A5074494709- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Peptidase Inhibition and Analysis
- Piperaceae Chemical and Biological Studies
- Respiratory and Cough-Related Research
- Protein Degradation and Inhibitors
- Plant Virus Research Studies
- Bacillus and Francisella bacterial research
- Hip disorders and treatments
- COVID-19 epidemiological studies
- Medical Imaging and Pathology Studies
- Biochemical Analysis and Sensing Techniques
- Hepatitis B Virus Studies
- Ion Channels and Receptors
- Autophagy in Disease and Therapy
- Orthopaedic implants and arthroplasty
- Spine and Intervertebral Disc Pathology
- Endoplasmic Reticulum Stress and Disease
- Spinal Fractures and Fixation Techniques
- Neuroendocrine Tumor Research Advances
- vaccines and immunoinformatics approaches
- Pneumonia and Respiratory Infections
Freie Universität Berlin
2018-2024
Charité - Universitätsmedizin Berlin
2017-2024
Humboldt-Universität zu Berlin
2018-2024
Institute of Virology of the Slovak Academy of Sciences
2023
German Center for Infection Research
2020-2021
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2018
University of Bonn
1982-2017
Viruses manipulate cellular metabolism and macromolecule recycling processes like autophagy. Dysregulated might lead to excessive inflammatory autoimmune responses as observed in severe long COVID-19 patients. Here we show that SARS-CoV-2 modulates reduces Accordingly, compound-driven induction of autophagy limits propagation. In detail, SARS-CoV-2-infected cells accumulation key metabolites, activation inhibitors (AKT1, SKP2) reduction proteins responsible for initiation (AMPK, TSC2, ULK1),...
SummaryBackgroundThe COVID-19 agent, SARS-CoV-2, is conspecific with SARS-CoV, the causal agent of severe acute respiratory syndrome epidemic in 2002–03. Although viruses share a completely homologous repertoire proteins and use same cellular entry receptor, their transmission efficiencies pathogenetic traits differ. We aimed to compare interferon antagonism by SARS-CoV SARS-CoV-2.MethodsFor this functional study, we infected Vero E6 Calu-3 cells strains SARS-CoV-2. studied differences cell...
Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) causes a severe disease in humans. The MERS-CoV spike (S) glycoprotein mediates viral entry into target cells. For this, S engages the host cell protein dipeptidyl peptidase 4 (DPP4, CD26) and interface between DPP4 has been resolved on atomic level. Here, we asked whether naturally-occurring polymorphisms DPP4, that alter amino acid residues required for binding, influence cellular of MERS-CoV. By screening public databases,...
Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) has developed substantial antigenic variability. As the majority of population now pre-existing immunity due to infection or vaccination, use experimentally generated animal immune sera can be valuable for measuring differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one nine SARS-CoV-2 Their were titrated against 16 variants, and resulting titers visualized using cartography....
Significance Hepatitis delta virus (HDV) aggravates hepatitis B (HBV) infection of liver cells. Although the viruses are evolutionarily unrelated, HDV depends on HBV because it requires envelope protein for its transmission. is only described in humans, which has triggered diverse hypotheses regarding evolution and origins. Here we show that spiny rats ( Proechimys semispinosus ) carry a counterpart to surprisingly does not cause linked HBV. The rodent deltavirus finding alone, but also...
Treatment options for COVID-19 are currently limited. Drugs reducing both viral loads and SARS-CoV-2-induced inflammatory responses would be ideal candidates therapeutics. Previous in vitro clinical studies suggest that the proprietary Pelargonium sidoides DC. root extract EPs 7630 has antiviral immunomodulatory properties, limiting symptom severity disease duration of infections with several upper respiratory viruses. Here we assessed if affects SARS-CoV-2 propagation innate immune response...
Epidemiological data demonstrate that Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) Alpha and Delta are more transmissible, infectious, pathogenic than previous variants. Phenotypic properties VOC remain understudied. Here, we provide an extensive functional study replication cell entry phenotypes assisted by reverse genetics, mutational mapping spike in lentiviral pseudotypes, viral cellular gene expression studies, infectivity stability assays...
Abstract SARS-CoV-2 has developed substantial antigenic variability. As the majority of population now pre-existing immunity due to infection or vaccination, use experimentally generated animal immune sera can be valuable for measuring differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one eight Their were titrated against 14 variants and resulting titers visualized using cartography. The map shows a condensed cluster containing all...
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the and Africa. Virus-induced pneumonia humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after novel recombinant clade (lineage 5) caused large nosocomial outbreaks Saudi Arabia South Korea 2016. However, there has no functional assessment. Here we perform comprehensive vitro ex vivo comparison of viruses parental...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a high-priority pathogen in pandemic preparedness research. Reverse genetics systems are valuable tool to study viral replication and pathogenesis, design attenuated vaccines create defined assay for applications such as antiviral screening. Here we present novel reverse system MERS-CoV that involves maintenance of the full-length genome cDNA copy inserted bacterial artificial chromosome amenable manipulation by homologue...
The orthopaedic surgeon should be familiar with various supports and braces for the treatment of low back pain. Severe cases spinal instability always need a Hohmann overbridging brace, whereas milder form motion-segment is treated one elastic supports. In osteoporosis spine insufficiency lumbosacral junction Lindemann 2/3 semi-elastic brace prescribed.
ABSTRACT Epidemiological data demonstrate that SARS-CoV-2 variants of concern (VOC) B.1.1.7 and B.1.617.2 are more transmissible infections associated with a higher mortality than non-VOC virus infections. Phenotypic properties underlying their enhanced spread in the human population remain unknown. isolates displayed inferior or equivalent most cell lines primary cells compared to an ancestral B.1 SARS-CoV-2, were outcompeted by latter. Lower infectivity delayed entry kinetics viruses...
Inhibitors of bromodomain and extra-terminal proteins (iBETs), including JQ-1, have been suggested as potential prophylactics against SARS-CoV-2 infection. However, molecular mechanisms underlying JQ-1-mediated antiviral activity its susceptibility to viral subversion remain incompletely understood. Pretreatment cells with iBETs inhibited infection by variants SARS-CoV, but not MERS-CoV. The manifested itself reduced reporter expression recombinant viruses, RNA quantities infectious titers...
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) can cause severe pneumonia in humans. The virus is enzootic dromedary camels across the and Africa. It acquired through animal contact undergoes limited onward transmission particularly hospitals. Because of this initial potential for human-to-human transmission, we monitor phenotypic changes related to its pandemic potential. Potential have been suspected since year 2015, when a novel recombinant clade (MERS-CoV lineage 5)...
ABSTRACT Inhibitors of bromodomain and extra-terminal proteins (iBETs), including JQ-1, have been suggested as potential therapeutics against SARS-CoV-2 infection. However, molecular mechanisms underlying JQ-1-induced antiviral activity its susceptibility to viral antagonism remain incompletely understood. iBET treatment transiently inhibited infection by variants SARS-CoV, but not MERS-CoV. Our functional assays confirmed JQ-1-mediated downregulation ACE2 expression multi-omics analysis...