Florence Pache
A5029030705- Multiple Sclerosis Research Studies
- Peripheral Neuropathies and Disorders
- Systemic Lupus Erythematosus Research
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Ocular Diseases and Behçet’s Syndrome
- CNS Lymphoma Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Polyomavirus and related diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Streptococcal Infections and Treatments
- Advanced Neuroimaging Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Pneumonia and Respiratory Infections
- Autoimmune Neurological Disorders and Treatments
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cerebral Venous Sinus Thrombosis
- Muscle and Compartmental Disorders
- Photoacoustic and Ultrasonic Imaging
- Glaucoma and retinal disorders
- T-cell and B-cell Immunology
- Metabolism and Genetic Disorders
Charité - Universitätsmedizin Berlin
2014-2024
Humboldt-Universität zu Berlin
2016-2024
Freie Universität Berlin
2018-2024
Heidelberg University
2016-2024
Euroimmun Medizinische Labordiagnostika (Germany)
2024
University Hospital of Basel
2019
Max Delbrück Center
2015-2018
St. Josef-Hospital
2018
Multiple Sclerosis Center Of Northeastern New York
2016
German Rheumatism Research Centre
2016
A subset of patients with neuromyelitis optica spectrum disorders (NMOSD) has been shown to be seropositive for myelin oligodendrocyte glycoprotein antibodies (MOG-IgG). To describe the epidemiological, clinical, radiological, cerebrospinal fluid (CSF), and electrophysiological features a large cohort MOG-IgG-positive optic neuritis (ON) and/or myelitis (n = 50) as well attack long-term treatment outcomes. Retrospective multicenter study. The sex ratio was 1:2.8 (m:f). Median age at onset 31...
Antibodies to myelin oligodendrocyte glycoprotein (MOG-IgG) have been suggested play a role in subset of patients with neuromyelitis optica and related disorders.To assess (i) the frequency MOG-IgG large predominantly Caucasian cohort optic neuritis (ON) and/or myelitis; (ii) among AQP4-IgG-positive vice versa; (iii) origin cerebrospinal fluid (CSF); (iv) presence at disease onset; (v) influence activity treatment status on titers.614 serum samples from ON myelitis controls, including 92...
See Zekeridou and Lennon (doi: 10.1093/aww213 ) for a scientific commentary on this article . Anti- N -methyl-D-aspartate receptor (NMDAR) encephalitis is recently discovered autoimmune syndrome associated with psychosis, dyskinesias, seizures. Little known about the cerebrospinal fluid autoantibody repertoire. Antibodies against NR1 subunit of NMDAR are thought to be pathogenic; however, direct proof lacking as previous experiments could not distinguish contribution further anti-neuronal...
Abstract Streptococcus pneumoniae is a leading cause of pneumonia, meningitis, and sepsis. Pneumococci can be divided into >90 serotypes that show differences in the pathogenicity invasiveness. We tested hypotheses innate immune inflammasome pathway involved fighting pneumococcal pneumonia some invasive types are not recognized by this pathway. human murine mononuclear cells responded to S. expressing hemolytic pneumolysin producing IL-1β. This IL-1β production depended on NOD-like...
Myelin oligodendrocyte glycoprotein antibodies (MOG-IgG) are present in a subset of aquaporin-4 (AQP4)-IgG-negative patients with optic neuritis (ON) and/or myelitis. Little is known so far about brainstem involvement MOG-IgG-positive patients. To investigate the frequency, clinical and paraclinical features, course, outcome, prognostic implications ON Retrospective case study. Among 50 myelitis, 15 (30 %) history encephalitis were identified. All negative for AQP4-IgG. Symptoms included...
Antibodies against myelin oligodendrocyte glycoprotein (MOG-IgG) have been reported in patients with aquaporin-4 antibody (AQP4-IgG)-negative neuromyelitis optica spectrum disorders (NMOSD). The objective of this study was to describe optic neuritis (ON)-induced neuro-axonal damage the retina MOG-IgG-positive comparison AQP4-IgG-positive NMOSD patients.Afferent visual system following ON bilaterally assessed 16 a history and compared that patients. In addition, healthy controls matched for...
To analyze whether 1 of the 2 apheresis techniques, therapeutic plasma exchange (PE) or immunoadsorption (IA), is superior in treating neuromyelitis optica spectrum disorder (NMOSD) attacks and to identify predictive factors for complete remission (CR).This retrospective cohort study was based on registry German Neuromyelitis Optica Study Group, a nationwide network established 2008. It recruited patients with diagnosed according 2006 Wingerchuk criteria aquaporin-4...
Abstract Background Comprehensive data on the cerebrospinal fluid (CSF) profile in patients with COVID-19 and neurological involvement from large-scale multicenter studies are missing so far. Objective To analyze systematically CSF COVID-19. Methods Retrospective analysis of 150 lumbar punctures 127 PCR-proven symptoms seen at 17 European university centers Results The most frequent pathological finding was blood-CSF barrier (BCB) dysfunction (median QAlb 11.4 [6.72–50.8]), which present...
Abstract Different NOD-like receptors, including NLRP1, NLRP3, and NLRC4, as well the recently identified HIN-200 protein, AIM2, form multiprotein complexes called inflammasomes, which mediate caspase-1–dependent processing of pro-IL-1β. Listeria monocytogenes is an intracellular pathogen that actively phagocytosed by monocytes/macrophages subsequently escapes from phagosome into host cell cytosol, depending on its pore-forming toxin listeriolysin O (LLO). In this study, we demonstrate human...
To analyse predictors for relapses and number of attacks under different immunotherapies in patients with neuromyelitis optica spectrum disorder (NMOSD).This is a retrospective cohort study conducted neurology departments at 21 regional university hospitals Germany. Eligible participants were aquaporin-4-antibody-positive or aquaporin-4-antibody-negative NMOSD. Main outcome measures HRs from Cox proportional hazard regression models adjusted centre effects, important prognostic factors...
To investigate depression frequency, severity, current treatment, and interactions with somatic symptoms among patients neuromyelitis optica spectrum disorder (NMOSD).In this dual-center observational study, we included 71 diagnosed NMOSD according to the International Panel for NMO Diagnosis 2015 criteria. The Beck Depression Inventory (BDI) was classified into severe, moderate, or minimal/no depressive state category. We used Fatigue Severity Scale evaluate fatigue. Scores from Brief Pain...
The functional dynamics and cellular sources of oxidative stress are central to understanding MS pathogenesis but remain elusive, due the lack appropriate detection methods. Here we employ NAD(P)H fluorescence lifetime imaging detect NADPH oxidases (NOX enzymes) in vivo identify inflammatory monocytes, activated microglia, astrocytes expressing NOX1 as major nervous system mice affected by experimental autoimmune encephalomyelitis (EAE). This directly affects neuronal function vivo,...
Background: Gender and age at onset are important epidemiological factors influencing prevalence, clinical presentation, treatment response in autoimmune diseases. Objective: To evaluate the impact of female sex fertile on aquaporin-4-antibody (AQP4-ab) status, attack localization, to patients with neuromyelitis optica (NMO) its spectrum disorders (neuromyelitis disorder (NMOSD)). Methods: Female-to-male ratios, diagnosis last visit (NMO vs NMOSD), treatment, outcome were compared according...
Background: In contrast to multiple sclerosis (MS), lesions in neuromyelitis optica (NMO) frequently contain neutrophils. However, the phenotypic profile of neutrophils these two distinct pathologies remains unknown. Objective: Our aim is better understand potential contribution NMO and MS pathology. Methods: We performed first functional analysis blood MS, including evaluation neutrophil immune response (fMLP receptor, TLR2), chemotaxis migration (CXCR1, CD62L, CD43), regulation complement...
To assess volumes and microstructural integrity of deep gray matter structures in a homogeneous cohort patients with neuromyelitis optica spectrum disorder (NMOSD).This was cross-sectional study including 36 aquaporin-4 antibody-positive (AQP4 Ab-positive) Caucasian NMOSD healthy controls matched for age, sex, education. Volumetry (DGM; thalamus, caudate, putamen, globus pallidus, hippocampus, amygdala, nucleus accumbens) performed using 2 independent automated methods. Microstructural...
To characterize paramagnetic MRI phase signal abnormalities in neuromyelitis optica spectrum disorder (NMOSD) vs multiple sclerosis (MS) lesions a cross-sectional study.Ten patients with NMOSD and 10 relapsing-remitting MS underwent 7-tesla brain including supratentorial T2*-weighted imaging susceptibility weighted imaging. Next, we analyzed intra- perilesional changes on filtered magnetic resonance images.We frequently observed rim-like (75 of 232 lesions, 32%) or nodular (32 14%) but only...
<h3>Objective:</h3> To assess in an observational study whether serum peptide antibody reactivities may distinguish aquaporin-4 (AQP4) (Ab)–positive and -negative neuromyelitis optica spectrum disorders (NMOSD) relapsing-remitting multiple sclerosis (RRMS). <h3>Methods:</h3> We screened 8,700 peptides that included human viral antigens of potential relevance for inflammatory demyelinating diseases random with pooled sera from different patient groups healthy controls to set up a customized...
<h3>Objectives</h3> To evaluate intrathecal immunoglobulin M (IgM) production, as compared to previously established risk factors, factor for conversion from clinically isolated syndrome (CIS) multiple sclerosis (MS) and explore the association of IgM production with onset age radiologic CSF findings in CIS/early MS. <h3>Methods</h3> Comprehensive data, including oligoclonal G (IgG) bands (OCB) calculated IgG were collected a prospective study 150 patients MS regular clinical MRI...
To investigate if patients with neuromyelitis optica spectrum disorder (NMOSD) develop subclinical visual pathway impairment independent of acute attacks.A total 548 longitudinally assessed full-field evoked potentials (VEP) 167 NMOSD from 16 centers were retrospectively evaluated for changes P100 latencies and P100-N140 amplitudes. Rates change in (RCL) amplitudes (RCA) over time analyzed each individual eye using linear regression compared generalized estimating equation models.The rates...
Severe sepsis and septic shock are leading causes of morbidity mortality worldwide. Infection-associated inflammation promotes the development progression adverse outcomes in sepsis. The effects heterodimeric IL-27 (p28/EBI3) have been implicated natural course sepsis, whereas molecular mechanisms underlying regulation gene expression release poorly understood. We studied events regulating p28 subunit endotoxic polymicrobial following cecal ligation puncture. Neutralizing Abs to IL-27(p28)...