A5007467831- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Bacillus and Francisella bacterial research
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Peripheral Neuropathies and Disorders
- Virus-based gene therapy research
- Toxin Mechanisms and Immunotoxins
- Poxvirus research and outbreaks
- Multiple Sclerosis Research Studies
- COVID-19 epidemiological studies
- Biosensors and Analytical Detection
- Biochemical Analysis and Sensing Techniques
- Animal Virus Infections Studies
- COVID-19 Digital Contact Tracing
- Hepatitis B Virus Studies
- RNA regulation and disease
- Piperaceae Chemical and Biological Studies
- Viral Infections and Immunology Research
- Infection Control and Ventilation
- Vector-Borne Animal Diseases
- Vaccine Coverage and Hesitancy
Kiel University
2018-2024
University of Lübeck
2020-2023
University Hospital Schleswig-Holstein
2020-2023
Universitäts Hautklinik Kiel
2023
Clinical Research Center Kiel
2023
National Institute of Standards and Technology
2007
Cytel (United States)
1995
Scripps Research Institute
1995
Our goal is to use peptide epitopes that are recognized by cytotoxic T lymphocytes (CTL) as immunogens for the development of prophylactic and therapeutic vaccines with chronic hepatitis B virus (HBV) infection being our first target.Because most CTL poor immunogens, we specifically modified them cova- lently attaching two additional components: a helper pep- tide epitope lipid molecules.Using murine in- fluenza NP 147-155 model system, found this construct be highly immunogenic, single...
The rapid detection of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is necessary in ongoing pandemic. Antigen-specific point-of-care tests (POCT) may be useful for this purpose. Here, such a POCT (SARS-CoV-2 NADAL® COVID-19 Ag) was compared to laboratory-developed triplex real-time polymerase chain reaction (RT-PCR) designed viral nucleoprotein gene and two control targets. This RT-PCR served as reference investigate sensitivity re-testing upper tract...
Abstract Background The humoral immune response after primary immunisation with a SARS-CoV-2 vector vaccine (AstraZeneca AZD1222, ChAdOx1 nCoV-19, Vaxzevria) followed by an mRNA boost (Pfizer/BioNTech, BNT162b2; Moderna, m-1273) was examined and compared the antibody homologous vaccination schemes (AZD1222/AZD1222 or BNT162b2/BNT162b2). Methods Sera from 59 vaccinees were tested for anti-SARS-CoV-2 immunoglobulin G (IgG) virus-neutralising antibodies (VNA) three IgG assays based on (parts...
Evolving SARS-CoV-2 variants and changing levels of pre-existing immunity require re-evaluation antigen-detecting rapid diagnostic test (Ag-RDT) performance. We investigated possible associations between Ag-RDT sensitivity various potential influencing factors, such as immunisation status viral variant, in symptomatic hospital employees.
Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) has developed substantial antigenic variability. As the majority of population now pre-existing immunity due to infection or vaccination, use experimentally generated animal immune sera can be valuable for measuring differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one nine SARS-CoV-2 Their were titrated against 16 variants, and resulting titers visualized using cartography....
Kinetics of neutralizing antibodies and immunoglobulin G (IgG) against the nucleo (N) or spike (S) proteins severe acute respiratory syndrome coronavirus type2 (SARS-CoV-2) were studied in patients up to 165 days after PCR diagnosis infection. Two immunoassays selected out eight IgG total antibody tests by comparing their specificities sensitivities. Sensitivities calculated with convalescent sera from 26 PCR-confirmed cases, which 76.9% had (>1:10). Stored collected during summer 2018 (N...
COVID-19 vaccines are highly effective in inducing protective immunity. While the serum antibody response to vaccination has been studied depth, our knowledge of underlying plasmablast and memory B cell (Bmem) responses is still incomplete. Here, we determined a naïve population contrasted it with single influenza primed cohort. In addition, analyzed against four endemic human coronaviruses (HCoVs).
The humoral immunity after SARS-CoV-2 infection or vaccination was examined. Convalescent sera with variants of concern (VOCs: B.1.1.7, n = 10; B.1.351, 1) and from 100 vaccinees (Pfizer/BioNTech, BNT162b2, 33; Moderna, mRNA-1273, 11; AstraZeneca, ChAdOx1 nCoV-19/AZD1222, 56) were tested for the presence immunoglobulin G (IgG) directed against viral spike (S)-protein, its receptor-binding domain (RBD), nucleoprotein (N) virus-neutralizing antibodies (VNA). For latter, surrogate assays (sVNT)...
Abstract The humoral immune response to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) vaccination in patients with chronic inflammatory disease (CID) declines more rapidly tumor necrosis factor‐α (TNF‐α) inhibition. Furthermore, the efficacy of current vaccines against Omicron variants concern (VOC) including BA.2 is limited. Alterations within cell populations, changes IgG affinity, and ability neutralize a pre‐VOC strain virus were investigated these at‐risk patients. Serum...
Evolving SARS-CoV-2 variants and changing levels of pre-existing immunity require re-evaluation rapid antigen test performance. In hospital employees in Berlin, Germany, from late 2020 to early 2022, we found decreasing sensitivity over time, 80% 67%, despite a 5·6 fold increase median viral RNA concentrations symptomatic their infection course. These changes were observed parallel with sharp the percentage who at least doubly vaccinated or recovered infection. Based on statistical analyses...
Abstract SARS-CoV-2 has developed substantial antigenic variability. As the majority of population now pre-existing immunity due to infection or vaccination, use experimentally generated animal immune sera can be valuable for measuring differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one eight Their were titrated against 14 variants and resulting titers visualized using cartography. The map shows a condensed cluster containing all...
Abstract The reliable detection of immunoglobulin G (IgG) or total antibodies directed against the novel severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is important for clinical diagnostics and epidemiological studies. Here, we compare diagnostic accuracy six commercially available SARS-CoV-2 IgG (Abbott IgG; Diasorin Liaison ® S1/2 Epitope EDI™ Novel Coronavirus COVID-19 ELISA Kit; Euroimmun Anti-SARS-CoV-2 (IgG); Mikrogen recom Well IgG) antibody assays (Roche Elecsys...
The occurrence of immune-evasive SARS-CoV-2 strains emphasizes the importance to search for broad-acting antiviral compounds. Our previous in vitro study showed that Pelargonium sidoides DC. root extract EPs® 7630 has combined and immunomodulatory properties SARS-CoV-2-infected human lung cells. Here we assessed vivo effects hamsters, investigated its functionally relevant constituents context phenotypically distinct variants. We show reduced viral load early course infection displayed...
To analyze anti-SARS-CoV-2-S1-IgG levels, avidity, Omicron BA.2 variant neutralizing capacity, and SARS-CoV-2-specific T cells in anti-CD20-treated patients with multiple sclerosis (aCD20pwMS) after two, three, or four COVID-19 vaccinations.Frequencies of aCD20pwMS detectable SARS-CoV-2-S1-IgG increased moderately between two (31/61 (51%)), three (31/57 (54%)), (17/26 (65%)) vaccinations. However, among SARS-CoV-2-S1-IgG, frequencies high avidity (6/31 (19%) vs 11/17 antibodies (0/10 (0%)...
The recurrent emerging of novel viral variants concern (VOCs) with evasion preexisting antibody immunity upholds severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) case numbers and maintains a persistent demand for updated therapies. We selected the patient-derived CV38-142 based on its potency breadth against VOCs Alpha, Beta, Gamma, Delta preclinical development into therapeutic. showed in vivo efficacy Syrian hamster VOC infection model after post-exposure therapeutic...
Oxford Nanopore Technologies (ONT) allows direct sequencing of ribonucleic acids (RNA) and, in addition, detection possible RNA modifications due to deviations from the expected ONT signal. The software available so far for this purpose can only detect a small number modifications. Alternatively, two samples be compared different We present Magnipore, novel tool search significant signal shifts between data similar or related species. Magnipore classifies them into mutations and potential...
Abstract Background The humoral immune response after primary immunisation with a SARS-CoV-2 vector vaccine (AstraZeneca AZD1222, ChAdOx1 nCoV-19, Vaxzevria) followed by an mRNA boost (Pfizer/BioNTech, BNT162b2; Moderna, m-1273) was examined and compared the antibody homologous vaccination schemes (AZD1222/AZD1222 or BNT162b2/BNT162b2). Methods Sera from 59 vaccinees were tested for anti-SARS-CoV-2 immunoglobulin G (IgG) virus-neutralising antibodies (VNA) four IgG assays, surrogate...
LetterDelta or Omicron BA.1/2-neutralizing antibody levels and T-cell reactivity after triple-vaccination infectionTo the editor,In Germany, SARS-CoV-2 infections in fall 2021 were caused by Delta variant of concern (VOC B.1.617.2), which was completely replaced VOC (BA.1, B.1.529.1/BA.2, B.1.529.2) winter. Meanwhile, BA.2 sublineage dominates, apparently having a selection advantage1.We studied kinetics anti-spike (S) protein IgG neutralizing antibodies (NA) as well release interferon-gamma...