A5064958093- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- Nanofabrication and Lithography Techniques
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Pharmacological Effects of Medicinal Plants
- Natural Compounds in Disease Treatment
- Immune cells in cancer
- Diabetes and associated disorders
- Chemotherapy-induced organ toxicity mitigation
- Bone Metabolism and Diseases
- Andrographolide Research and Applications
- Neuroblastoma Research and Treatments
- Protein purification and stability
Chongqing Medical and Pharmaceutical College
2019-2025
University of Southern California
2023-2024
Georgia Institute of Technology
2017-2024
The Wallace H. Coulter Department of Biomedical Engineering
2022-2024
Parker Hannifin (United States)
2022-2024
Woodruff Health Sciences Center
2022
Woodruff Scientific (United States)
2019
Yuxian People's Hospital
2015
University of Hong Kong
2013
Abstract Despite the clinical success of blocking its interactions, how PD-1 inhibits T-cell activation is incompletely understood, as exemplified by potency far exceeding what might be predicted from affinity for ligand-1 (PD-L1). This may partially attributed to PD-1’s targeting proximal signaling receptor (TCR) and co-stimulatory CD28 via activating Src homology region 2 domain-containing phosphatases (SHPs). Here, we report regulates initial TCR antigen recognition manifested in a...
Low-density lipoprotein (LDL) is intricately associated with numerous clinical conditions, including dyslipidemia and metabolic-associated fatty liver disease (MAFLD), its serum concentration crucial for diagnostic purposes. However, the sensitive accurate analysis of "intact" LDL a significant difficulty, as conventional approaches typically focus solely on detection cholesterol or surface proteins LDL. We developed proximity ligation-induced DNAzyme motor that facilitates an outstanding...
Conventional approaches for studying receptor-mediated cell signaling, such as the western blot and flow cytometry, are limited in three aspects: 1) The perturbing preparation procedures often alter molecules from their native state on cell; 2) Long processing time before final readout makes it difficult to capture transient signaling events (<1 min); 3) experimental environments force-free, therefore unable visualize mechanical signals real time. In contrast these methods biochemistry...
Immunotherapy approaches targeting the PD-1 pathway have shown some clinical benefits in a fraction of patients with lung cancer, but expression PD-L1 has proven to be an imperfect biomarker efficacy.Recent studies that tumor mutation burden (TMB) is also correlated outcome and it appears independent PD-L1.TMB, however, only indirectly measures number neoantigenic peptides presented on cell surface class I MHC predicted matches may even better predictor benefit.In addition, mutations genes...
In this report, the mechanism of antitumor activities Kushen flavonoids (KS-Fs) were explored. KS-Fs and kurarinone (Kur), a single flavonoid compound, able to induce apoptosis H460 Eca-109 cells in vitro vivo. The inducing effect was enhanced presence Taxol. xenograft mice treated with Kur, down-regulation Bcl-2 up-regulation caspase 8 3 tumors observed by immunohistochemical staining. addition, Kur inhibit TNFalpha-induced NF-kappaB activation 293 mediated decreased IkappaBalpha...
ABSTRACT Selectins and integrins are key players in the adhesion signaling cascade that recruits leukocytes to inflamed tissues. Selectin binding induces β2 integrin slow leukocyte rolling. Here, a micropipette was used characterize neutrophil E-selectin intercellular molecule-1 (ICAM-1) at room temperature. The time-dependent frequency displayed two-stage kinetics, with an E-selectin-mediated fast increase low plateau followed by high mediated intermediate-affinity of αLβ2 ICAM-1....
Abstract For the clinical diagnosis of diseases and for basic biological research, it is crucial to develop a trustworthy efficient method detecting small extracellular vesicles (sEVs) in multiple experimental conditions. Here, we create colorimetric assay that enables sensitive precise sEVs identification without need pricey equipment. In this assay, exonuclease III (Exo III)-assisted signal recycle activated by released single-strand DNA (ssDNA) from SMBs (streptavidin magnetic...
The interaction of the human FcγRIIA with immune complexes (ICs) promotes neutrophil activation and thus must be tightly controlled to avoid damage healthy tissue. Here, we demonstrate that a fungal-derived soluble β-1,3/1,6-glucan binds glycosphingolipid long-chain lactosylceramide (LacCer) reduce FcγRIIA-mediated recruitment immobilized ICs under flow, process requiring high-affinity FcγRIIA-immunoglobulin G (IgG) interactions. inhibition requires Lyn phosphorylation SHP-1 phosphatase...
fashion as evidenced by luciferase reporter assays.In summary, this study demonstrates that AGP inhibits osteoclastogenesis in RAW 264.7 murine macrophage cells through downregulation of the NF-
Force-mediated molecular binding initiates numerous cellular activities such as cell adhesion, migration, and activation. Dynamic force spectroscopy (DFS) is widely used to examine mechano-signaling... | Find, read cite all the research you need on Tech Science Press
Immune checkpoint blockade targeting PD-1 shows great success in cancer therapy. However, the mechanism of how ligand binding initiates signaling remains unclear. As prognosis markers multiple cancers, soluble PD-L1 is found patient sera and can bind PD-1, but fails to suppress T cell function. This our previous observations that cells exert endogenous forces on PD-1-PD-L2 bonds prompt hypothesis mechanical force might be critical triggering, which missing case due lack support afforded by...
As an emerging class of noninvasive biomarkers, accurate quantification circulating extracellular vesicles (EVs) is essential to both the basic biological research and early diagnosis pancreatitis. We report here allosteric probe-initiated cascade amplification system for a highly sensitive detection serum-circulating EVs. In this method, special recognition EVs by probes triggers allosterism probe thus induces release signal initiator. Through following multiple rounds amplifications, large...
Abstract Adaptation is a cellular process in which cells are progressed from the starting sensitive state to later adapted exposing agent. Acute exposure of cadmium (Cd) can cause cell death; however, chronic Cd develop acquired tolerance where death suppressed. We previously reported that during adaptation lung epithelial cells, death, generally due perturbation c-Jun N-terminal kinase (JNK) signaling pathway and nonresponsiveness JNK phosphorylation critical for Cd-tolerance these (1, 2)....
Abstract Although much clinical progress has been made in harnessing the immune system to recognize and target cancer, there is still a significant lack of an understanding how tumors evade recognition mechanisms that drive tumor resistance both T-cell checkpoint blockade immunotherapy. Our objective understand tumor-mediated signaling through inhibitory receptors, including PD-1, combines affect process antigen activation signaling. This goal basis PD-1 potentially identifying new molecular...