A5049758791- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Whipple's Disease and Interleukins
- Oral microbiology and periodontitis research
- Bacterial biofilms and quorum sensing
- Orthopedic Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- HIV/AIDS oral health manifestations
- Pediatric health and respiratory diseases
- Oral Health Pathology and Treatment
- Vector-borne infectious diseases
- Syphilis Diagnosis and Treatment
- Bartonella species infections research
- Antimicrobial Peptides and Activities
- Tracheal and airway disorders
- Infectious Aortic and Vascular Conditions
- Surgical site infection prevention
- Cystic Fibrosis Research Advances
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Antimicrobial agents and applications
- Erythropoietin and Anemia Treatment
Freie Universität Berlin
2018-2025
Humboldt-Universität zu Berlin
2003-2025
Charité - Universitätsmedizin Berlin
2012-2025
CS Diagnostics
2022-2025
University Hospital Leipzig
2025
Leipzig University
2025
Deutsches Herzzentrum der Charité
2013-2024
Czech Academy of Sciences, Institute of Microbiology
2018-2024
Goethe University Frankfurt
2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2022
Abstract Oral infection of susceptible mice with Toxoplasma gondii results in Th1-type immunopathology the ileum. We investigated gut flora changes during ileitis and determined contributions bacteria to intestinal inflammation. Analysis microflora revealed that was accompanied by increasing bacterial load, decreasing species diversity, translocation. Gram-negative identified as Escherichia coli Bacteroides/Prevotella spp. accumulated inflamed ileum at high concentrations. Prophylactic or...
Whether micro-organisms can live in periapical endodontic lesions of asymptomatic teeth is under debate. The aim the present study was to visualize and identify within directly, using fluorescence situ hybridization (FISH) combination with epifluorescence confocal laser scanning microscopy (CLSM). Thirty-nine were surgically removed, fixed, embedded cold polymerizing resin sectioned. probe EUB 338, specific for domain Bacteria , used together a number species-specific16S rRNA-directed...
Periodontitis is one of the most common chronic inflammatory diseases. A number putative bacterial pathogens have been associated with disease and are used as diagnostic markers. In present study, we compared prevalence oral species in subgingival biofilm generalized aggressive periodontitis (GAP) (n = 44) (CP) 46) patients that a periodontitis-resistant control group (PR) 21). The consisted subjects at least 65 years age only minimal or no history periodontal treatment. total 555 samples...
ABSTRACT “Classical” Whipple's disease (cWD) is caused by Tropheryma whipplei and characterized arthropathy, weight loss, diarrhea. T. infectious endocarditis (TWIE) rarely reported, either in the context of cWD or as isolated TWIE without signs systemic infection. The frequency unknown, systematic studies are lacking. Here, we performed an observational cohort study on incidence infection explanted heart valves two German university centers. Cardiac from 1,135 patients were analyzed for...
Reaction-diffusion models were applied to gain insight into the aspects of biofilm infection and persistence by comparing mathematical simulations with experimental data from varied bacterial biofilms. These comparisons, including three in vitro systems two clinical investigations specimens examined ex vivo, underscored central importance concentration gradients metabolic substrates resulting physiological heterogeneity microorganisms. Relatively simple one-dimensional two-dimensional (2D)...
Abstract Background Bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. These highly dynamic microfloral environments challenge both clinicians and researchers alike. The exploration of structural organisation bacterial interactions within these biofilms is critically important for a thorough understanding disease. In recent years, Filifactor alocis , fastidious, Gram-positive, obligately anaerobic rod was repeatedly identified lesions using...
Classic Whipple disease (CWD) is a systemic infection caused by Tropheryma whipplei. Different diagnostic tools have been developed over the last decades: periodic acid-Schiff (PAS) staining, T whipplei-specific polymerase chain reaction (PCR), and immunohistochemistry (IHC). Despite all these advances, CWD still difficult to diagnose because of variety clinical symptoms possibly long time span between first unspecific full-blown picture disease. Herein, we report an observational cohort...
Fluorescence in situ hybridization (FISH) was performed on sections of plastic-embedded tissue using 16S rRNA-directed oligonucleotide probes to visualize uncultured treponemes skin biopsies cows with digital dermatitis. Plastic as embedding material allowed sectioning hard and soft a defined thickness, avoiding the risk dragging bacteria into while sectioning. Furthermore, it provided good signal-to-noise ratio. Using this method spatial distribution three different bacterial phylotypes...
Background: Whipple disease, which is caused by infection with Tropheryma whipplei, can be treated effectively antimicrobials. Occasionally, inflammation reappears after initial improvement; this often interpreted as refractory or recurrent disease. However, polymerase chain reaction for T. whipplei in tissue sometimes negative during reinflammation, indicating absence of vital bacteria, and reinflammation does not respond to antimicrobials but steroids. Objective: To demonstrate that the...
ABSTRACT Periodontitis, a disease responsible for tooth loss worldwide, is characterized by chronic inflammation of the periodontium, eventually leading to destruction periodontal ligaments and supporting alveolar bone. Spirochetes, identified dark-field microscopy as being most predominant bacteria in advanced lesions, are thought play causative role. Various spirochetal morphotypes were observed, but these yet uncultivable. To assess role organisms we designed oligonucleotide probes...
Treponema denticola and Porphyromonas gingivalis have been identified in atheromatous plaques of two patients suffering from atherosclerosis by PCR fluorescence situ hybridization (FISH). The use the FISH technique suggested that these periodontopathic micro-organisms might be metabolically active within wall arteries, under atherosclerotic lesion.
Background: This study assessed the long‐term effects of adjunctive antimicrobial therapy on periodontal disease progression and oral colonization. Methods: Patients with previously untreated chronic periodontitis subgingival Actinobacillus actinomycetemcomitans and/or Porphyromonas gingivalis were randomly assigned to scaling without (control group) or systemic amoxicillin plus metronidazole CHX irrigation (test group). Relative attachment levels determined mucosal plaque samples taken at...
Retrospective molecular genetic analysis of 166 Mycobacterium intracellulare isolates showed that 143 (86%) strains could be assigned to chimaera sp. nov. Of 97 patients from whom M. was isolated, only 3.3% exhibited mycobacterial lung disease, whereas all caused severe pulmonary infections.