A5017541498- Advanced Proteomics Techniques and Applications
- Chronic Kidney Disease and Diabetes
- Acute Kidney Injury Research
- Endoplasmic Reticulum Stress and Disease
- Renal and related cancers
- Mass Spectrometry Techniques and Applications
- Dialysis and Renal Disease Management
- Fungal and yeast genetics research
- Metabolomics and Mass Spectrometry Studies
- Renal Diseases and Glomerulopathies
- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Renal and Vascular Pathologies
- Bipolar Disorder and Treatment
- Bioinformatics and Genomic Networks
- Biosensors and Analytical Detection
- Amino Acid Enzymes and Metabolism
- Retinoids in leukemia and cellular processes
- Advanced Biosensing Techniques and Applications
- Skin and Cellular Biology Research
- Extracellular vesicles in disease
- Toxin Mechanisms and Immunotoxins
- Muscle metabolism and nutrition
- Metabolism, Diabetes, and Cancer
- Signaling Pathways in Disease
University of Göttingen
2015-2024
Universitätsmedizin Göttingen
2012-2024
Center for Rheumatology
2012-2022
Medizinische Hochschule Brandenburg Theodor Fontane
2019
Alexandria University
2018
RELX Group (United States)
2017
Heidelberg University
2016
Mosaiques Diagnostics and Therapeutics (Germany)
2010
Deutsche Forschungsgemeinschaft
2009
Max Planck Institute of Experimental Medicine
2009
Recommendations about structuring proteomic biomarker studies should increase the probability that such markers will be clinically useful.
Abstract Purpose : Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study the development well‐characterized “real life” sample that can be used reference standard in urine clinical studies. Experimental design We report on generation male and female samples are extensively characterized by different platforms methods (CE‐MS, LC‐MS, LC‐MS/MS, 1‐D gel analysis combination with nano‐LC MS/MS (using LTQ‐FT ultra), 2‐DE‐MS) their proteome peptidome. In...
In response to changes in the environment, yeast cells coordinate intracellular activities optimize survival and proliferation. The transductions of diverse extracellular stimuli are exerted through multiple mitogen-activated protein kinase (MAPK) cascades. high osmolarity glycerol (HOG) MAPK pathway is activated by increased environmental results a rise cellular concentration adapt osmotic pressure. We studied importance short time regulation glycolysis under hyperosmotic stress for...
Identification of markers for prediction the clinical course diabetic nephropathy remains a major challenge in disease management. We established proteomics approach identification nephropathy-related biomarkers urine.We used SELDI-TOF mass spectrometry and SAX2 protein arrays to compare profiles from urine 4 defined patient groups. Samples patients with type 2 diabetes (DM; n = 45) without microalbuminuria (DM-WNP), DM macro- or (DM-NP; 38), proteinuria due nondiabetic renal (n 34), healthy...
Intracellular pathogens including the apicomplexan and opportunistic parasite Toxoplasma gondii profoundly modify their host cells in order to establish infection. We have shown previously that intracellular T. inhibit up-regulation of regulatory effector functions murine macrophages (MΦ) stimulated with interferon (IFN)-γ, which is cytokine crucial for controlling parasites' replication. Using genome-wide transcriptome analysis we show herein infection leads global unresponsiveness IFN-γ....
Epithelial cells of the thick ascending limb Henle's loop (TALH cells) play a major role in urinary concentrating mechanism. They are normally exposed to variable and often very high osmotic stress, which is particularly due sodium chloride reabsorption low water permeability luminal membrane. It already established that elevation activity aldose reductase hence an increase intracellular sorbitol indispensable for adaptation stability TALH cells. To identify new molecular factors potentially...
The protein pattern of healthy human eccrine sweat was investigated and 10 major proteins were detected from which apolipoprotein D, lipophilin B, cathepsin D (CatD) identified for the first time in sweat. We focused our studies on function aspartate protease CatD In vitro digestion experiments using a specific fluorescent substrate showed that is enzymatically active To identify potential substrates LL-37 DCD-1L, two antimicrobial peptides present sweat, digested with purified CatD. not...
Abstract Background In multiple sclerosis relapses refractory to intravenous corticosteroid therapy, plasma exchange is recommended. Immunoadsorption (IA) regarded as an alternative but its efficacy and putative mechanism of action still needs be established. Methods We prospectively treated 11 patients with who had optical neuritis fulfilled the indications for apheresis therapy (Trial registration DE/CA25/00007080-00). total, five IA treatments were performed using tryptophan-IA. Clinical...
Renal fibrosis is characterized by excessive accumulation of extracellular matrix (ECM), which compromises organ function replacing normal tissue. The molecular mechanisms leading to renal are not fully understood. Here we demonstrated that TGFß1, AgII or PDGF stimulation cells resulted in ER-stress followed activation the protective unfolded protein response pathway and high secretory level disulfide isomerase ERP57. TGFß1 impairment ER-function could be reversed BMP7 treatment suggesting a...
<h3>Objective:</h3> To assess in an observational study whether serum peptide antibody reactivities may distinguish aquaporin-4 (AQP4) (Ab)–positive and -negative neuromyelitis optica spectrum disorders (NMOSD) relapsing-remitting multiple sclerosis (RRMS). <h3>Methods:</h3> We screened 8,700 peptides that included human viral antigens of potential relevance for inflammatory demyelinating diseases random with pooled sera from different patient groups healthy controls to set up a customized...
Our laboratory has previously demonstrated that Sirt1endo-/- mice show endothelial dysfunction and exaggerated renal fibrosis, whereas with silenced transforming growth factor beta (TGF-β) signaling are resistant to fibrogenic signals. Considering the fact only difference between these mutant is confined vascular endothelium, this indicates secreted substances contribute contrasting responses.We performed an unbiased proteomic analysis of secretome microvascular cells (RMVECs) isolated from...
Progressive fibrosis and maladaptive organ repair result in significant morbidity millions of premature deaths annually. Senescent cells accumulate with aging after injury are implicated fibrosis, but the mechanisms by which senescence influences poorly understood. Using 2 murine models repair, we show that obstructive generated senescent epithelia, persisted resolution original injury, promoted ongoing impeded adaptive repair. Depletion ABT-263 reduced reversed ureteric obstruction renal...
The search for novel molecular markers of tumor invasion is vital if strategies are to become more effective in the diagnostic and prognostic management transitional cell carcinoma bladder. Up 50% tumors detected at stage 1 (pT1) progress a higher grade even after endoscopic surgical resection, there currently no protein this aggressive, invasive phenotype. We have combined SELDI-TOF-MS, ClinProt magnetic bead enrichment, Nano-LC-ESI-ion trap tandem mass spectrometry immunohistochemical...
ER-Stress and activation of unfolded protein response belong to the major factors involved in chemoresistance cancer cells. In this study we investigated effect shikonin on survival acute myeloid leukemia cells role ER-stress ERP57, a disulfide isomerase, improvement chemotherapy.Using MTT assay studied cytotoxic effects HL-60 The flow cytometry was adopted examine induced mode cell death overall expression alteration resulting from treatment using proteomics methods. Western blotting...
Syndecan-4 (Synd4) is a member of the membrane-spanning, glycocalyx-forming proteoglycan family. It has been suggested that Synd4 participates in renal fibrosis. We compared wild-type and fibrosis-prone endothelial sirtuin 1-deficient (Sirt1endo-/-) mice, latter being model global dysfunction. performed mass spectrometry analysis, which revealed was highly enriched secretome microvascular cells obtained from Sirt1endo-/- mice upon stimulation with transforming growth factor-β1; notably, all...
Yeast cells respond to changes of the environment by complex modifications metabolism. An increase extracellular glucose concentration activates Ras-cAMP pathway. Via a production cAMP this pathway stimulates cAMP-dependent protein kinase (PKA) which is involved in posttranslational regulation key enzymes gluconeogenesis and glycolysis. 6-Phosphofructo-2-kinase (PFK2) catalyzes synthesis fructose 2,6-bisphosphate, most potent activator glycolytic enzyme 6-phosphofructo-1-kinase. We...
The molecular basis of bipolar disorder (BD) is still unknown as the mechanism through which lithium, therapy choice, exerts its effects in treatment BD. So far, no biomarkers exist to facilitate diagnosis BD or evaluation. To investigate whether and with lithium leaves a characteristic signature serum proteome, we used SELDI-TOF MS analyze individual samples from patients treated (BD-plus-Li, n=15) other drugs (BD-minus-Li, n=10) healthy controls (n=15). Interestingly, features 28 kDa (one...
Background. The aim of our study was to investigate the clearance functional proteins by different low-density lipoprotein-apheresis (LDL-A) methods with help proteomic analyses.
Fusarium oxysporum f. sp. Albedinis (Foa) is the causal agent of Bayoud disease, responsible for loss 75% date palm trees in Morocco and posing a threat to its cultivation across North Africa. This study examined ten Foa isolated from various Moroccan locations presence transposable element Fot1 distribution “Secreted Xylem” (SIX) genes. Pathogenicity assays on seedlings revealed varying levels aggressiveness among isolates, with positive correlation between SIX gene count. Highly aggressive...
The whole-cell patch-clamp technique was used to record current responses nucleotides and nucleosides in human embryonic kidney HEK293 cells transfected with the purinergic P2X 3 receptor. When guanosine 5′- O -(3-thiodiphosphate) included into pipette solution, UTP at concentrations that did not alter holding facilitated α,β-methylene ATP (α,β-meATP)-induced current. GTP, but UDP or uridine, had an effect similar of UTP. Compounds known activate protein kinase C (PKC) acted like nucleoside...
Spermatogonial stem cells isolated from the adult mouse testis acquire under certain culture conditions pluripotency and become so-called multipotent germline (maGSCs). They can be differentiated into somatic of three germ layers. We investigated a subset maGSCs ESCs proteomes using cell lines derived two different strains, narrow range immobilized pH gradients to favor detection less abundant proteins, DIGE ensure confident comparison between types. 2-D reference maps in pI ranges 3-6 5-8...