Abstract Background Bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. These highly dynamic microfloral environments challenge both clinicians and researchers alike. The exploration of structural organisation bacterial interactions within these biofilms is critically important for a thorough understanding disease. In recent years, Filifactor alocis , fastidious, Gram-positive, obligately anaerobic rod was repeatedly identified lesions using...

Photoactivated disinfection has a strong local antimicrobial effect. In the field of dentistry it is an emerging adjunct to mechanical debridement during endodontic and periodontal treatment. present study, we investigate effect photoactivated using riboflavin as photosensitizer blue LED light for activation, compare with widely used combination toluidine O red light. Riboflavin highly biocompatible can be activated lamps at hand in dental office. To date, no reports are available on...

To analyse the antibacterial effect of photodynamic therapy (PDT) in combination with various irrigation protocols on a multispecies biofilm root canals ex vivo.A total 160 extracted human single-rooted teeth were divided into four groups (n = 40). In group G1, instrumented up to size 60 (control group), whereas G2 G4 enlarged 40. All inoculated (Enterococcus faecalis, Streptococcus oralis, Prevotella intermedia) for 5 days. G4, instrumentation was performed using 0.9% sodium chloride (NaCl)...

Background Despite continued preventive efforts, dental caries remains the most common disease of man. Organic acids produced by microorganisms in plaque play a crucial role for development carious lesions. During early stages pathogenetic process, repeated pH drops induce changes microbial composition and favour establishment an increasingly acidogenic aciduric microflora. The complex structure biofilms, allowing multitude different ecological environments close proximity, largely...

This study aimed at testing how active and inactive enamel caries lesions differ by their degree of resin infiltration, whether the choice acid pretreatment plays a crucial role. Four examiners assessed 104 human molars premolars with noncavitated classified them as ‘active’ or ‘inactive’ using Nyvad criteria. Forty-five teeth were included in this after independent unanimous lesion activity assessment. Lesions cut perpendicularly into 2 halves. Each half was pretreated either 15%...

ABSTRACT pH in the extracellular matrix of bacterial biofilms is central importance for microbial metabolism. Biofilms possess a complex three-dimensional architecture characterized by chemically different microenvironments close proximity. For decades, measurements have been limited to monitoring bulk with electrodes. Although microelectrodes better spatial resolution developed, they do not permit horizontal gradients real time. Quantitative fluorescence microscopy can overcome these...

Klinisk relevans Orale biofilm bliver patogene på grund af økologiske ændringer, som skyldes værtens kostvaner, mundhygiejne og immunrespons. Sådanne ændringer fører til dysbiose, hvor bakterier dominerer ødelægger det normale gensidige samspil mellem vært mikrobiom. Cariogene fremmer vækst syretolerante mutansstreptokokker laktobaciller. En forøget mængde supragingival nær tandkødsranden gingivitis, men immunrespons spiller en afgørende rolle i inflammationsforløbet. Den dysbiotiske...

Katsaus on tuoreeseen tietoon perustuva yhteenveto terveen suun mikrobiomista ja biofilmeistä karieksen, ientulehduksen parodontiitin osalta. Lisäksi katsauksessa tähdennetään isännän osuutta suusairauksien kehittymisessä. Suunterveyttä ylläpidetään mikrobiomin välisen symbioottisen suhteen avulla. Suun ekosysteemin häiriötekijät, kuten toistuva sokeripitoisten ruokien nauttiminen tai riittämätön omahoito, voivat kuitenkin muuttaa ekologisia olosuhteita, mikä aiheuttaa mikrobiston...

Drescher J, Schlafer S, Schaudinn C, Riep B, Neumann K, Friedmann A, Petrich Göbel UB, Moter A. Molecular epidemiology and spatial distribution of Selenomonas spp. in subgingival biofilms. Eur J Oral Sci 2010; 118: 466–474. © 2010 The aetiology periodontal disease has been a field intensive research the past decades. Along with variety other putative pathogens, different members genus have repeatedly associated both generalized aggressive periodontitis chronic periodontitis. For present...

Extracellular DNA (eDNA) is a major matrix component of many bacterial biofilms. While the presence eDNA and its role in biofilm stability have been demonstrated for several laboratory biofilms oral bacteria, there no data available on function vivo grown dental This study aimed to determine whether was part situ absence sucrose treatment with DNase dispersed 2.5, 5, 7.5, 16.5, or 24 h. Three hundred from 10 participants were collected treated either heat-inactivated 1 The biovolume...

Background and objectivepH in dental biofilms is of central importance for the development caries. We used ratiometric pH-sensitive dye C-SNARF-4 combination with digital image analysis to monitor extracellular pH grown situ without sucrose supply.DesignDental (48 h) from 10 individuals were collected on glass slabs mounted intra-oral appliances. During growth, appliances immersed extra-orally either physiological saline or 4% 2 min, eight times per day. Fluorescence emissions deep layers...

<b><i>Introduction:</i></b> The dental biofilm matrix is an important determinant of virulence for caries development and comprises a variety extracellular polymeric substances that contribute to stability. Enzymes break down components may be promising approach control, in light the compositional complexity matrix, treatment with multiple enzymes enhance reduction formation compared single enzyme therapy. present study investigated effect three matrix-degrading...

Background Combating dental biofilm formation is the most effective means for prevention of caries, one widespread human diseases. Among chemical supplements to mechanical tooth cleaning procedures, non-bactericidal adjuncts that target mechanisms bacterial have gained increasing interest in recent years. Milk proteins, such as lactoferrin, been shown interfere with colonization saliva-coated surfaces. We here study effect bovine milk osteopontin (OPN), a highly phosphorylated whey...

The pH in bacterial biofilms on teeth is of central importance for dental caries, a disease with high worldwide prevalence. Nutrients and metabolites are not distributed evenly biofilms. A complex interplay sorption to reaction organic matter the biofilm reduces diffusion paths solutes creates steep gradients reactive molecules, including acids, across biofilm. Quantitative fluorescent microscopic methods, such as fluorescence life time imaging or ratiometry, can be employed visualize...

Bacterial biofilms have a complex and heterogeneous three-dimensional architecture that is characterized by chemically structurally distinct microenvironments. Confocal microscopy-based pH ratiometry fluorescence lectin-binding analysis (FLBA) are well-established methods to characterize developments the carbohydrate matrix of at microscale. Here, we developed combined analysis, pH-FLBA, concomitantly map biofilm distribution carbohydrates in bacterial while preserving microarchitecture. As...

The extracellular matrix is a poorly studied, yet important component of dental biofilms. Fluorescence lectin-binding analysis (FLBA) powerful tool to characterize glycoconjugates in the biofilm matrix. This study aimed systematically investigate ability 75 fluorescently labeled lectins visualize and quantify Lectin binding was screened on pooled supragingival samples collected from 76 subjects using confocal microscopy. FLBA then performed with 10 selected biofilms grown situ for 48 h...

While probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding effects on complex dental biofilms. We assessed two probiotics, Lactobacillus reuteri Streptococcus oligofermentans, continuous-cultured model containing mutans, rhamnosus Actinomyces naeslundii. Cariogenic biofilms were grown bovine enamel specimens daily challenged with L. or S. oligofermentans whole culture (LC/SC) cell-free supernatant (LS/SS) medium only...