A5036670286- Pluripotent Stem Cells Research
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Mitochondrial Function and Pathology
- Reproductive Biology and Fertility
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Bacteriophages and microbial interactions
- Erythrocyte Function and Pathophysiology
- Renal and related cancers
- Molecular Biology Techniques and Applications
- Mesenchymal stem cell research
- Advanced Electron Microscopy Techniques and Applications
- Heat shock proteins research
- Physiological and biochemical adaptations
- Advanced Fluorescence Microscopy Techniques
- Retinal Imaging and Analysis
- CRISPR and Genetic Engineering
- Prion Diseases and Protein Misfolding
- Invertebrate Immune Response Mechanisms
- Antibiotic Resistance in Bacteria
- Proteins in Food Systems
- RNA Research and Splicing
Osnabrück University
2017-2025
Max Planck Institute for Molecular Biomedicine
2011-2023
Max Planck Society
2015
Article6 March 2015Open Access Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile Fumihiro Sugawa Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany Search more papers by this author Marcos J Araúzo-Bravo Group Computational Biology Bioinformatics, Biodonostia Health Research Institute, San Sebastián, Spain IKERBASQUE, Basque Foundation Science, Bilbao, Juyong Yoon Kee-Pyo Kim Shinya...
In vitro culture systems that structurally model human myogenesis and promote PAX7 + myogenic progenitor maturation have not been established. Here we report skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm neuromesodermal progenitors develop into organized structures reassembling neural plate border dermomyotome. Culture conditions instigate lineage arrest fetal hypaxial toward limb axial anatomical identity, with...
Endosomes are central organelles in the recycling and degradation of receptors membrane proteins. Once endocytosed, such proteins sorted at endosomes into intraluminal vesicles (ILVs). The resulting multivesicular bodies (MVBs) then fuse with lysosomes, leading to ILVs monomers. However, biogenesis MVBs requires a constant lipid supply for efficient ILV formation. An ER-endosome contact site has been suggested play critical role MVB biogenesis. Here, we identify Any1 as novel phospholipid...
Epigenetic memory in induced pluripotent stem cells, which is related to the somatic cell type of origin might lead variations differentiation capacities cells. In this context, cells from human CD34(+) hematopoietic be more suitable for than commonly used fibroblast-derived To investigate influence an epigenetic on ex vivo expansion into erythroid we compared neural and cord blood-derived evaluated their potential progenitor mature red blood Although genome-wide DNA methylation profiling at...
Abstract Synthetically replicating key biological processes requires the ability to puncture lipid bilayer membranes and remodel their shape. Recently developed artificial DNA nanopores are one possible synthetic route due ease of fabrication. However, an unresolved fundamental question is how bind dynamically interact with bilayers. Here we use single-molecule fluorescence microscopy establish that carrying cholesterol anchors insert via a two-step mechanism into membranes. Nanopores...
Several studies have pointed to retinal involvement in COVID-19, yet many questions remain regarding the ability of SARS-CoV-2 infect and replicate cells its effects on retina. Here, we used human pluripotent stem cell-derived organoids study infection by SARS-CoV-2. Indeed, can organoids, as it is shown different lineages, such ganglion photoreceptors. also induces expression several inflammatory genes, interleukin 33, a gene associated with acute COVID-19 degeneration. Finally, show that...
Abstract Early mouse embryos have an atypical translational machinery that consists of cytoplasmic lattices and is poorly competent for translation. Hence, the impact transcriptomic changes on operational level proteins predicted to be relatively modest. To investigate this, we performed liquid chromatography–tandem mass spectrometry mRNA sequencing at seven developmental stages, from mature oocyte blastocyst, independently validated our data by immunofluorescence qPCR. We detected...
F1FO ATP synthase, also known as complex V, is a key enzyme of mitochondrial energy metabolism that can synthesize and hydrolyze ATP. It not whether the synthase ATPase function are correlated with different spatio-temporal organisation enzyme. In order to analyze this, we tracked localized single molecules in situ using live cell microscopy. Under normal conditions, V was mainly restricted cristae indicated by orthogonal trajectories along membranes. addition confined quasi immobile exist....
Aging is closely associated with cardiovascular diseases, the leading cause of mortality worldwide. Mitochondrial dysfunction a hallmark aging because it generates most heart's ATP at cristae, specialized sub-compartments where OXPHOS takes place. In this study, we used multiple-scale electron microscopy approaches to evaluate age-related mitochondrial and cristae ultrastructural alterations in human mouse hearts. We found that aged patients' hearts displayed reduced density as seen by TEM,...
Abstract Ischemic heart disease and by extension myocardial infarction is the primary cause of death worldwide, warranting regenerative therapies to restore function. Current models natural regeneration are restricted in that they not adult mammalian origin, precluding study class-specific traits have emerged throughout evolution, reducing translatability research findings humans. Here, we present spiny mouse ( Acomys spp .), a murid rodent exhibits bona fide back skin ear pinna, as model...
The plasma membrane of a cell is subject to stresses causing ruptures that must be repaired immediately preserve integrity and ensure survival. Yet, the spatio-temporal dynamics at wound site source required for repair are poorly understood. Here, it shown early endosomes, previously only known function in uptake extracellular material its endocytic transport, involved human endothelial cells. Using live-cell imaging correlative light electron microscopy, demonstrated injury triggers unknown...
The orchestrated activity of the mitochondrial respiratory or electron transport chain (ETC) and ATP synthase convert reduction power (NADH, FADH2) into ATP, cell's energy currency in a process named oxidative phosphorylation (OXPHOS). Three out four ETC complexes are found supramolecular assemblies: complex I, III, IV form supercomplexes (SC). plasticity model suggests that SC formation is adaptation to changing conditions such as supply, redox state, stress. Complex NADH-dehydrogenase,...
Abstract The facultative intracellular pathogen Salmonella enterica remodels the host endosomal system for survival and proliferation inside cells. resides within Salmonella- containing vacuole (SCV) by -induced fusions of endomembranes, SCV is connected with extensive tubular structures termed induced filaments (SIF). lifestyle critically depends on effector proteins translocated into A subset effectors associated with, or integral in SIF membranes. How reach their subcellular destination,...
Mitochondrial F1FO ATP synthase is the key enzyme for mitochondrial bioenergetics. Dimeric F1FO-ATP synthase, preferentially located at edges of cristae and its oligomerization state determines ultrastructure. The inhibitor protein IF1 modulates not only activity but also regulates both structure function mitochondria. In order to understand this in more detail, we have investigated effect on spatiotemporal organization synthase. Stable cell lines were generated that overexpressed...
Endosome biogenesis in eukaryotic cells is critical for nutrient uptake and plasma membrane integrity. Early endosomes initially contain Rab5, which replaced by Rab7 on late prior to their fusion with lysosomes. Recruitment of requires the Mon1-Ccz1 guanine-nucleotide-exchange factor (GEF). Here, we show that full function Drosophila complex a third stoichiometric subunit, termed Bulli (encoded CG8270). localises Rab7-positive endosomes, agreement its GEF complex. Using nephrocytes as model...
Correlative light and electron microscopy (CLEM) combines (LM) of fluorescent samples to ultrastructural analyses by (EM). Pre-embedding CLEM often suffers from inaccurate correlation between LM EM modalities. Post-embedding enables precise registration structures directly on sections, but requires markers withstanding sample preparation, especially osmium tetroxide fixation, dehydration EPON embedding. Most proteins (FPs) lose their fluorescence during such conventional embedding (CE),...
Germ cells are a unique population of responsible for transmitting genetic information from one generation to the next. Our understanding key mechanisms underlying germ cell development in vivo remains scarce because insufficient amounts materials available conducting biological studies. The establishment vitro differentiation models that support mouse pluripotent stem provides an alternative means studying reproductive development. detection and analysis cell-derived cells, however, present...
Abstract In vitro culture systems that structurally model human myogenesis and promote PAX7 + myogenic progenitor maturation have not been established. Here we report skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm neuromesodermal progenitors develop into organized structures reassembling neural plate border dermomyotome. Culture conditions instigate lineage arrest fetal hypaxial towards limb axial anatomical identity,...
Dysfunction of the aging heart is a major cause death in human population. Amongst other tasks, mitochondria are pivotal to supply working with ATP. The mitochondrial inner membrane (IMM) ultrastructure tailored meet these demands and provide nano-compartments for specific tasks. Thus, function morphology closely coupled. Senescent cardiomyocytes from mouse display alterations membrane. To study relation between architecture, dynamics hardly possible living organisms. Here, we present two...
ABSTRACT Gasdermin D (GSDMD) executes inflammatory cell death pyroptosis by permeabilizing the plasma membrane (PM). We introduce polymer-supported PM (PSPM) to gain access cytoplasmic side of with imaging techniques while preserving native complexity and lipid microenvironment. By combining PSPM DNA-PAINT super-resolution microscopy we visualized, for first time, GSDMD nanostructures directly at pyroptotic cells. resolved diverse macromolecular architectures ring-and arc-shaped oligomers...
This work aimed at the development of a stable albumin-perfluorocarbon (o/w) emulsion as an artificial oxygen carrier suitable for clinical application. So far, albumin-perfluorocarbon-(o/w) emulsions have been successfully applied in preclinical trials. Cross-linking variety different physical and chemical methods characterization (PFC)-(o/w) was necessary to gain deep understanding its specific emulsification processes during high-pressure homogenization. High-pressure homogenization is...
Drosophila harbors a simple tubular heart that ensures hemolymph circulation within the body. The is built by few different cell types, including cardiomyocytes define luminal channel and ostia cells constitute openings in wall allowing to enter chamber. Regulation of flow directionality tube, such as blood arteries or insect lumen, requires dedicated gate, valve, flap-like structure prevents backflow fluids. In heart, intracardiac valves provide this streaming, with one valve being present...
While many proteins are known clients of heat shock protein 90 (Hsp90), it is unclear whether the transcription factor, thyroid hormone receptor beta (TRb), interacts with Hsp90 to control hormonal perception and signaling. Higher expression in mouse fibroblasts was elicited by addition triiodothyronine (T3). T3 bound enhanced adenosine triphosphate (ATP) binding due a specific site for T3, as identified molecular docking experiments. The TRb prevented or mimetic sobetirome. Purified...