A5036459333- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Congenital heart defects research
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- Cardiac Fibrosis and Remodeling
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant-Microbe Interactions and Immunity
- Marine Ecology and Invasive Species
- Acute Lymphoblastic Leukemia research
- Planarian Biology and Electrostimulation
- Plant and Fungal Interactions Research
- Chronic Myeloid Leukemia Treatments
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Cancer Genomics and Diagnostics
- Plant and Biological Electrophysiology Studies
Utrecht University
2023
University Medical Center Utrecht
2023
Royal Netherlands Academy of Arts and Sciences
2021-2022
Hubrecht Institute for Developmental Biology and Stem Cell Research
2010-2022
Institute of Molecular Biology
2014-2020
The University of Sydney
2017
Germ cells of most animals critically depend on piRNAs and Piwi proteins. Surprisingly, in mouse oocytes are relatively rare dispensable. We present compelling evidence for strong piRNA expression other mammals. Human fetal express PIWIL2 transposon-enriched piRNAs. Oocytes adult human ovary PIWIL1 PIWIL2, whereas those bovine only PIWIL1. In human, macaque, ovaries, we find that resemble testis-borne pachytene Isolated follicular were shown to contain abundant, short preferentially target...
Highlights•Tdrd6a is required for Bucky ball mobility within aggregates, and PGC formation•Maternal Tdrd6a coordinates transcript deposition into future PGCs•A dimethylated tri-RG motif in mediates interaction with Tdrd6a•The essential Balbiani body germ cell formationSummaryPhase separation represents an important form of subcellular compartmentalization. However, relatively little known about how the formation or disassembly such compartments regulated. In zebrafish, (Bb) plasm (Gp) are...
The role of the Piwi/piRNA pathway during mammalian oogenesis has remained enigmatic thus far, especially since experiments with Piwi knockout mice did not reveal any phenotypic defects in female individuals. This is striking contrast results obtained from other species including flies and zebrafish. In mouse oocytes, however, only low levels piRNAs are found they required for their function. We recently demonstrated dynamic expression PIWIL1, PIWIL2, PIWIL3 early embryogenesis. addition,...
Abstract Ischemic heart disease and by extension myocardial infarction is the primary cause of death worldwide, warranting regenerative therapies to restore function. Current models natural regeneration are restricted in that they not adult mammalian origin, precluding study class-specific traits have emerged throughout evolution, reducing translatability research findings humans. Here, we present spiny mouse ( Acomys spp .), a murid rodent exhibits bona fide back skin ear pinna, as model...
Viral infection triggers a range of plant responses such as the activation RNA interference (RNAi) pathway. The double-stranded binding (DRB) proteins DRB3 and DRB4 are part this pathway aid in defending against DNA viruses, respectively. Using live cell imaging, we show that DRB2, DRB3, DRB5 relocate from their uniform cytoplasmic distribution to concentrated accumulation nascent viral replication complexes (VRC) develop following invasion by RNA. Inactivation gene Arabidopsis T-DNA...
P-element induced wimpy testis (PIWIs) are crucial guardians of genome integrity, particularly in germ cells. While mammalian PIWIs have been primarily studied mouse and rat, a homologue for the human PIWIL3 gene is absent Muridae family, hence unique function cells cannot be effectively modeled by knockouts. Herein, we investigated expression, distribution, interaction bovine oocytes. We localized to mitochondria, demonstrated that expression stringently controlled both spatially temporally...
Summary In recent years, it has become clear that phase separation represents an important class of subcellular compartmentalization. However, relatively little is known about how the formation or disassembly such compartments regulated. zebrafish, Balbiani body (Bb) and germ plasm (Gp) are phase-separated structures essential for cell specification home to many cell-specific mRNAs proteins. Throughout development, these range from a single large aggregate (Bb), dispersed state back...
Abstract Ischemic heart disease and by extension myocardial infarction is the primary cause of death worldwide, necessitating regenerative therapies to restore function. Current models regeneration are restricted in that they not adult mammalian origin, precluding study class-specific traits have emerged throughout evolution, reducing translatability research findings humans. Here, we overcome those restrictions introducing African spiny mouse ( Acomys spp .), a murid rodent has recently...
Abstract PIWIs are crucial guardians of genome integrity, particularly in germ cells. While mammalian have been primarily studied mouse and rat, a homologue for the human PIWIL3 gene is absent Muridae family, hence unique function cells cannot be effectively modeled by knockouts. Herein, we investigated expression, distribution interaction bovine oocytes. We localized to mitochondria, demonstrated that expression stringently controlled both spatially temporally before after fertilization....