A5039555517- Hearing, Cochlea, Tinnitus, Genetics
- Vestibular and auditory disorders
- Nerve injury and regeneration
- Hearing Loss and Rehabilitation
- Neuroscience and Neural Engineering
- Heat shock proteins research
- Ear Surgery and Otitis Media
- Extracellular vesicles in disease
- Biochemical effects in animals
- Physiological and biochemical adaptations
- Photoreceptor and optogenetics research
- Connexins and lens biology
- Infrared Thermography in Medicine
- Advanced Sensor and Energy Harvesting Materials
- Advanced Biosensing Techniques and Applications
- Cancer Mechanisms and Therapy
- Noise Effects and Management
- MicroRNA in disease regulation
- Pain Management and Treatment
- Ion Channels and Receptors
- bioluminescence and chemiluminescence research
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Biochemical and Structural Characterization
- Inflammasome and immune disorders
Medizinische Hochschule Hannover
2020-2024
Hearing4all
2021-2024
Deutsche Forschungsgemeinschaft
2021-2024
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2021-2022
The variability in outcomes of cochlear implantation is largely unexplained, and clinical factors are not sufficient for predicting performance. Genetic have been suggested to impact outcomes, but the genetic heterogeneity hereditary hearing loss makes it difficult determine interpret postoperative It hypothesized that mutations affect neuronal components cochlea auditory pathway, targeted by implant (CI), may lead poor A large cohort CI recipients was studied verify this hypothesis.This...
The lack of approved anti-inflammatory and neuroprotective therapies in otology has been acknowledged the last decades recent approaches are heralding a new era field. Extracellular vesicles (EVs) derived from human multipotent (mesenchymal) stromal cells (MSC) can be enriched vesicular secretome fractions, which have shown to exert effects (eg, neuroprotection immunomodulation) their parental cells. Hence, MSC-derived EVs may serve as novel drug candidates for several inner ear diseases....
Cisplatin is a highly effective chemotherapeutic agent, but it can cause sensorineural hearing loss (SNHL) in patients. Cisplatin-induced ototoxicity closely related to the accumulation of reactive oxygen species (ROS) and subsequent death hair cells (HCs) spiral ganglion neurons (SGNs). Despite various strategies combat ototoxicity, only one therapeutic agent has thus far been clinically approved. Therefore, we have developed gene therapy concept protect cochlear from cisplatin-induced...
Despite a vast amount of data generated by proteomic analysis on cochlear fluid, novel clinically applicable biomarkers inner ear diseases have not been identified hitherto. The aim the present study was to analyze proteome human perilymph from implant patients, thereby identifying putative changes composition fluid due specific diseases. Sampling performed during implantation patients with or radiologically defined like enlarged vestibular aqueduct (EVA; n = 14), otosclerosis (n 10), and...
The high complexity of the cellular architecture human inner ear and inaccessibility for tissue biopsy hampers molecular analysis disease. Sampling perilymph may present an opportunity improved diagnostics understanding pathology. Analysis proteome from patients undergoing cochlear implantation was carried out revealing a multitude proteins patterns protein composition that enable characterisation into subgroups. Based on existing data databases, single are not in blood circulation were...
Mesenchymal stromal cells (MSCs) are an adult derived stem cell-like population that has been shown to mediate repair in a wide range of degenerative disorders. The protective effects MSCs mainly mediated by the release growth factors and cytokines thereby modulating diseased environment immune system. Within inner ear, have against tissue damage induced sound variety ototoxins. To better understand mechanism action mice were exposed narrow band noise. After exposure, from human umbilical...
State-of-the-art treatment for sensorineural hearing loss is based on electrical stimulation of residual spiral ganglion neurons (SGNs) with cochlear implants (CIs). Due to the anatomical gap between electrode contacts CI and afferent fibers SGNs, spatial spreading signal hampers focused neuronal stimulation. Also, efficiency a limited because SGNs degenerate over time due trophic support. A promising option close install as artificial nerve guidance structures surface implant these drug...
While many proteins are known clients of heat shock protein 90 (Hsp90), it is unclear whether the transcription factor, thyroid hormone receptor beta (TRb), interacts with Hsp90 to control hormonal perception and signaling. Higher expression in mouse fibroblasts was elicited by addition triiodothyronine (T3). T3 bound enhanced adenosine triphosphate (ATP) binding due a specific site for T3, as identified molecular docking experiments. The TRb prevented or mimetic sobetirome. Purified...
Background: Survival and integrity of the spiral ganglion is vital for hearing in background noise optimal functioning cochlear implants. Numerous studies have demonstrated that supplementation supraphysiologic levels neurotrophins BDNF NT-3 by pumps or gene therapy strategies supports survival. The endogenous physiological growth factors within inner ear, although difficult to determine, are likely extremely low normal ear. Thus, novel approaches long-term low-level delivery may be...
Introduction Panendoscopy is an important diagnostic and therapeutic tool in head neck surgery. The associated biopsies can cause significant postoperative pain. Aim of this study was to determine a possible correlation between pain the inflammatory factors IL-1β, IL-6 TNFα without with intake acetylcysteine, mucolytic anti-inflammatory effects.
Einleitung Die Panendokopie ist ein wichtiges diagnostisches und therapeutisches Instrument in der Kopf-Hals-Chirurgie. damit verbundenen Biopsien können erhebliche postoperative Schmerzen verursachen. Ziel dieser Studie war es, einen möglichen Zusammenhang zwischen postoperativen Entzündungsfaktoren IL-1β, IL-6 TNFα ohne mit Einnahme von Acetylcystein, einem Mucolytikum antiinflammatorischer Wirkung, zu ermitteln.
Abstract Cisplatin is a chemotherapeutic agent widely used to treat solid tumors. However, it can also be highly ototoxic, resulting in high-frequency hearing loss. causes degeneration of hair cells (HCs) and spiral ganglion neurons (SGNs) the inner ear, which are essential components process cannot regenerated mammals. As affected primarily die by apoptosis, we tested several anti-apoptotic small molecules protect these from drug-induced toxicity. We found that general caspase inhibitor...
Objective The implantation of biohybrid electrodes was introduced a few years ago in our clinic. These coated with autologous mononuclear cells releasing anti-inflammatory and neuroprotective factors are thought to reduce insertion trauma maintain the vitality surviving spiral ganglion neurons. clinical feasibility this approach has already been demonstrated. In present retrospective study, four-year results two sides (classical electrode electrode) bilaterally implanted patients were...
ABSTRACT The use of optogenetic tools offers an excellent method for spatially and temporally regulated gene protein expression in cell therapeutic approaches. This could be useful as a concomitant measure, especially small body compartments such the inner ear, example, during cochlea implantation, to enhance neuronal survival function. Here, we used blue light activatable CRY2/CIB system induce transcription brain‐derived neurotrophic factor (BDNF) human cells. Transfection with three...
Mutations in connexin 26 (Cx26) cause hearing disorders of a varying degree. Herein, to identify compounds capable restoring the function mutated Cx26, novel miniaturized microarray-based screening system was developed perform an optical assay Cx26 functionality. These molecules were identified through viability using HeLa cells expressing wild-type (WT) which exhibited sensitivity toward HSP90 inhibitor radicicol submicromolar concentration range. Open hemichannels are assumed mediate...
Preservation of the excitability spiral ganglion neurons (SGN) may contribute to an improved speech perception after cochlear implantation. Thus, application exogenous neurotrophic factors such as neurotrophin brain-derived factor (BDNF) increase SGN survival in vitro and vivo is a promising pharmacological approach implant (CI) research. Due difficult pharmacokinetic profile proteins BDNF, there quest for small molecules mediate or efficacy BDNF. The C3 exoenzyme from Clostridium botulinum...
Einleitung Trotz rasanter Fortschritte sind die zugrundeliegenden zellulären und molekularen Mechanismen des Hörverlusts noch immer kaum verstanden. Die Entnahme molekulare Analyse der Perilymphe kann nicht nur das Verständnis pathologischen Prozesse im Innenohr verbessern, sondern auch neue Behandlungsstrategien ermöglichen.
Introduction Nowadays, corticosteroids are widely used to treat Meniere's disease or sudden deafness, and also during cochlear implantation. Corticosteroids have anti-inflammatory immunomodulatory effects. In this study, we four different already clinically tested (fludrocortisone, triamcinolone, dexamethasone, prednisolone) examined their effects on the survival rate neurite length of spiral ganglion neurons (SGN).
Einleitung Heutzutage werden Kortikosteroide häufig zur Behandlung von Morbus Menière oder plötzlicher Taubheit eingesetzt sowie unter anderem auch während der Cochlea-Implantation. besitzen entzündungshemmende und immunmodulatorische Wirkungen. In dieser Studie haben wir vier verschiedene bereits klinisch eingesetzte (Fludrocortison, Triamcinolon, Dexamethason Prednisolon) verwendet deren Wirkung auf die Überlebensrate Neuritenlänge Spiralganglienneuronen (SGN) untersucht.
Introduction Despite rapid progress, the underlying cellular and molecular mechanisms of hearing loss are still poorly understood. Sampling analysis perilymph may not only improve understanding pathological processes within inner ear but also enable novel treatment strategies.
Introduction Mesenchymal stromal cells (MSC) can be derived from various tissue types and are promising for the treatment of degenerative diseases. Modulation diseased extracellular milieu as well immune system seem to mediated via a paracrine effect (protective factors cytokines released by MSC). The aim present study was decipher possible molecular mechanisms action MSC.
Einleitung Die Verabreichung von einem Faktor-Cocktail zum Schutz und zur Regeneration Spiralganglienneuronen (SGN) erscheint in der Cochlea Implantat Forschung momentan am vielversprechendsten. Das plättchenreiche Plasma (PRP) enthält zahlreiche zellbasierte Wachstumsfaktoren fördert die Wundheilung. neuroprotektive Wirkung PRP auf SGN konnten wir bereits einer vorherigen Studie zeigen. Plättchenreiches Fibrin (PRF) ist noch natürlicher sehr reich an Leukozyten, gerinnt wenige Minuten nach...
Abstract This study uses an all‐optical approach to probe interneuronal communication between spiral ganglion neurons (SGNs) and of other functional units, in this case cortex (CNs) hippocampus (HNs), for the first time. We combined a channelrhodopsin variant (CheRiff) with red genetically encoded calcium indicator (jRCaMP1a), enabling simultaneous optical stimulation recording from spatially separated small neuronal populations. Stimulation SGNs was possible both optogenetic manipulated HNs...