A5075931648- SARS-CoV-2 detection and testing
- Dermatology and Skin Diseases
- SARS-CoV-2 and COVID-19 Research
- Acute Myeloid Leukemia Research
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Bone Metabolism and Diseases
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Mitochondrial Function and Pathology
- Bone health and treatments
- Advancements in Transdermal Drug Delivery
- Skin Protection and Aging
- Hair Growth and Disorders
- Histone Deacetylase Inhibitors Research
- Chronic Myeloid Leukemia Treatments
- Homelessness and Social Issues
- ATP Synthase and ATPases Research
- COVID-19 Clinical Research Studies
- Hedgehog Signaling Pathway Studies
- Kruppel-like factors research
- Immune Cell Function and Interaction
- Bacterial biofilms and quorum sensing
- Genomics, phytochemicals, and oxidative stress
- Infection Control and Ventilation
Charité - Universitätsmedizin Berlin
2011-2024
Vivantes Klinikum
2020-2024
Labor (Italy)
2021
Universitätsklinikum Würzburg
2014
Humboldt-Universität zu Berlin
2014
Max Delbrück Center
2000-2007
Universität Hamburg
1985
Two elementary parameters for quantifying viral infection and shedding are load whether samples yield a replicating virus isolate in cell culture. We examined 25,381 cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Germany, including 6110 from test centers attended by presymptomatic, asymptomatic, mildly symptomatic (PAMS) subjects, 9519 who were hospitalized, 1533 B.1.1.7 lineage infections. The the youngest subjects was lower than that older 0.5 (or fewer) log
Mitochondria are dynamic organelles that change morphology by controlled fission and fusion events. Mitochondrial is regulated a conserved protein complex assembled at the outer membrane. Human MTP18 novel nuclear-encoded mitochondrial membrane protein, implicated in controlling fission. Upon overexpression of MTP18, was altered from filamentous to punctate structures suggesting excessive fragmentation blocked cells coexpressing either Mfn1 or Drp1K38A, dominant negative version Drp1. Also,...
Abstract Several studies have documented the significant impact of methodological choices in microbiome analyses. The myriad options available complicate replication results and generally limit comparability findings between independent that use differing techniques measurement pipelines. Here we describe Mosaic Standards Challenge (MSC), an international interlaboratory study designed to assess variables on results. MSC did not prescribe methods but rather asked participating labs analyze 7...
Evolving SARS-CoV-2 variants and changing levels of pre-existing immunity require re-evaluation antigen-detecting rapid diagnostic test (Ag-RDT) performance. We investigated possible associations between Ag-RDT sensitivity various potential influencing factors, such as immunisation status viral variant, in symptomatic hospital employees.
We identified a novel human cDNA encoding mitochondrial protein, MTP18 (mitochondrial 18 kDa) as transcriptional downstream target of phosphatidylinositol (PI) 3-kinase signaling. demonstrate that mRNA well protein expression is dependent on PI activity. Confocal microscopy and biochemical fractionation revealed localization MTP18. Loss-of-function analysis employing antisense molecules essential for cell viability in PC-3 HaCaT cells. show knockdown level results cytochrome c release from...
As in higher vertebrates, stress increases the susceptibility of fishes to disease.This effect is accompanied by changes circulating white blood cells: number lymphocytes decreases while granulocytes increases.Investigations were therefore undertaken determine what ways induces hemopoietic organs, particularly spleen and head kidneys rainbow trout Salmo gairdneri Handling social conflict used as stressors.These psychic stimuli are typically encountered aquaculture under laboratory...
Multiple myeloma (MM) is a lethal human cancer characterized by clonal expansion of malignant plasma cells in bone marrow. Mouse models MM are technically challenging and do not always recapitulate disease. Therefore, new mouse for needed. Mineral-oil induced plasmacytomas (MOPC) develop the peritoneal cavity oil-injected BALB/c mice. However, MOPC typically grow extramedullary considered poor MM. Here we describe an vivo-selected MOPC315 variant, called MOPC315.BM, which can be maintained...
Despite evidence that deregulated Notch signalling is a master regulator of multiple myeloma (MM) pathogenesis, its contribution to bone disease remains be resolved. promotes survival human MM cells and triggers osteoclast activity in vitro. Here, we show inhibition through the γ-secretase inhibitor XII (GSI XII) induces apoptosis murine MOPC315.BM with high activity. GSI impairs differentiation receptor activator NF-κB ligand (RANKL)-stimulated RAW264.7 In model has potent anti-MM reduces...
Skin microbiome and skin physiology are important indicators of the epidermal homeostasis status. Stress models can reveal pathological conditions modulating effects. Here we investigated cutaneous in relation to after mild tape stripping (TS) without treatment compared two cosmetic leave-on lotions (pH 5.5 vs. pH 9.3) 25 healthy volunteers.The was analyzed by 16S-rRNA-gene amplicon sequencing put following parameter: barrier function (TEWA-Meter TM300), stratum corneum hydration...
Evolving SARS-CoV-2 variants and changing levels of pre-existing immunity require re-evaluation rapid antigen test performance. In hospital employees in Berlin, Germany, from late 2020 to early 2022, we found decreasing sensitivity over time, 80% 67%, despite a 5·6 fold increase median viral RNA concentrations symptomatic their infection course. These changes were observed parallel with sharp the percentage who at least doubly vaccinated or recovered infection. Based on statistical analyses...
Living conditions in homeless shelters facilitate the transmission of COVID-19. Social determinants and pre-existing health place people at increased risk severe disease. Described outbreaks resulted high proportions infected residents staff members. In addition to other infection prevention strategies, regular shelter-wide (universal) testing for COVID-19 may be valuable, depending on level community when resources permit.This was a prospective feasibility cohort study evaluate universal...
A better understanding of events triggering chronic myeloid leukemia progression is critical for optimized clinical management (CML). We sought to validate that increased expression Musashi 2 (MSI2), a post-transcription regulator, associated with and prognosis. Screening 152 patients CML showed MSI2 was significantly decreased among in phase (CP) at diagnosis (p < 0.0001), but found no significant difference between the normal control group treated CP. Moreover 0.0001) advance disease (AD)...
Abstract Background Several studies have documented the significant impact of methodological choices in microbiome analyses. The myriad options available complicate replication results and generally limit comparability findings between independent that use differing techniques measurement pipelines. Here we describe Mosaic Standards Challenge (MSC), an international interlaboratory study designed to assess variables on results. MSC did not prescribe methods but rather asked participating...
The upper follicular compartment, a well-known reservoir of cutaneous microbiota, constitutes space for intensive cross-barrier dialogue. lower follicle comprises the bulb and bulge, structures with relative immune-privileged status, crucial physiological cycling, widely considered to be microbial-free. Following our initial immunohistochemical screening regulatory cytokines defensin expression in anagen hair follicles, we aimed confirm results follow-up ELISA investigation. We postulated...
Abstract Introduction Stratum corneum (SC) is essential for skin barrier function, mitigating water loss and shielding against potentially harmful substances allergens. The SC's lipid matrix, arranged in a lamellar structure, integral to its protective role. Our study explores the restoration effects of multilamellar cream with an acidic pH compared basic placebo on physiology interaction microbiome after stress induction via tape stripping (TS). Materials Methods In this double‐blind study,...
Despite their distinct clinical manifestation, frontal fibrosing alopecia (FFA) and lichen planopilaris (LPP) display similar histopathologic features. Aberrant innate immune responses to endogenous or exogenous triggers have been discussed as factors that could drive inflammatory cascades the collapse of stem cell niche. In this exploratory study, we investigate bacterial composition scalp skin plucked hair follicles (HF) patients with FFA, LPP areata circumscripta (AAc), well healthy...
ABSTRACT Background SARS-CoV-2 causes COVID-19, which can be fatal and is responsible for a global pandemic. Variants with increased transmissibility or the potential to evade immunity have emerged represent threat pandemic control. of concern (VOC) identified by sequencing viral RNA, more rapid methods detection subsets signature mutations. Methods We developed multiplex, real-time RT-PCR assay (cobas ® Variant Set 1) qualitative differentiation three key mutations in spike protein: del...