A5075758578- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Cellular transport and secretion
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Receptor Mechanisms and Signaling
- Spectroscopy and Quantum Chemical Studies
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Mass Spectrometry Techniques and Applications
- Force Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Molecular Junctions and Nanostructures
- Nanopore and Nanochannel Transport Studies
- ATP Synthase and ATPases Research
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Sphingolipid Metabolism and Signaling
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Gold and Silver Nanoparticles Synthesis and Applications
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Pharmacological Effects and Assays
University of Fribourg
2017-2025
Institut de Pharmacologie Moléculaire et Cellulaire
2013-2024
Centre National de la Recherche Scientifique
2013-2024
Université Côte d'Azur
2015-2024
Inspire
2024
Inserm
2023-2024
Fribourg Development Agency
2021
Centre de recherche cerveau et cognition
2018
Centre National pour la Recherche Scientifique et Technique (CNRST)
2015
École Polytechnique Fédérale de Lausanne
2009-2014
In eukaryotic cells, phosphatidylserine (PS) is synthesized in the endoplasmic reticulum (ER) but highly enriched plasma membrane (PM), where it contributes negative charge and to specific recruitment of signaling proteins. This distribution relies on transport mechanisms whose nature remains elusive. Here, we found that PS transporter Osh6p extracted phosphatidylinositol 4-phosphate (PI4P) exchanged for PI4P between two membranes. We solved crystal structure Osh6p:PI4P complex demonstrated...
Bending the benefits of polyunsaturates We have often heard that it is beneficial to eat polyunsaturated fatty acids. also know some organelles such as synaptic vesicles are extremely rich in lipids. However, what lipids do our body unclear. Using cell biology, biochemical reconstitutions, and molecular dynamics, Pinot et al. show phospholipids can change response membranes proteins involved membrane curvature sensing, shaping, fission. Polyunsaturated make plasma more amenable deformation;...
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in biological sample and gives robust way quantify proteins at single-cell level from PALM images. apply determine amount β2 adrenergic receptor, prototypical G Protein Coupled Receptor, expressed on plasma membrane HeLa cells.
Lipids are unevenly distributed within eukaryotic cells, thus defining organelle identity. How non-vesicular transport mechanisms generate these lipid gradients between membranes remains a central question. Here using quantitative, real-time assays, we demonstrate that Osh4p, sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger of the ORP/Osh family, transports sterol against its gradient two by dissipating energy PI(4)P gradient. Sterol is sustained through maintenance this...
Multidomain proteins with flexible linkers and disordered regions play important roles in many cellular processes, but characterizing their conformational ensembles is difficult. We have previously shown that the coarse-grained model, Martini 3, produces too compact solution, may part be remedied by strengthening protein–water interactions. Here, we show decreasing strength of protein–protein interactions leads to improved agreement experimental data on a wide set systems. 'symmetry' between...
Significance Metabolic disorders related to aberrant fat accumulation, including lipodystrophy and obesity, are a serious health concern. Inside cells, accumulates in organelles named lipid droplets (LDs). LDs form the endoplasmic reticulum (ER), where triglycerides (TG), most abundant of fat, produced. The Berardinelli–Seip congenital type 2 protein, seipin, has been identified as key regulator LD formation, but its mechanism action remains debated molecular details mostly obscure. Here, we...
Cells store energy in the form of neutral lipids (NLs) packaged into micrometer-sized organelles named lipid droplets (LDs). These structures emerge from endoplasmic reticulum (ER) at sites marked by protein seipin, but mechanisms regulating their biogenesis remain poorly understood. Using a combination molecular simulations, yeast genetics, and fluorescence microscopy, we show that interactions between lipids’ acyl-chains modulate propensity NLs to be stored LDs, turn preventing or...
Abstract Cell growth is regulated by the mammalian/mechanistic target of rapamycin complex 1 (mTORC1), which functions both as a nutrient sensor and master controller virtually all biosynthetic pathways. This ensures that cells are metabolically active only when conditions optimal for growth. Notably, although mTORC1 known to regulate fatty acid biosynthesis, how whether cellular lipid capacity signals back fine-tune activity remains poorly understood. Here we show senses cell synthesise...
Glycerophospholipids are synthesized primarily in the cytosolic leaflet of endoplasmic reticulum (ER) membrane and must be equilibrated between bilayer leaflets to allow ER membranes derived from it grow. Lipid equilibration is facilitated by integral proteins called “scramblases.” These feature a hydrophilic groove allowing polar heads lipids traverse hydrophobic interior, similar credit card moving through reader. Nevertheless, despite their fundamental role expansion dynamics, identity...
Endosomes are central organelles in the recycling and degradation of receptors membrane proteins. Once endocytosed, such proteins sorted at endosomes into intraluminal vesicles (ILVs). The resulting multivesicular bodies (MVBs) then fuse with lysosomes, leading to ILVs monomers. However, biogenesis MVBs requires a constant lipid supply for efficient ILV formation. An ER-endosome contact site has been suggested play critical role MVB biogenesis. Here, we identify Any1 as novel phospholipid...
When small phosphatidylcholine liposomes are added to perforated cells, they bind preferentially the Golgi suggesting an exceptional avidity of this organelle for curved membranes without stereospecific interactions. We show that cis golgin GMAP-210 accounts property. First, liposome tethering properties resembles amphipathic lipid-packing sensor (ALPS) motif GMAP-210: both preferred (radius < 40 nm) made monounsaturated but not saturated lipids. Second, reducing levels or redirecting its...