Abstract The natural polymers chitosan and alginate represent an attractive material choice for biodegradable inplants. These were used as coating materials to make positively negatively charged PLGA nanoparticles, respectively. After blending at total solids concentration >10% wt/vol, these oppositely nanoparticles yielded a cohesive colloidal gel. Electrostatic forces between produced stable 3D porous network that may be extruded or molded the desired shape. This high system...

The temporomandibular joint (TMJ) presents many problems in modern musculoskeletal medicine. Patients who suffer from TMJ disorders often experience a major loss quality of life due to the debilitating effects that can have on everyday activities. Cartilage tissue engineering lead replacement tissues could be used treat disorders. In this study, spinner flask was for period 6 days seed polyglycolic acid (PGA) scaffolds with either condylar chondrocytes or mesenchymal-like stem cells derived...

Hydrogel surface properties can be modified to form bioactive interfaces modulate the osteogenic differentiation of stem cells. In this work, a hydrogel made gelatin methacrylamide (GelMA) and alginate was designed tested as scaffold control stem-cell differentiation. The hydrogel's treated with polydopamine (pDA) create an adhesive layer for adsorption osteoinductive drug dexamethasone (Dex). presence pDA coating enhanced Dex retention over 21 days. This effect resulted in delay hASCs...

Three-dimensional (3D) bioprinting holds the promise to fabricate tissue and organ substitutes for regenerative medicine. However, lack of bioactive inks support functional living constructs is one main limitations hindering progress this technology. In study, a biofunctional human-based nanocomposite bioink (HUink) composed platelet lysate hydrogels reinforced by cellulose nanocrystals reported. When combined with suspended technologies, HUink allows biofabrication 3D freeform high...

Despite the significant morbidity associated with temporomandibular joint (TMJ), little is known about pathophysiology of this complex joint. TMJ disc degeneration plays a central role in progression disorders, and therefore regeneration would be crucial treatment modality. Unfortunately, scarce information structural functional characteristics available. The current study aims to provide standard for biomechanical behavior future tissue engineering studies. was loaded under uniaxial tension...

Cells derived from Wharton's jelly human umbilical cords (called cord mesenchymal stromal cells herein) are a novel cell source for musculoskeletal tissue engineering. In this study, we examined the effects of different seeding densities on efficiency, proliferation, biosynthesis, mechanical integrity, and chondrogenic differentiation. were seeded non-woven polyglycolic acid (PGA) meshes in an orbital shaker at 5, 25, or 50 million cells/mL then statically cultured 4 weeks medium. At week 0,...

Scaffolds made from cartilage extracellular matrix are promising materials for articular repair, attributed to their intrinsic bioactivity that may promote chondrogenesis. While several matrix-based scaffolds have supported chondrogenesis

Abstract Background Cartilage injuries pose significant challenges in horses and often lead to post-traumatic osteoarthritis (PTOA). Despite the advances surgical regenerative techniques, result most cases is formation of a fibrocartilage repair tissue. Cell-based cartilage therapies are mainly focused on equine bone marrow-derived mesenchymal stem cells (eBMSCs) as they easily accessible, multipotent. Nonetheless, alternative allogeneic sources, for example umbilical cord matrix stromal...

Tracheal stenosis can become a fatal condition, and current treatments include augmentation of the airway with autologous tissue. A tissue-engineered approach would not require donor source, while providing an implant that meets both surgeons' patients' needs. fibrous, polymeric scaffold organized in gradient bilayers polycaprolactone (PCL) poly-lactic-co-glycolic acid (PLGA) 3D printed structural ring supports, inspired by native trachea rings, could meet this need. The purpose study was to...

There is growing evidence indicating the need to combine rehabilitation and regenerative medicine fields maximize functional recovery after spinal cord injury (SCI), but there are limited methods synergistically fields. Conductive biomaterials may enable synergistic combination of with electric stimulation (ES), which direct ES neurons enhance axon regeneration reorganization for better recovery; however, three major challenges in developing conductive biomaterials: (1) low conductivity...

This objective of this study was to determine the effects a rotating bioreactor in temporomandibular joint (TMJ) disc tissue engineering. Porcine TMJ cells were seeded at density 20 million cells/mL onto nonwoven poly(glycolic acid) (PGA) scaffolds spinner flasks for 1 week and then cultured either under static conditions or period 6 weeks. A series analyses performed, including mechanical testing, measurement cellularity, quantification matrix biosynthesis with hydroxyproline assay...

Tracheal stenosis caused by congenital anomalies, tumors, trauma, or intubation-related damage can cause severe breathing issues, diminishing the quality of life, and potentially becoming fatal. Current treatment methods include laryngotracheal reconstruction slide tracheoplasty. Laryngotracheal utilizes rib cartilage harvested from patient, requiring a second surgical site. Slide tracheoplasty involves complex procedure to splay open trachea reconnect both segments widen lumen. A clear need...

Human umbilical cord mesenchymal stromal cells (hUCMSCs) are an attractive cell source for tissue engineering with numerous advantages over other adult stem sources, such as great expansion ability in vitro and extensive availability. The objective of this 6-week study was to test the hypothesis that switching from chondrogenic transforming growth factor-beta3 (TGF-beta3) anabolic insulin-like factor-I (IGF-I) at 3-week time point would produce more cartilage-like matrix than TGF-beta3...

The temporomandibular joint (TMJ) is a site of intense morbidity for millions people, especially young, pre-menopausal women. Central to TMJ afflictions are the cartilaginous tissues TMJ, es

In the treatment of severe traumatic brain injury (TBI), decompressive craniectomy is commonly used to remove a large portion calvarial bone allow unimpeded swelling. Hydrogels have potential revolutionize TBI by permitting single-surgical intervention, remaining pliable during swelling, and tuned regenerate after swelling has subsided. With this motivation, our goal present material capable regenerating across critical size defect. We therefore proposed use methacrylated solubilized...

ABSTRACT This study develops a simple copolymerization/crosslinking technique to control the swelling and mechanical properties of hyaluronic acid‐based hydrogels. Because widespread acceptance poly(ethylene glycol) in biomedical applications, functionalized oligomers ethylene glycol (EG) were used as comonomers crosslink methacrylated acid (MHA). The degree, shear elastic moduli, fracture (stress strain) gels investigated function crosslinking oligomer length reactive group(s). It was...

The potential chondroinductivity from cartilage matrix makes it promising for repair; however, matrix-based hydrogels developed thus far have failed to match the mechanical performance of native or be bioprinted without adding polymers reinforcement. There is a need with robust and paste-like precursor rheology bioprinting/enhanced surgical placement. In current study, our goals were increase hydrogel stiffness develop precursor/printability methacryl-modified solubilized devitalized...

Tracheal stenosis is a life-threatening disease and current treatments include surgical reconstruction with autologous rib cartilage the highly complex slide tracheoplasty technique. We propose using sustainable implant, composed of tunable, fibrous scaffold encapsulated chondrogenic growth factor (transforming factor-beta3 [TGF-β3]) or seeded allogeneic rabbit bone marrow mesenchymal stromal cells (BMSCs). In vivo functionality these constructs was determined by implanting them in induced...