A5030146759- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Respiratory and Cough-Related Research
- Piperaceae Chemical and Biological Studies
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Biochemical Analysis and Sensing Techniques
- Autophagy in Disease and Therapy
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Liver Disease Diagnosis and Treatment
- Respiratory viral infections research
- Virology and Viral Diseases
- Malaria Research and Control
- COVID-19 impact on air quality
- vaccines and immunoinformatics approaches
- Ion Channels and Receptors
- COVID-19 epidemiological studies
- Long-Term Effects of COVID-19
- Liver Disease and Transplantation
- Endoplasmic Reticulum Stress and Disease
- Evolution and Genetic Dynamics
Charité - Universitätsmedizin Berlin
2020-2024
Queen Mary University of London
2024
German Center for Infection Research
2020-2024
Washington State University
2024
University of Nairobi
2024
University of Edinburgh
2024
Freie Universität Berlin
2020-2023
Humboldt-Universität zu Berlin
2020-2023
National Institute of Allergy and Infectious Diseases
2018-2021
National Institutes of Health
2018-2021
Viruses manipulate cellular metabolism and macromolecule recycling processes like autophagy. Dysregulated might lead to excessive inflammatory autoimmune responses as observed in severe long COVID-19 patients. Here we show that SARS-CoV-2 modulates reduces Accordingly, compound-driven induction of autophagy limits propagation. In detail, SARS-CoV-2-infected cells accumulation key metabolites, activation inhibitors (AKT1, SKP2) reduction proteins responsible for initiation (AMPK, TSC2, ULK1),...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses an threat to public health and the world economy, especially because no approved specific drugs or vaccines are available. Pharmacological modulation of metabolism-dependent cellular pathways such as autophagy reduced propagation highly pathogenic Middle East (MERS)-CoV. Here we show that SARS-CoV-2 infection limits by interfering with multiple metabolic compound-driven interventions aimed at induction reduce in...
Abstract Infection with Zika virus (ZIKV) is commonly mild in humans but has been associated alarming negative health outcomes including Guillain-Barré syndrome adults and microcephaly fetuses. As such, developing a vaccine for ZIKV global public priority. Recombinant vesicular stomatitis (VSV) expressing the Ebola (EBOV) glycoprotein (GP) successfully used as platform past. In this study, two novel VSV-ZIKV vaccines were generated utilizing favorable immune targeting of existing VSV-EBOV...
Ebola virus (EBOV), isolate Makona, was the causative agent of West African epidemic devastating predominantly Guinea, Liberia and Sierra Leone from 2013-2016. While several experimental vaccine treatment approaches have been accelerated through human clinical trials, there is still no approved countermeasure available against this disease. Here, we report construction preclinical efficacy testing a novel recombinant modified vaccinia Ankara (MVA)-based expressing EBOV-Makona glycoprotein GP...
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) is endemic in dromedaries Africa, but camel-to-human transmission limited. Sustained 12-month sampling of a Kenya abattoir hub showed biphasic MERS-CoV incidence; peak detections occurred October 2022 and February 2023. Dromedary-exposed workers (7/48) had serologic signs previous exposure.
Treatment options for COVID-19 are currently limited. Drugs reducing both viral loads and SARS-CoV-2-induced inflammatory responses would be ideal candidates therapeutics. Previous in vitro clinical studies suggest that the proprietary Pelargonium sidoides DC. root extract EPs 7630 has antiviral immunomodulatory properties, limiting symptom severity disease duration of infections with several upper respiratory viruses. Here we assessed if affects SARS-CoV-2 propagation innate immune response...
Epidemiological data demonstrate that Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) Alpha and Delta are more transmissible, infectious, pathogenic than previous variants. Phenotypic properties VOC remain understudied. Here, we provide an extensive functional study replication cell entry phenotypes assisted by reverse genetics, mutational mapping spike in lentiviral pseudotypes, viral cellular gene expression studies, infectivity stability assays...
The common small animal disease models for Zika virus (ZIKV) are mice lacking the interferon responses, but infection of receptor α/β knock out (IFNAR-/-) is not uniformly lethal particularly in older animals. Here we sought to advance this model regard lethality future countermeasure efficacy testing against more recent ZIKV strains from Asian lineage, preferably American sublineage. We first infected IFNAR-/- subcutaneously with contemporary ZIKV-Paraiba strain resulting predominantly...
The occurrence of immune-evasive SARS-CoV-2 strains emphasizes the importance to search for broad-acting antiviral compounds. Our previous in vitro study showed that Pelargonium sidoides DC. root extract EPs® 7630 has combined and immunomodulatory properties SARS-CoV-2-infected human lung cells. Here we assessed vivo effects hamsters, investigated its functionally relevant constituents context phenotypically distinct variants. We show reduced viral load early course infection displayed...
Intrinsic fitness costs are likely to have guided the selection of lineage-determining mutations during emergence variants SARS-CoV-2. Whereas changes in receptor affinity and antibody neutralization been thoroughly mapped for individual spike, their influence on intrinsic replicative remains understudied.We analyzed immunodominant spike epitope E484 that became temporarily fixed over pandemic. We engineered resulting immune escape E484K, -A, -Q recombinant characterized viral replication,...
Zika virus (ZIKV), a member of the Flaviviridae family, is an important human pathogen that has caused epidemics in Africa, Southeast Asia, and Americas. No licensed treatments for ZIKV disease are currently available. Favipiravir (T-705; 6-fluoro-3-hydroxy-2-pyrazinecarboxamide) ribavirin (1-(β-D-Ribofuranosyl)-1H-1,2,4-triazole-3-carboxamide) nucleoside analogs have exhibited antiviral activity against broad spectrum RNA viruses, including some flaviviruses. In this study, we strengthened...
ABSTRACT Epidemiological data demonstrate that SARS-CoV-2 variants of concern (VOC) B.1.1.7 and B.1.617.2 are more transmissible infections associated with a higher mortality than non-VOC virus infections. Phenotypic properties underlying their enhanced spread in the human population remain unknown. isolates displayed inferior or equivalent most cell lines primary cells compared to an ancestral B.1 SARS-CoV-2, were outcompeted by latter. Lower infectivity delayed entry kinetics viruses...