A5065416200- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Mechanisms and Therapy
- Immune cells in cancer
- Immune Response and Inflammation
- Virus-based gene therapy research
- Vector-borne infectious diseases
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Diabetes and associated disorders
- Medicinal Plant Pharmacodynamics Research
- Cancer Cells and Metastasis
- Pharmacogenetics and Drug Metabolism
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- Dermatological diseases and infestations
- Neuropeptides and Animal Physiology
- Cancer Research and Treatments
- Pancreatic function and diabetes
- Viral Infections and Vectors
- Complement system in diseases
- Glycosylation and Glycoproteins Research
New York University
2007-2018
NYU Langone Health
1998-2018
CS Diagnostics
2016
York University
2016
Institute of Cell Biology
2010-2012
Laboratory of Molecular Genetics
2012
Kaplan (United States)
2006
Medical University of South Carolina
2002
University of Münster
1996-2000
Columbia University Irving Medical Center
1983-1999
Foxp3 activity is essential for the normal function of immune system. Two types regulatory T (T reg) cells express Foxp3, thymus-generated natural reg (nT cells, and peripherally generated adaptive (iT cells. These cell have complementary functions. Until now, it has not been possible to distinguish iT from nT in vivo based solely on surface markers. We report here that Neuropilin 1 (Nrp1) expressed at high levels by most cells; contrast, mucosa-generated other noninflammatory low Nrp1....
The distribution of newly synthesized proteolipid protein (PLP, 23 kdaltons) and myelin basic proteins (MBPs, 14-21.5 was determined in microsomal fractions prepared from the brainstems o1 10-30 d-old rats sacrificed at different times after an intracranial injection 35S-methionine. Labeled MBPs were found fraction 2 min injection, whereas PLP appeared first rough only a lag 30 fraction. Cell-free translation experiments using purified mRNAs demonstrated that are bound free polysomes,...
The transition of chronic pancreatic fibroinflammatory disease to neoplasia is a primary example the paradigm linking inflammation carcinogenesis. However, cellular and molecular mediators bridging these entities are not well understood. Because TLR4 ligation can exacerbate inflammation, we postulated that activation drives In this study, show lipopolysaccharide accelerates tumorigenesis, whereas inhibition protective. Furthermore, blockade MyD88-independent TRIF pathway protective against...
Abstract IFNs protect from virus infection by inducing an antiviral state and modulating the immune response. Using mice deficient in multiple aspects of IFN signaling, we found that type I II play distinct although complementing roles resolution influenza viral disease. Both types influenced profile cytokines produced T lymphocytes, with a significant bias toward Th2 differentiation occurring absence responsiveness to either IFN. However, Th1 through inhibition IFN-γ was not required...
Hepatic fibrosis occurs during most chronic liver diseases and is driven by inflammatory responses to injured tissue. Because DCs are central modulating immunity, we postulated that altered DC function contributes immunologic changes in hepatic affects the pathologic milieu within fibrotic liver. Using mouse models, determined contribution of immunity investigated role environment We found depletion completely abrogated elevated levels many mediators produced represented approximately 25%...
A rabbit antiserum was prepared against the C-terminal peptide of 21 amino acids from human heat shock protein hsp70. These antibodies were shown to be specific for this highly inducible (72 kilodaltons [kDa] in rat cells), and a moderately inducible, constitutively expressed protein, hsc70 (74 kDa). In six independently derived cell lines transformed by murine cDNA-genomic hybrid clone p53 plus an activated Ha-ras gene, elevated levels detected immunoprecipitation using murine-specific...
Abstract NK cell functions were examined in mice with a targeted mutation of the STAT1 gene, an essential mediator IFN signaling. Mice deficient displayed impaired basal cytolytic activity vitro and unable to reject transplanted tumors vivo, despite presence normal numbers cells. IL-12 enhanced NK-mediated cytolysis, but poly(I:C) did not, similar phenotype occurred lacking IFNα receptors. Molecules involved activation lytic function cells (granzyme A, granzyme B, perforin, DAP10, DAP12)...
Fifteen peptides, ranging in length from 6 to 31 amino acids and corresponding sequence portions of the major phenobarbital-inducible form rat liver cytochrome P-450 (P-450 PB-4), were previously synthesized chemically used prepare site-specific rabbit antibodies (Frey, A. B., D.J. Waxman, G. Kreibich, 1985, J. Biol. Chem., 260:15253-15265). The antipeptide affinity purified using Sepharose resins derivatized with respective peptides 14 preparations obtained that an ELISA assay showed...
Abstract We have investigated the ability of different cells present in murine tumors to induce apoptosis activated CD8+ T vitro. Tumor do not cells; however, macrophages that infiltrate are potent inducers apoptosis. express cell surface-associated TNF, TNF type I (CD120a) and II (CD120b) receptors, and, upon contact with which induces release IFN-γ from cells, secrete nitric oxide. Killing vitro is blocked by Abs IFN-γ, CD120a, or CD120b, N-methyl-l-arginine. In concert finding, tumor...
Tumor-infiltrating lymphocytes (TIL) are well known to be functionally impaired typified by the inability lyse cognate tumor cells in vitro. We have investigated basis for defective TIL lytic function transplantable murine models. CD8(+) nonlytic immediately on isolation even though they express surface activation markers, contain effector phase cytokine mRNAs, and perforin granzyme B proteins which packaged into granules. Ag-specific capability is rapidly recovered if purified briefly...
The site of synthesis and mechanism insertion into membranes several microsomal polypeptides was studied using translation system programmed in vitro with polysomes or mRNA extracted from free membrane‐bound rat liver polysomes. Primary products cytochrome b 5 , NADH: oxidoreductase, NADPH: P‐450 oxidoreductase epoxide hydrolase were isolated by specific immunoprecipitation compared the mature proteins. following observations made: While are synthesized polysomes, made In all cases...
The normal liver is characterized by immunologic tolerance. Primary mediators of hepatic immune tolerance are sinusoidal endothelial cells (LSECs). LSECs block adaptive immunogenic responses to Ag and induce the generation T regulatory cells. Hepatic fibrosis both intense intrahepatic inflammation altered immunity. We postulated that, in fibrosis, a reversal LSEC function from tolerogenic proinflammatory may contribute heightened inflammatory milieu found after fibrotic injury hepatotoxins,...
Abstract The expansion of distinct immune suppressive cells in the liver tumor bearing hosts may bear on propensity patients with intra-abdominal cancers to develop metastases. is most common site adenocarcinoma metastases, even who initially present early disease. We postulated that immune-suppressive tumor-bearing inhibit anti-tumor T cells, thereby accelerating growth Using models preinvasive pancreatic neoplasia and advanced colorectal cancer, aims this study were determine phenotype,...
Oligomeric protein complexes containing the nuclear oncogene p53 and simian virus 40 large tumor antigen (D. I. H. Linzer A. J. Levine, Cell 17:43-51, 1979), adenovirus E1B 55-kilodalton (kDa) antigen, heat shock hsc70 (P. Hinds, C. Finlay, Frey, Mol. Cell. Biol. 7:2863-2869, 1987) have all been previously described. To begin isolating, purifying, testing these for functional activities, we developed a rapid immunoaffinity column purification. p53-protein are eluted from by using molar...
8 mM Na2HP04, 2.7 KCI, 1.5 KHzPO.,, pH 7.4); PhCHSOZF,