicated nonresponse an anti-HCV treatment. To understand why HCV respond differently, we investigated SOCS3 expression, metabolic syndrome (MS), and cohort HCV-related hepatitis. A total 198 (108 90 2)...
Abstract Poly-ADP-ribose-polymerases (PARPs) 1 and 2 are nuclear enzymes that catalyze the poly-ADP-ribosylation of proteins transferring poly-ADP-ribose (PAR) polymers to specific residues. PARPs PAR intervene in diverse functions, including DNA repair nucleus stress granule assembly cytoplasm. Stress granules contribute regulation translation by clustering stabilizing mRNAs as well several cytosolic signaling modulate cell metabolism survival. Our study is focused on one these PARPs,...
Ubiquitination is a reversible post-translational modification based on the chemical addition of ubiquitin to proteins with regulatory effects various signaling pathways. can alter molecular functions tagged substrates respect protein turnover, biological activity, subcellular localization or protein–protein interaction. As result, wide variety cellular processes are under ubiquitination-mediated control, contributing maintenance homeostasis. It follows that dysregulation ubiquitination...
Hepatocellular carcinoma is one of the most common cancers worldwide. Despite several efforts to elucidate hepatocellular molecular pathogenesis, it still not fully understood. To acquire further insights into mechanisms carcinoma, we performed a systematic functional genomic approach on human HuH-7 and JHH-6 cells. The subsequent analysis differentially expressed genes in specimens revealed signature 11 from which selected LGALS1 gene, was overexpressed carcinoma. expression humans...
ABSTRACT In mammalian cells, the Golgi complex is composed of stacks that are connected by membranous tubules. During G2, disassembled into isolated stacks. This process required for entry mitosis, indicating correct inheritance organelle monitored a ‘Golgi mitotic checkpoint’. However, regulation and molecular mechanisms underlying this disassembly still poorly understood. Here, we show JNK2 has crucial role in G2-specific separation through phosphorylation Ser277 Golgi-stacking protein...
Huntington's disease (HD) is a neuropsychiatric disorder characterized by choreiform movement of the limbs, cognitive disability, psychosis and dementia. It invariably associated with an abnormally long CAG expansion within IT15 gene on human chromosome 4. Although mutant huntingtin protein ubiquitously expressed in HD patients, cellular degeneration occurs predominantly neurons corpus striatum cerebral cortex. The Ras homolog Rhes very selectively precise brain areas affected HD. Recent...
Abstract The Golgi apparatus is composed of stacks cisternae laterally connected by tubules to form a ribbon-like structure. At the onset mitosis, ribbon broken down into discrete stacks, which then undergo further fragmentation. This cleavage required for G2/M transition, thus indicates that ‘Golgi mitotic checkpoint’ couples inheritance with cell cycle transition. We previously showed Golgi-checkpoint regulates centrosomal recruitment kinase Aurora-A; however, how unlinking this was...
The development and the function of central nervous system depend on thyroid hormones. In humans, lack hormones causes cretinism, a syndrome severe mental deficiency. It is assumed that affect normal brain by activating or suppressing target gene expression because several genes expressed in have been shown to be under hormone control. Among these, Rhes gene, encoding small GTP-binding protein, predominantly striatal region brain. To clarify role vivo, we disrupted homologous recombination...
Several genes encoding for proteins involved in proliferation, invasion, and apoptosis are known to be direct miR-34a targets. Here, we used proteomics screen targets of neuroblastoma (NBL), a childhood cancer that originates from precursor cells the sympathetic nervous system. We examined effect overexpression using tetracycline inducible system two NBL cell lines (SHEP SH-SY5Y) at early time points expression (6, 12, 24 h). Proteome analysis post-metabolic labeling led identification 2,082...
Immunotherapy approaches targeting the PD-1 pathway have shown some clinical benefits in a fraction of patients with lung cancer, but expression PD-L1 has proven to be an imperfect biomarker efficacy.Recent studies that tumor mutation burden (TMB) is also correlated outcome and it appears independent PD-L1.TMB, however, only indirectly measures number neoantigenic peptides presented on cell surface class I MHC predicted matches may even better predictor benefit.In addition, mutations genes...
Significance Proteins are modified by many posttranslational modifications (PTMs) with crucial regulatory functions. A PTM attracting increasing interest is ADP-ribosylation, capable of altering cellular targets. We show that mono-ADP-ribosylation PARP12 the protein Golgin-97 regulates transport to plasma membrane a specific group functionally cargo proteins. shown be part cascade initiated PKD and involving direct phosphorylation activation PARP12. These events define, through...