A5007514272- Ruminant Nutrition and Digestive Physiology
- Mesenchymal stem cell research
- Ion channel regulation and function
- Pluripotent Stem Cells Research
- Muscle metabolism and nutrition
- Neuroscience and Neuropharmacology Research
- Tissue Engineering and Regenerative Medicine
- Cardiac electrophysiology and arrhythmias
- Plant Stress Responses and Tolerance
- Fossil Insects in Amber
- Cystic Fibrosis Research Advances
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Genetic and phenotypic traits in livestock
- Coleoptera Taxonomy and Distribution
- Insect and Arachnid Ecology and Behavior
- Osteoarthritis Treatment and Mechanisms
- Receptor Mechanisms and Signaling
- Microbial Metabolic Engineering and Bioproduction
- Xenotransplantation and immune response
- Ion Transport and Channel Regulation
- Fatty Acid Research and Health
- Neurogenesis and neuroplasticity mechanisms
- Anaerobic Digestion and Biogas Production
- Plant-Microbe Interactions and Immunity
Elanco (United States)
2023-2024
The Ohio State University
2020-2023
University of California, Davis
2016
Nottingham Trent University
2015
Imperial College London
2008-2014
Morgan Stanley Children's Hospital
2010
Writtle University College
2010
Columbia University
2010
University of Kansas
2005-2008
Research International (United States)
2008
We have identified an easily attainable source of primitive, potentially multipotent stem cells from Wharton's jelly, the matrix umbilical cord. jelly been propagated in culture for more than 80 population doublings. Several markers cells, including c-kit (CD117), and telomerase activity are expressed these cells. Treatment with basic fibroblast growth factor overnight low-serum media plus butylated hydroxyanisole dimethylsulfoxide induced to express a neural phenotype. Within several hours...
Abstract Receptor-like proteins (RLPs) are cell surface receptors that typically consist of an extracellular leucine-rich repeat domain, a transmembrane and short cytoplasmatic tail. In several plant species, RLPs have been found to play role in disease resistance, such as the tomato (Solanum lycopersicum) Cf Ve apple (Malus domestica) HcrVf2 protein mediate resistance against fungal pathogens Cladosporium fulvum, Verticillium spp., Venturia inaequalis, respectively. addition, development;...
The temporomandibular joint (TMJ) presents many problems in modern musculoskeletal medicine. Patients who suffer from TMJ disorders often experience a major loss quality of life due to the debilitating effects that can have on everyday activities. Cartilage tissue engineering lead replacement tissues could be used treat disorders. In this study, spinner flask was for period 6 days seed polyglycolic acid (PGA) scaffolds with either condylar chondrocytes or mesenchymal-like stem cells derived...
Two bacterial isolates, designated AMG-D1Tand AMG-D2, were recovered from 25·35-million-year-old Dominican amber. AMG-D2 biochemically most closely resemble Staphylococcus xylosus; they differ physiologically other staphylococci. Fatty acid analysis and comparisons with extensive databases unable to show relatedness any specific taxon. Moreover, contain tuberculostearic meso-diaminopimelic acid, characteristic of the G+C-rich coryneform bacteria, as opposed l-lysine have a G+C ratio 35 mol%....
Three experiments assessed branched-chain volatile fatty acid (BCVFA) stimulation of neutral detergent fiber (NDF) disappearance after 24 h incubation in batch cultures derived from ruminal fluid inocula that were enriched with particulate-phase bacteria. In experiment 1, a control was compared 3 treatments isomolar doses all BCVFA (plus valerate), AA (BCAA), or half each and BCAA mix either alfalfa grass hays (50%) ground corn grain (50%). A portion the 13C, valerate (also 13C) added BCVFA....
To maintain membrane homeostasis, ruminal bacteria synthesize branched-chain fatty acids (BCFA) or their derivatives (vinyl ethers) that are recovered during methylation procedures as aldehydes (BCALD). Many strains of cellulolytic require 1 more volatile acid (BCVFA). Therefore, the objective this study was to investigate BCVFA incorporation into bacterial lipids under different dietary conditions. The an incomplete block design with 8 continuous culture fermenters used in 4 periods...
Abstract Potential therapeutic effects of Oct-4-positive rat umbilical cord matrix (RUCM) cells in treating cerebral global ischemia were evaluated using a reproducible model cardiac arrest (CA) and resuscitation rats. Animals randomly assigned to four groups: A, sham-operated; B, 8-minute CA without pretreatment; C, pretreated with defined media; D, Oct-4+ RUCM cells. Pretreatment was done 3 days before by 2.5-μl microinjection media or approximately 104 left thalamic nucleus, hippocampus,...
Branched-chain amino acids are deaminated by amylolytic bacteria to branched-chain volatile fatty (BCVFA), which growth factors for cellulolytic bacteria. Our objective was determine the dietary conditions that would increase uptake of BCVFA rumen We hypothesized increased forage bacterial abundance and incorporation into their structure. Supplemental polyunsaturated acids, supplied via corn oil (CO), should inhibit growth, but we additional alleviate inhibition. Further, supplemental...
In dogs and in humans, potassium channels formed by ether-a-go-go-related gene 1 protein ERG1 (KCNH2) KCNQ1 α-subunits, association with KCNE β-subunits, play a role normal repolarization may contribute to abnormal associated long QT syndrome (LQTS). The molecular basis of horse heart is unknown, although horses exhibit common cardiac arrhythmias receive drugs that induce LQTS. heart, we have used immunoblotting immunostaining demonstrate the expression ERG1, KCNQ1, KCNE1, KCNE3 proteins...
Restoration of chloride conductance via the introduction an anion selective pore, formed by a channel-forming peptide, has been hypothesized as novel treatment modality for patients with cystic fibrosis (CF). Delivery these peptide sequences to airway cells from aqueous environment in absence organic solvents is paramount. New highly soluble COOH- and NH(2)-terminal truncated peptides, derived second transmembrane segment glycine receptor alpha-subunit (M2GlyR), were generated, decreasing...
To support improving genetic potential for increased milk production, intake of digestible carbohydrate must also increase to provide energy and microbial protein synthesis. We hypothesized that the provision exogenous branched-chain volatile fatty acids (BCVFA) would improve both neutral detergent fiber (NDF) degradability efficiency However, BCVFA should be more beneficial with increasing bacterial synthesis associated passage rate (kp). decreasing pH need isobutyrate over...
Some cellulolytic bacteria require 1 or more branched-chain volatile fatty acids (BCVFA) for the synthesis of AA and long-chain because they are not able to uptake lack enzymes synthesize de novo. Supplemental BCVFA valerate were included previously as a feed additive that was later removed from market; these older studies current have noted improvements in neutral detergent fiber digestibility milk efficiency. However, most provided single else isobutyrate (IB), 2-methylbutyrate (MB),...
Some cellulolytic bacteria cannot transport branched-chain AA (BCAA) and do not express complete synthesis pathways, thus depending on cross-feeding for volatile fatty acid (BCVFA) precursors membrane lipids or reductive carboxylation to BCAA. Our objective was assess BCVFA uptake BCAA in continuous cultures administered high forage (HF) low (LF) diets without with corn oil (CO). We hypothesized that would be used more the HF than LF diets. To help overcome bacterial inhibition by...
This literature review explains current understanding of the mechanisms by which branched-chain volatile fatty acids (BCVFA), historically termed isoacids, can improve fiber digestibility, microbial protein production, and either milk production or efficiency. Peer-reviewed ADSA abstracts were primary course information reviewed. Although traditionally included in isoacid supplements with 3 BCVFA, straight-chain valerate does not warrant being included. Because high concentration its...
Comparative molecular field analysis (CoMFA) was used to analyze the relationship between structure of a group ryanoids and modulation calcium channel function ryanodine receptor. The conductance properties receptors purified from sheep heart were measured using planar, lipid bilayer technique. magnitude ryanoid-induced fractional strongly correlated specific structural loci on ligand. Briefly, electrostatic effects more prominent than steric effects. 10-position ryanoid had greatest...
The experiment was conducted to understand ruminal effects of diet modification during moderate milk fat depression (MFD) and 2-hydroxy-4-(methylthio)-butanoic acid (HMTBa) isoacids on alleviating MFD. Five ruminally cannulated cows were used in a 5 × Latin square design with the following dietary treatments (dry matter basis): high-forage low-starch control 1.5% safflower oil (HF-C); low-forage high-starch (LF-C); LF-C supplemented HMTBa (0.11%; 28 g/d; LF-HMTBa); [(IA) 0.24%; 60 LF-IA]; IA...
Our objectives were to evaluate potential interactions in culture conditions that influence how exogenously dosed branched-chain VFA (BCVFA) would be recovered as elongated fatty acids (FA) or affect bacterial populations. A 2 × factorial arrangement of treatments evaluated 3 factors: (1) without versus with BCVFA (0 vs. mmol/d each isobutyrate, isovalerate, and 2-methylbutyrate; dose was partially substituted 13C-enriched tracers before during the collection period); (2) high low pH...