A5011788151- Gut microbiota and health
- Probiotics and Fermented Foods
- Paleopathology and ancient diseases
- Archaeology and ancient environmental studies
- Forensic and Genetic Research
- Insect and Arachnid Ecology and Behavior
- Microbial Metabolites in Food Biotechnology
- Fossil Insects in Amber
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Plant and animal studies
- Environmental DNA in Biodiversity Studies
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Forensic Anthropology and Bioarchaeology Studies
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Molecular Biology Techniques and Applications
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Biosensors and Analytical Detection
- Oral microbiology and periodontitis research
- Bacillus and Francisella bacterial research
- Coleoptera Taxonomy and Distribution
- Reproductive tract infections research
California Polytechnic State University
2013-2025
University of Havana
2023
Predictive Biology (United States)
2019-2023
American Type Culture Collection
2018
North Carolina State University
2002-2005
Biotechnology Institute
2001-2003
University of Nebraska–Lincoln
2003
Washington State University
1974
University of Montana
1974
A bacterial spore was revived, cultured, and identified from the abdominal contents of extinct bees preserved for 25 to 40 million years in buried Dominican amber. Rigorous surface decontamination amber aseptic procedures were used during recovery bacterium. Several lines evidence indicated that isolated bacterium ancient origin not an extant contaminant. The characteristic enzymatic, biochemical, 16S ribosomal DNA profiles is most closely related Bacillus sphaericus.
Lactobacillus acidophilus NCFM is a probiotic bacterium that has been produced commercially since 1972. The complete genome 1,993,564 nt and devoid of plasmids. average GC content 34.71% with 1,864 predicted ORFs, which 72.5% were functionally classified. Nine phage-related integrases predicted, but no prophages found. However, three unique regions designated as potential autonomous units (PAUs) identified. These resemble structure bear characteristics both plasmids phages. Analysis the PAUs...
The extensive use of glyphosate-based herbicides has raised concerns about its impact on soil health and microbial communities, particularly due to the persistence AMPA. This study evaluates effects PaleoPower, a co-fermented inoculant, composition, diversity, glyphosate degradation in cotton field. applied at 1.6 × 10⁸ CFU per square meter, introduced eight bacterial strains, bioactive postbiotics, prebiotics into soil. Post-harvest analyses revealed reductions AMPA levels 72%...
Lactobacillus acidophilus is a probiotic organism that displays the ability to use prebiotic compounds such as fructooligosaccharides (FOS), which stimulate growth of beneficial commensals in gastrointestinal tract. However, little known about mechanisms and genes involved FOS utilization by species. Analysis L. NCFM genome revealed an msm locus composed transcriptional regulator Lac I family, four-component ATP-binding cassette (ABC) transport system, fructosidase, sucrose phosphorylase....
The process of natural mummification is a rare and unique from which little known about the resulting microbial community structure. In present study, we characterized microbiome paleofeces, ascending, transverse descending colon an 11th century A.D. pre-Columbian Andean mummy by 16S rRNA gene high-throughput sequencing metagenomics. Firmicutes were most abundant bacterial group, with Clostridium spp. comprising up to 96.2% mummified gut, while Turicibacter represented 89.2% bacteria...
Abstract Several studies have documented the significant impact of methodological choices in microbiome analyses. The myriad options available complicate replication results and generally limit comparability findings between independent that use differing techniques measurement pipelines. Here we describe Mosaic Standards Challenge (MSC), an international interlaboratory study designed to assess variables on results. MSC did not prescribe methods but rather asked participating labs analyze 7...
ABSTRACT Amino acid decarboxylation-antiporter reactions are one of the most important systems for maintaining intracellular pH between physiological limits under stress. We analyzed Lactobacillus acidophilus NCFM complete genome sequence and selected four open reading frames with similarities to genes involved decarboxylation in tolerance several microorganisms. Putative encoding an ornithine decarboxylase, amino permease, a glutamate γ-aminobutyrate antiporter, transcriptional regulator...
ABSTRACT Two genes, bshA and bshB , encoding bile salt hydrolase enzymes (EC 3.5.1.24) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation these genes via chromosomal insertion an integration vector demonstrated different substrate specificities for two enzymes.
Coprolites are fossilized feces that can be used to provide information on the composition of intestinal microbiota and, as we show, possibly diet. We analyzed human coprolites from Huecoid and Saladoid cultures a settlement Vieques Island, Puerto Rico. While more is known about culture, it believed both societies co-existed this island approximately 5 1170 AD. By extracting DNA coprolites, followed by metagenomic characterization, show distinguished each other basis their bacterial fungal...
Two bacterial isolates, designated AMG-D1Tand AMG-D2, were recovered from 25·35-million-year-old Dominican amber. AMG-D2 biochemically most closely resemble Staphylococcus xylosus; they differ physiologically other staphylococci. Fatty acid analysis and comparisons with extensive databases unable to show relatedness any specific taxon. Moreover, contain tuberculostearic meso-diaminopimelic acid, characteristic of the G+C-rich coryneform bacteria, as opposed l-lysine have a G+C ratio 35 mol%....
We report here the isolation of DNA from abdominal tissue four extinct stingless bees (Proplebeia dominicana) in Dominican amber, PCR amplification a 546-bp fragment 16S rRNA gene Bacillus spp., and their corresponding nucleotide sequences. These sequences were used basic local alignment search tool searches nonredundant nucleic acid data bases, highest scores obtained with spp. Phylogenetic inference analysis by maximum-likelihood method revealed close phylogenetic relationships presumed...
Dysbiosis of the intestinal microbiota has been shown to result in altered immune responses and increased susceptibility infection; as such, state microbiome may have profound implications perioperative setting. In this first-in-class study, we used 16s ribosomal RNA sequencing analysis a mouse model general anesthesia investigate effects volatile anesthetics on diversity composition microbiome. After 4-hour exposure isoflurane, observed decrease bacterial diversity. Taxonomic alterations...
Characterization of naturally mummified human gut remains could potentially provide insights into the preservation and evolution commensal pathogenic microorganisms, metabolic profiles. We characterized microbiome two pre-Columbian Andean mummies dating to 10-15th centuries using 16S rRNA gene high-throughput sequencing metagenomics, compared them a previously an 11th century AD mummy. Our previous study showed that Clostridiales represented majority bacterial communities in remains, but...
Journal Article Factors Affecting Bacillus thuringiensis Total Field Persistence Get access K. L. H. Leong, Leong Biological Sciences Department, California Polytechnic State University, San Luis Obispo, CA 93407 Search for other works by this author on: Oxford Academic PubMed Google Scholar R. J. Cano, Cano A. M. Kubinski Environmental Entomology, Volume 9, Issue 5, 1 October 1980, Pages 593–599, https://doi.org/10.1093/ee/9.5.593 Published: 01 1980 history Received: 27 November 1979
The study of coprolites from earlier cultures represents a great opportunity to an "unaltered" composition the intestinal microbiota. To test this, pre-Columbian two cultures, Huecoid and Saladoid, were evaluated for presence DNA, proteins lipids by cytochemical staining, human and/or dog-specific Bacteroides spp. PCR, as well bacteria, fungi archaea using Terminal Restriction Fragment analyses. lipids, human-specific DNA detected in all coprolites. Multidimensional scaling analyses resulted...
The male human body found in an Alpine glacier on September 19, 1991 ("Tyrolean Iceman") has, for the first time history, given scientists a chance to perform detailed anatomical, histological, and molecular investigations organs of person from Neolithic Age (5350–5100 B.P.). In present study, tissue samples aseptically taken stomach colon mummy were utilized DNA extraction, was PCR-amplified, using primer pairs designed bind fragments 16s ribosomal RNA gene (16s rDNA) broad range bacteria....