A5025349666- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- interferon and immune responses
- COVID-19 diagnosis using AI
- Machine Learning in Bioinformatics
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Extracellular vesicles in disease
- Bioinformatics and Genomic Networks
- Monoclonal and Polyclonal Antibodies Research
- Mosquito-borne diseases and control
- Cell Image Analysis Techniques
- Galectins and Cancer Biology
- Protein Degradation and Inhibitors
- Autoimmune and Inflammatory Disorders Research
- Animal Disease Management and Epidemiology
- HIV/AIDS drug development and treatment
- Influenza Virus Research Studies
Institute of Virology of the Slovak Academy of Sciences
2020-2025
Charité - Universitätsmedizin Berlin
2020-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020-2024
Freie Universität Berlin
2020-2024
Humboldt-Universität zu Berlin
2020-2024
Liverpool School of Tropical Medicine
2024
Ludwig-Maximilians-Universität München
2021
Innsbruck Medical University
2021
In COVID-19, immune responses are key in determining disease severity. However, cellular mechanisms at the onset of inflammatory lung injury SARS-CoV-2 infection, particularly involving endothelial cells, remain ill-defined. Using Syrian hamsters as a model for moderate we conduct detailed longitudinal analysis systemic and pulmonary responses, corroborate it with datasets from COVID-19 patients. Monocyte-derived macrophages lungs exert earliest strongest transcriptional response to...
Vaccine-associated enhanced respiratory disease (VAERD) is a severe complication for some infections. To investigate the potential VAERD induction in coronavirus 2019 (COVID-19), we evaluate two vaccine leads utilizing hamster infection model: T helper type 1 (TH1)-biased measles vaccine-derived candidate and TH2-biased alum-adjuvanted, non-stabilized spike protein. The virus (MeV)-derived protects animals, but protein lead induces VAERD, which can be alleviated by dexamethasone treatment....
For coronavirus disease 2019 (COVID-19), effective and well-understood treatment options are still scarce. Since vaccine efficacy is challenged by novel variants, short-lasting immunity, hesitancy, understanding optimizing therapeutic remains essential. We aimed at better the effects of two standard-of-care drugs, dexamethasone anti-severe acute respiratory syndrome 2 (SARS-CoV-2) antibodies, on infection host responses. By using COVID-19 hamster models, pulmonary immune responses were...
The accessory protease transmembrane serine 2 (TMPRSS2) enhances severe acute respiratory syndrome coronavirus (SARS-CoV-2) uptake into ACE2-expressing cells, although how increased entry impacts downstream viral and host processes remains unclear. To investigate this in more detail, we performed infection assays engineered cells promoting ACE2-mediated with without TMPRSS2 coexpression. Electron microscopy inhibitor experiments indicated TMPRSS2-mediated cell was associated virion...
Infection by (re-)emerging RNA arboviruses including Chikungunya virus (CHIKV) and Mayaro primarily cause acute febrile disease transient polyarthralgia. However, in a significant subset of infected individuals, debilitating arthralgia persists for weeks over months up to years. The underlying immunopathogenesis chronification upon primary RNA-viral infection remains unclear. Here, we analysed cell-intrinsic responses ex vivo arthritogenic alphaviral human synovial fibroblasts isolated from...
Translating findings from animal models to human disease is essential for dissecting mechanisms, developing and testing precise therapeutic strategies. The coronavirus 2019 (COVID-19) pandemic has highlighted this need, particularly showing severity-dependent immune responses. Single-cell transcriptomics (scRNAseq) well poised reveal similarities differences between species at the molecular cellular level with unprecedented resolution. However, computational methods enabling detailed...
In COVID-19, the immune response largely determines disease severity and is key to therapeutic strategies. Cellular mechanisms contributing inflammatory lung injury tissue repair in SARS-CoV-2 infection, particularly endothelial cell involvement, remain ill-defined. We performed detailed spatiotemporal analyses of cellular molecular processes infected Syrian hamsters. Comparison hamster single-cell sequencing proteomics with data sets from COVID-19 patients demonstrated inter-species...
Abstract Glycoprotein 90K, encoded by the interferon-stimulated gene LGALS3BP , displays broad antiviral activity. It reduces HIV-1 infectivity interfering with Env maturation and virion incorporation, increases survival of Influenza A virus-infected mice via innate immune signaling. Its potential in SARS-CoV-2 infection remains largely unknown. Here, we analyzed expression 90K/ 44 hospitalized COVID-19 patients at multiple levels. We quantified 90K protein concentrations serum PBMCs as well...
Abstract Key issues for research of COVID-19 pathogenesis are the lack biopsies from patients and samples at onset infection. To overcome these hurdles, hamsters were shown to be useful models studying this disease. Here, we further leveraged model molecularly survey disease progression time-resolved single-cell RNA-sequencing data collected healthy SARS-CoV-2-infected Syrian Roborovski hamster lungs. We compared our human studies, including BALF, nasal swab, post-mortem lung tissue,...
Abstract In recent years, (re-)emerging arboviruses including Chikungunya virus (CHIKV) and Mayaro (MAYV) have caused growing concern due to expansion of insect vector ranges. No protective vaccine or specific antiviral strategies are currently available. Long-term morbidity after CHIKV infection includes debilitating chronic joint pain, which has associated health economic impact. Here, we analyzed the early cell-intrinsic response MAYV in primary human synovial fibroblasts. This...
In coronavirus disease 2019 (COVID-19) research, hamster species are particularly valuable to study host-pathogen interactions as they develop moderate (Mesocricetus auratus) or severe (Phodopus roborovskii) courses following infection. To identify transcriptional similarities between hamsters and humans in the early phase of SARS-CoV-2 infection, we analyzed blood leukocyte single-cell RNA-sequencing datasets from together with publicly available COVID-19 patients. Overall, both...
MPOX virus (MPXV), formerly known as monkeypox virus, led to a rapidly evolving pandemic starting May 2022, with over 90,000 cases reported beyond the African continent. This outbreak was driven by MPXV variant Clade IIb. In addition, I viruses circulating in Democratic Republic of Congo (DRC) are drawing increased attention constantly rise and Ib, first identified 2023, is now co-circulating Ia seems exhibit enhanced human-to-human transmissibility. While most infected individuals display...
Abstract Cell-intrinsic responses mounted in vivo PBMCs during mild and severe COVID-19 differ quantitatively qualitatively. Whether they are triggered by signals emitted productively infected cells of the respiratory tract or are, at least partially, resulting from physical interaction with virus particles, remains unclear. Here, we analyzed susceptibility expression profiles healthy donors upon ex exposure to SARS-CoV SARS-CoV-2. In line absence detectable ACE2 receptor expression, human...
ABSTRACT Shock-and-kill is one of the most advanced, yet unrealized, concepts towards establishment HIV-1 cure. Treatment with latency-reversing agents (LRAs), including histone deacetylase inhibitors (HDACis) exerting chromatin remodelling and gene expression reprogramming, combined anti-retroviral therapy reactivates transcription in vitro , ex vivo . However, HDACi treatment fails to significantly reduce size viral reservoir people living (PLHIV). Here, by combining scRNA-seq functional...
ABSTRACT Since December 2019, the novel human coronavirus SARS-CoV-2 has spread globally, causing millions of deaths. Unprecedented efforts have enabled development and authorization a range vaccines, which reduce transmission rates confer protection against associated disease COVID-19. These vaccines are conceptually diverse, including e.g. classical adjuvanted whole-inactivated virus, viral vectors, mRNA vaccines. We analysed two prototypic model strongly T H 1-biased measles...
The ongoing corona virus disease 2019 (COVID-19) pandemic has led to an urgent demand for appropriate models depicting host-pathogen interactions and severity-dependent immune responses. Amongst various animal models, hamster species are particularly valuable as they permissive develop a moderate (Mesocricetus auratus) or severe (Phodopus roborovskii) course following infection. Here, we use single-cell ribonucleic acid sequencing of white blood cells dissect cell-specific changes in courses...
Inhibitors of bromodomain and extra-terminal proteins (iBETs), including JQ-1, have been suggested as potential prophylactics against SARS-CoV-2 infection. However, molecular mechanisms underlying JQ-1-mediated antiviral activity its susceptibility to viral subversion remain incompletely understood. Pretreatment cells with iBETs inhibited infection by variants SARS-CoV, but not MERS-CoV. The manifested itself reduced reporter expression recombinant viruses, RNA quantities infectious titers...
In the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic hamster species have been particularly valuable to study host-pathogen interactions as they are permissive develop a moderate (<i>Mesocricetus auratus</i>) or (<i>Phodopus roborovskii</i>) disease course following infection. Here we use single-cell ribonucleic acid sequencing of fresh whole blood, lysed erythrocytes, dissect cell-specific changes transcriptome in early stages and corona virus 2019 (COVID-19)...
Abstract Glycoprotein 90K, encoded by the interferon-stimulated gene LGALS3BP , displays broad antiviral activity. It reduces HIV-1 infectivity interfering with Env maturation and virion incorporation, increases survival of Influenza A virus-infected mice via innate immune signaling. Here, we analyzed expression 90K/ in 44 hospitalized COVID-19 patients. 90K protein serum levels were significantly elevated patients compared to uninfected sex- age-matched controls. Furthermore,...