A5089909580- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Genetic factors in colorectal cancer
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Retinal Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Ferroptosis and cancer prognosis
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Glaucoma and retinal disorders
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
Guangzhou Experimental Station
2022-2025
Inner Mongolia People's Hospital
2025
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2019-2024
Guangzhou Institutes of Biomedicine and Health
2020-2024
Chinese Academy of Sciences
2024
Max Planck Institute for Molecular Biomedicine
2014-2023
Guangzhou Medical University
2022-2023
Third Affiliated Hospital of Guangzhou Medical University
2023
Zhuhai People's Hospital
2021
StemCells (United States)
2018
Phenotypic drug discovery requires billions of cells for high-throughput screening (HTS) campaigns. Because up to several million different small molecules will be tested in a single HTS campaign, even variability within the cell populations could easily invalidate an entire campaign. Neurodegenerative assays are particularly challenging because neurons post-mitotic and cannot expanded implementation HTS. Therefore, neuroprotective compounds type that is robustly expandable able...
purpose. In the context of retinal ganglion cell (RGC) axon degeneration in optic nerve that occurs glaucoma, microglia become activated, then phagocytic, and redistribute head. The authors investigated potential contribution activation to glaucoma progression DBA/2J chronic mouse model. methods. treated 6-week-old mice for 25 weeks with minocycline, a tetracycline derivative known reduce improve neuronal survival other models neurodegenerative disease. They quantified RGC numbers...
Abstract The process of induced pluripotent stem cells (iPSCs) reprogramming involves several crucial events, including the mesenchymal-epithelial transition (MET), activation genes, metabolic reprogramming, and epigenetic rewiring. Although these events intricately interact influence each other, specific element that regulates network remains unclear. Dux, a factor known to promote totipotency during from embryonic (ESC) 2C-like ESC (2CLC), has not been extensively studied in context iPSC...
Article6 March 2015Open Access Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile Fumihiro Sugawa Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany Search more papers by this author Marcos J Araúzo-Bravo Group Computational Biology Bioinformatics, Biodonostia Health Research Institute, San Sebastián, Spain IKERBASQUE, Basque Foundation Science, Bilbao, Juyong Yoon Kee-Pyo Kim Shinya...
Oct4 is widely considered the most important among four Yamanaka reprogramming factors. Here, we show that combination of Sox2, Klf4, and cMyc (SKM) suffices for mouse somatic cells to induced pluripotent stem (iPSCs). Simultaneous induction Sox2 in fibroblasts triggers immediate retroviral silencing, which explains discrepancy with previous studies attempted but failed generate iPSCs without using vectors. SKM could partially activate pluripotency network, even Oct4-knockout fibroblasts....
Genetically modified domestic animals have many potential applications ranging from basic research to production agriculture. One of the goals in transgenic animal schemes is reliably predict expression pattern foreign gene. Establishing a method screen genetically embryos for transgene before transfer surrogates may improve likelihood producing offspring with desired pattern. In order determine how be regulated early embryo, we generated porcine two distinct cell lines by using nuclear (NT)...
The separation of the first two lineages – trophectoderm (TE) and inner cell mass (ICM) is a crucial event in development early embryo. ICM, which constitutes pluripotent founder population, develops into embryo proper, whereas TE, comprises surrounding outer layer, supports ICM before after implantation. Cdx2, transcription factor expressed specifically developing for differentiation cells as lack zygotic Cdx2 expression leads to failure embryos hatch implant uterus. However, speculation...
Naive and primed pluripotent stem cells (PSCs) germ express the Oct4 gene. The gene contains two cis-regulatory elements, distal enhancer (DE) proximal (PE), which differentially control expression in a cell-type-specific stage-specific manner. Here, we generated double transgenic mice carrying both Oct4-ΔPE-GFP Oct4-ΔDE-tdTomato (RFP), enabling us to simultaneously monitor activity of DE PE. is stage-specifically regulated by PE during embryonic cell development. Using this dual reporter...