A5038991494- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Anesthesia and Neurotoxicity Research
- Mass Spectrometry Techniques and Applications
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced MRI Techniques and Applications
- Antibiotic Resistance in Bacteria
- Advanced NMR Techniques and Applications
- Cardiac Arrest and Resuscitation
- Neonatal and fetal brain pathology
- Cannabis and Cannabinoid Research
- Enzyme Structure and Function
- Crystallography and molecular interactions
- Chemical Thermodynamics and Molecular Structure
- Cardiac Ischemia and Reperfusion
- Anesthesia and Sedative Agents
- Neurobiology and Insect Physiology Research
- Atomic and Subatomic Physics Research
- Metabolomics and Mass Spectrometry Studies
- Electron Spin Resonance Studies
University of Pittsburgh
2015-2024
Shanghai Jiao Tong University
2016-2024
University of Warsaw
2023
Jiangnan University
2023
Nanjing Agricultural University
2022-2023
Stanford University
2002-2022
Shaanxi University of Technology
2022
Second Affiliated Hospital of Zhejiang University
2016-2022
Zhejiang University
2022
Changchun University of Chinese Medicine
2022
Certain types of nonpsychoactive cannabinoids can potentiate glycine receptors (GlyRs), an important target for nociceptive regulation at the spinal level. However, little is known about potential and mechanism glycinergic chronic pain treatment. We report that systemic intrathecal administration cannabidiol (CBD), a major component marijuana, its modified derivatives significantly suppress inflammatory neuropathic without causing apparent analgesic tolerance in rodents. The currents dorsal...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSmall-volume voltammetric detection of 4-aminophenol with interdigitated array electrodes and its application to electrochemical enzyme immunoassayOsamu. Niwa, Yan. Xu, H. Brian. Halsall, William R. HeinemanCite this: Anal. Chem. 1993, 65, 11, 1559–1563Publication Date (Print):June 1, 1993Publication History Published online1 May 2002Published inissue 1 June 1993https://doi.org/10.1021/ac00059a013RIGHTS & PERMISSIONSArticle...
Rapid response to witnessed, pulseless cardiac arrest is associated with increased survival.Assessment of arrests occurring at 538 hospitals during a 9-yr period indicates that CPR did not occur immediately 0 min in 5.7% patients despite guidelines for instantaneous initiation. Delay initiation was significantly decreased survival.Time and subsequent time administration defibrillation shock (for shockable arrhythmias) epinephrine were both reduced patient survival.Because the extent which...
Inhibition of N-methyl-d-aspartate (NMDA) receptors has been viewed as a primary cause xenon anesthesia, yet the mechanism is unclear. Here, we investigated interactions between and ligand-binding domain (LBD) NMDA receptor examined xenon-induced structural dynamical changes that are relevant to functional receptor. Several comparative molecular dynamics simulations were performed on two X-ray structures representing open- closed-cleft LBD We identified plausible action sites in LBD,...
ELIC, the pentameric ligand-gated ion channel from Erwinia chrysanthemi, is a prototype for Cys-loop receptors. Here we show that acetylcholine competitive antagonist ELIC. We determine acetylcholine–ELIC cocrystal structure to 2.9-Å resolution and find binding an aromatic cage at subunit interface induces significant contraction of loop C other structural rearrangements in extracellular domain. The side chain pore-lining residue F247 reorients pore size consequently enlarges, but remains...
Type A γ-aminobutyric acid receptors (GABAARs) are inhibitory pentameric ligand-gated ion channels in the brain. Many anesthetics and neurosteroids act through binding to GABAAR transmembrane domain (TMD), but structural basis of their actions is not well understood no resting-state structure has been determined. Here, we report crystal structures apo neurosteroid anesthetic alphaxalone-bound desensitized chimeric α1GABAAR (ELIC-α1GABAAR). The chimera retains functional pharmacological...
Abstract The intracellular domain (ICD) of Cys-loop receptors mediates diverse functions. To date, no structure a full-length ICD is available due to challenges stemming from its dynamic nature. Here, combining nuclear magnetic resonance (NMR) and electron spin experiments with Rosetta computations, we determine structures the human α7 nicotinic acetylcholine receptor in resting state. We show that ~57% residues are highly flexible regions, primarily large loop (loop L) most mobile segment...
Interactions of volatile anesthetics with the central nervous system are characterized by low yet specific binding affinities. Although neurotransmitter-gated ion channels considered primary anesthetic targets, mechanism action at molecular level remains elusive. We consider here theoretical implications channel dynamics on in a simplified membrane-channel system. Large-scale 2.2-ns all-atom simulations were performed to study effects halothane, clinical anesthetic, gramicidin A (gA) fully...
Neurons communicate with each other dynamically; how such communications lead to consciousness remains unclear. Here, we present a theoretical model understand the dynamic nature of sensory activity and information integration in hierarchical network, which edges are stochastically defined by single parameter p representing percolation probability transmission. We validate comparing transmitted original signal distributions, show that basic version this can reproduce key spectral features...
Abstract Potential therapeutic effects of Oct-4-positive rat umbilical cord matrix (RUCM) cells in treating cerebral global ischemia were evaluated using a reproducible model cardiac arrest (CA) and resuscitation rats. Animals randomly assigned to four groups: A, sham-operated; B, 8-minute CA without pretreatment; C, pretreated with defined media; D, Oct-4+ RUCM cells. Pretreatment was done 3 days before by 2.5-μl microinjection media or approximately 104 left thalamic nucleus, hippocampus,...
ABSTRACT β-Lactamases are important antibiotic resistance determinants expressed by bacteria. By studying the mechanistic properties of β-lactamases, we can identify opportunities to circumvent through design novel inhibitors. Comparative amino acid sequence analysis class A β-lactamases reveals that many enzymes possess a localized positively charged residue (e.g., R220, R244, or R276) is critical for interactions with β-lactams and β-lactamase To better understand contribution these...