A5057269323- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Antimicrobial agents and applications
- Bacillus and Francisella bacterial research
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Orthopedic Infections and Treatments
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
Freie Universität Berlin
2022-2025
Abstract Vaccines play a critical role in combating the COVID-19 pandemic. Future control of pandemic requires improved vaccines with high efficacy against newly emerging SARS-CoV-2 variants and ability to reduce virus transmission. Here we compare immune responses preclinical mRNA vaccine BNT162b2, adenovirus-vectored spike Ad2-spike live-attenuated candidate sCPD9 Syrian hamsters, using both homogeneous heterologous vaccination regimens. Comparative was assessed by employing readouts from...
Abstract The development of effective SARS-CoV-2 vaccines has been essential to control COVID-19, but significant challenges remain. One problem is intramuscular administration, which does not induce robust mucosal immune responses in the upper airways—the primary site infection and virus shedding. Here we compare efficacy a mucosal, replication-competent yet fully attenuated vaccine, sCPD9-ΔFCS, monovalent mRNA vaccine BNT162b2 preventing transmission variants B.1 Omicron BA.5 two...
Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) has developed substantial antigenic variability. As the majority of population now pre-existing immunity due to infection or vaccination, use experimentally generated animal immune sera can be valuable for measuring differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one nine SARS-CoV-2 Their were titrated against 16 variants, and resulting titers visualized using cartography....
The open reading frame 3a (ORF3a) is a protein important to the pathogenicity of SARS-CoV-2. cytoplasmic domain ORF3a has three canonical tyrosine-based sorting signals (160YNSV163, 211YYQL213, and 233YNKI236), previous study indicated that mutation 160YNSV163 motif abrogated plasma membrane expression inhibited ORF3a-induced apoptosis. Here, we have systematically removed all motifs assessed importance each or combination in trafficking cell surface. Our results indicate alone was...
Live attenuated vaccines (LAVs) administered via the mucosal route may offer better control of COVID-19 pandemic than non-replicating injected intramuscularly. Conceptionally, LAVs have several advantages, including presentation entire antigenic repertoire virus, and induction strong immunity. Thus, immunity induced by LAV could superior protection against future surges cases caused emerging SARS-CoV-2 variants. However, carry risk unintentional transmission. To address this issue, we...
Abstract Antiviral innate immunity represents the first defense against invading viruses and is key to control viral infections, including SARS-CoV-2. Body temperature an omnipresent variable but was neglected when addressing host mechanisms susceptibility SARS-CoV-2 infection. Here, we show that increasing in a 1.5°C window, between 36.5 38°C, strongly increases expression of genes two branches antiviral immunity, nitric oxide production type I interferon response. We alternative splicing...
Abstract SARS-CoV-2 has developed substantial antigenic variability. As the majority of population now pre-existing immunity due to infection or vaccination, use experimentally generated animal immune sera can be valuable for measuring differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one eight Their were titrated against 14 variants and resulting titers visualized using cartography. The map shows a condensed cluster containing all...
Mucins are the key component of defensive mucus barrier. They extended fibers very high molecular weight with diverse biological functions depending strongly on their specific structural parameters. Here, we present a mucin-inspired nanostructure, produced via synthetic methodology to prepare methacrylate-based dendronized polysulfates (MIP-1) multi gram-scale (MW=450 kDa) and thiol end-functionalized polymer (MIP) RAFT polymerization. Cryo-electron tomography (Cryo-ET) analysis MIP-1...
Mucus is the first defense barrier against viruses in human immune system. Inspired by mucus structure, we designed a highly sulfated hydrogel to bind and prevent infection of underlying cells. The was formed gelation cellulose nanofiber (SCNF) with Ca
Abstract Vaccines are a cornerstone in COVID-19 pandemic management. Here, we compare immune responses to and preclinical efficacy of the mRNA vaccine BNT162b2, an adenovirus-vectored spike vaccine, live-attenuated-virus candidate sCPD9 after single double vaccination Syrian hamsters. All regimens containing showed superior efficacy. The robust immunity elicited by was evident wide range parameters challenge with heterologous SARS-CoV-2 including rapid viral clearance, reduced tissue damage,...
T492I, a mutation encountered in SARS-CoV-2 nonstructural protein 4 (NSP4), enhances viral replication and alters cleavage, causing potential evolutionary impacts. Through comprehensive comparative analyses based on evolve-and-resequence experiments of wild-type Delta strains with or without we demonstrate that NSP4 T492I not only increases the rate, but also accelerates emergence many mutations characteristic for Omicron variants. Accordingly, populations evolved from ancestors show...
Abstract Mucine sind eine Schlüsselkomponente der defensiven Schleimbarriere. Es handelt sich um ausgedehnte Fasern mit sehr hohem Molekulargewicht, deren vielfältige biologische Funktionen stark von ihren spezifischen Strukturparametern abhängen. Hier stellen wir Mucin‐inspirierte Nanostruktur basierend auf dendronisierten Polysulfaten Methacrylatbasis ( MIP‐1 ) Molekulargewicht (MW=450 kDa) vor. Das Thiol‐End‐funktionalisierte Mucin‐inspiriertem Polymer MIP wurde durch RAFT‐Polymerisation...