Abstract Mesenchymal stem/stromal cells (MSCs) are a heterogenous population of multipotent and highly secretory currently being investigated in the field wound healing for their ability to augment tissue responses. The adaptive response MSC populations rigid substrate current 2D culture systems has been considered result deterioration regenerative ‘stem‐like’ properties. In this study, we characterise how improved adipose‐derived mesenchymal stem (ASCs) within tissue‐mimetic 3D hydrogel...

Background: The microfracture technique for cartilage repair has limited ability to regenerate hyaline cartilage. Aim: current study made a direct comparison between and an osteochondral approach with microsphere-based gradient plugs. Materials & methods: PLGA-based scaffolds had opposing gradients of chondroitin sulfate β-tricalcium phosphate. A 1-year in sheep was conducted. Results: tissues the were mostly fibrous scattered fissures degenerative changes. Cartilage regenerated plugs equal...

Abstract Background The secretome of adipose-derived mesenchymal stem cells (ASCs) offers a unique approach to understanding and treating wounds, including the critical process epidermal regeneration orchestrated by keratinocytes. However, 2D culture techniques drastically alter secretory dynamics ASCs, which has led ambiguity in secreted compounds (e.g., growth factors, exosomes, reactive oxygen species) may be driving epithelialization. Methods A novel tissue-mimetic 3D hydrogel system was...

Use of decellularized tissues has become popular in tissue engineering applications as the natural extracellular matrix can provide necessary physical cues that help induce restoration and development functional tissues. In relation to cochlear engineering, question whether act a scaffold support incorporation exogenous cells not been addressed. Investigators have explored composition developed multiple strategies for decellularizing variety different tissues; however, no one investigated...

The transcription factor atonal homolog 1 (ATOH1) has multiple homologues that are functionally conserved across species and is responsible for the generation of sensory hair cells. To evaluate potential functional differences between homologues, human mouse ATOH1 (HATH1 MATH-1, respectively) were nonvirally delivered to Wharton's jelly cells (hWJCs) first time. Delivery HATH1 hWJCs demonstrated superior expression inner ear cell markers characteristics than delivery MATH-1. Inhibition HES1...

To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to

Differentiating stem cells using gene delivery is a key strategy in tissue engineering and regenerative medicine applications. Nonviral bypasses several safety concerns associated with viral delivery; however, leading nonviral techniques, such as electroporation, subject to high stress can result poor cell viabilities. Inhibition of Rho-associated coiled-coil kinase (ROCK) has been shown mitigate apoptotic mechanisms detachment freezing induced pluripotent embryonic cells; inhibiting ROCK...

Introduction Wound healing consists of a dynamic series events that are highly dependent on paracrine factors for proper progression through the phases wound healing. Inappropriate is associated with insufficient epidermal regeneration (i.e., re-epithelialization) wounds and subsequent propagation chronic wounds, such as diabetic ulcers, which increased patient morbidity. Recently, investigation into secretome Adipose-derived Mesenchymal Stem Cells (ASCs), have shown promise in augmenting...

Abstract Hydrogels can be fabricated and designed to exert direct control over stem cells' adhesion differentiation. In this study, we have investigated the use of polydopamine (pDA)‐treatment as a binding platform for bioactive compounds create versatile gelatin–alginate (Gel–Alg) hydrogel tissue engineering applications. Precisely, pDA was used modify surface properties better osteogenic differentiation human adipose‐derived cells (hASCs). enabled adsorption different types molecules,...

Mesenchymal stromal cells (MSCs) are an adult derived stem cell-like population that has been shown to mediate repair in a wide range of degenerative disorders. The protective effects MSCs mainly mediated by the release growth factors and cytokines thereby modulating diseased environment immune system. Within inner ear, have against tissue damage induced sound variety ototoxins. To better understand mechanism action mice were exposed narrow band noise. After exposure, from human umbilical...

In mammals, mechanosensory hair cells that facilitate hearing lack the ability to regenerate, which has limited treatments for loss. Current regenerative medicine strategies have focused on transplanting stem or genetic manipulation of surrounding support in inner ear encourage replacement damaged correct Yet, extracellular matrix (ECM) may play a vital role inducing and maintaining function cells, not been well investigated. Using cochlear ECM as scaffold grow adult provide unique insights...

Abstract The steroid dexamethasone is used intraoperatively to prevent postoperative nausea. Studies of intraoperative use in diabetic patients have shown conflicting effects on blood glucose and complications, their has not yet been studied the burn population. A review adult acute undergoing surgery at a verified center from 2012 2017 was conducted. Statistical analysis compared those who did receive an steroid. total 74 underwent 121 operations were identified; administered 14.0% cases....

Negative pressure wound therapy (NPWT) is used clinically to promote tissue formation and closure. In this study, a porcine model was further investigate the mechanisms as how NPWT modulates healing via utilization of form called vacuum-assisted To observe effect more accurately, non-NPWT control wounds containing GranuFoam™ dressings, without vacuum exposure, were utilized. situ histological analysis revealed that enhanced plasma protein adsorption throughout GranuFoam™, resulting in...

Abstract: Advancements in high content image analysis have led to an increase the adoption of these techniques basic science and clinical research. High-throughput approaches imaging require minimal user interventions, circumventing often prohibitively time-consuming unreliable standard manual analysis. In this study, we demonstrate how (HCI) techniques, combination with (HCA), can be paired more traditional quantify both micro- macro-level features biopsied tissue sections. High-resolution,...

Tissue engineering and gene therapy strategies offer new ways to repair permanent damage mechanosensory hair cells (MHCs) by differentiating human Wharton’s Jelly (HWJCs). Conventionally, these require the classification of each cell as differentiated or undifferentiated. Automated tools, however, may serve a novel method rapidly classify cells. In this paper, images from previous work, where HWJCs were into MHC-like cells, examined. Various features extracted images, those which pertinent...

Introduction: Wound therapies are capable of modulating the complex molecular signaling profile tissue regeneration. However traditional, bulk analysis results in nonspecific expressional profiles and diluted that lacks temporal-spatial information. Methods: An acute incisional porcine wound model was developed context negative pressure therapy (NPWT). Dressing materials were inserted into wounds with or without NPWT exposure evaluated over 8-hours. Upon explantation, stratified dissected...